Ohtake Kazumasa 大竹和正

Researcher Number	80593631
Affiliation (Current)	2025: 早稲田大学, 理工学術院, 講師(任期付)
Affiliation (based on the past Project Information) *help	2023: 早稲田大学, 理工学術院, 助教 2018 – 2022: 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 2016 – 2017: 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 研究員 2015: 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 特別研究員 2014: 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, 特別研究員
Review Section/Research Field	Principal Investigator Basic Section 38030:Applied biochemistry-related / Bio-related chemistry / Biomolecular chemistry
Keywords	Principal Investigator 拡張遺伝暗号 / バイオテクノロジー / タンパク質工学 / 非天然型アミノ酸 / 応用生物化学 / 酵素化学 / 人工アミノ酸 / 微生物工学 / 合成生物学 / 微生物による生産 / タンパク質の安定化 / 生体分子の化学修飾

α-ヒドロキシ酸を利用した微生物による有用ペプチド生産系の開発Principal Investigator
- Principal Investigator
  
  大竹和正
- Project Period (FY)
  2019 – 2024
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 38030:Applied biochemistry-related
- Research Institution
  Waseda University
  Institute of Physical and Chemical Research
Development of highly functionalized protein production technology by incorporating multiple non-natural amino acid derivativesPrincipal Investigator
- Principal Investigator
  
  Ohtake Kazumasa
- Project Period (FY)
  2016 – 2018
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Bio-related chemistry
- Research Institution
  Institute of Physical and Chemical Research
Development of novel protein stabilization method with multiple site-selective nonnatural amino acids incorporationPrincipal Investigator
- Principal Investigator
  
  Ohtake Kazumasa
- Project Period (FY)
  2014 – 2015
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Biomolecular chemistry
- Research Institution
  Institute of Physical and Chemical Research

All 2024 2023 2022 2021 2019 2018 2017 2016 2015 2014 Other

All Journal Article Presentation Patent

[Journal Article] Correlation between in vitro and in vivo gene-expression strengths is dependent on bottleneck process2024
- Author(s)
  Toshihiko Enomoto, Kazumasa Ohtake, Naoko Senda, and Daisuke Kiga
- Journal Title
  
  New Generation Computing
  
  Volume: －
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19K05821
[Journal Article] Site‐specific photo‐crosslinking/cleavage for protein?protein interface identification reveals oligomeric assembly of lysosomal‐associated membrane protein type 2A in mammalian cells2023
- Author(s)
  Terasawa Kazue、Seike Tatsuro、Sakamoto Kensaku、Ohtake Kazumasa、Terada Tohru、Iwata Takanori、Watabe Tetsuro、Yokoyama Shigeyuki、Hara‐Yokoyama Miki
- Journal Title
  
  Protein Science
  
  Volume: 32 Issue: 12
- DOI
  10.1002/pro.4823
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K05821, KAKENHI-PROJECT-21K10063
[Journal Article] The two domain architecture of LAMP2A regulates its interaction with Hsc702022
- Author(s)
  Yuta Ikami, Kazue Terasawa, Kensaku Sakamoto, Kazumasa Ohtake, Hiroyuki Harada Tetsuro Watabe, Shigeyuki Yokoyama, Miki Hara-Yokoyam
- Journal Title
  
  Experimental Cell Research
  
  Volume: 411 Issue: 1 Pages: 112986-112986
- DOI
  10.1016/j.yexcr.2021.112986
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18H02416, KAKENHI-PROJECT-19K05821, KAKENHI-PROJECT-21K10063, KAKENHI-PROJECT-22H02591
[Journal Article] Direct homophilic interaction of LAMP2A with the two-domain architecture revealed by site-directed photo-crosslinks and steric hindrances in mammalian cells2021
- Author(s)
  Terasawa Kazue、Kato Yuji、Ikami Yuta、Sakamoto Kensaku、Ohtake Kazumasa、Kusano Seisuke、Tomabechi Yuri、Kukimoto-Niino Mutsuko、Shirouzu Mikako、Guan Jun-Lin、Kobayashi Toshihide、Iwata Takanori、Watabe Tetsuro、Yokoyama Shigeyuki、Hara-Yokoyama Miki
- Journal Title
  
  Autophagy
  
  Volume: 17 Issue: 12 Pages: 1-19
- DOI
  10.1080/15548627.2021.1911017
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-19K05821, KAKENHI-PROJECT-17K12005, KAKENHI-PROJECT-18H02416
[Journal Article] An expanded genetic code facilitates antibody chemical conjugation involving the lambda light chain2021
- Author(s)
  Kato Akifumi、Ohtake Kazumasa、Tanaka Yoshitaka、Yokoyama Shigeyuki、Sakamoto Kensaku、Shiraishi Yasuhisa
- Journal Title
  
  Biochemical and Biophysical Research Communications
  
  Volume: 546 Pages: 35-39
- DOI
  10.1016/j.bbrc.2021.02.005
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K05821, KAKENHI-PROJECT-18H02416
[Journal Article] Variants of the industrially relevant protease KP-43 with suppressed activity under alkaline conditions developed using expanded genetic codes2019
- Author(s)
  Osamura Tatsuya、Okuda Mitsuyoshi、Yamaguchi Atsushi、Ohtake Kazumasa、Sakamoto Kensaku、Takimura Yasushi
- Journal Title
  
  Biochemistry and Biophysics Reports
  
  Volume: 17 Pages: 93-96
- DOI
  10.1016/j.bbrep.2018.12.001
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K05821
[Journal Article] Pyrrolysyl-tRNA synthetase with a unique architecture enhances the availability of lysine derivatives in synthetic genetic codes2018
- Author(s)
  A. Yamaguchi, F. Iraha, K. Ohtake, K. Sakamoto
- Journal Title
  
  Molcules
  
  Volume: 23 Issue: 10 Pages: 2460-2460
- DOI
  10.3390/molecules23102460
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18H02416, KAKENHI-PROJECT-16K21631
[Journal Article] Engineering an Automaturing Transglutaminase with Enhanced Thermostability by Genetic Code Expansion with Two Codon Reassignments2018
- Author(s)
  Ohtake Kazumasa、Mukai Takahito、Iraha Fumie、Takahashi Mihoko、Haruna Ken-ichi、Date Masayo、Yokoyama Keiichi、Sakamoto Kensaku
- Journal Title
  
  ACS Synthetic Biology
  
  Volume: 7 Issue: 9 Pages: 2170-2176
- DOI
  10.1021/acssynbio.8b00157
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16K21631
[Journal Article] Extensive Survey of Antibody Invariant Positions for Efficient Chemical Conjugation Using Expanded Genetic Codes2017
- Author(s)
  Kato Akifumi、Kuratani Mitsuo、Yanagisawa Tatsuo、Ohtake Kazumasa、Hayashi Akiko、Amano Yoshimi、Kimura Kaname、Yokoyama Shigeyuki、Sakamoto Kensaku、Shiraishi Yasuhisa
- Journal Title
  
  Bioconjugate Chemistry
  
  Volume: 28 Issue: 8 Pages: 2099-2108
- DOI
  10.1021/acs.bioconjchem.7b00265
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16K21631, KAKENHI-PROJECT-16K05859
[Journal Article] 新規アミノ酸の導入によるタンパク質の安定化技術2016
- Author(s)
  大竹和正、坂本健作
- Journal Title
  
  バイオサイエンスとインダストリー
  
  Volume: 74(1) Pages: 34-36
- Data Source
  KAKENHI-PROJECT-26870853
[Journal Article] Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates2016
- Author(s)
  Atsushi Yamaguchi, Takayoshi Matsuda, Kazumasa Ohtake, Tatsuo Yanagisawa, Shigeyuki Yokoyama, Yoshihisa Fujiwara, Takayoshi Watanabe, Takahiro Hohsaka, Kensaku Sakamoto
- Journal Title
  
  Bioconjugate Chemistry
  
  Volume: 27 Issue: 1 Pages: 198-206
- DOI
  10.1021/acs.bioconjchem.5b00602
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-15K16561, KAKENHI-ORGANIZER-25102001, KAKENHI-PLANNED-25102006, KAKENHI-PROJECT-26870853, KAKENHI-PROJECT-26291035
[Journal Article] Protein stabilization utilizing a redefined codon2015
- Author(s)
  Ohtake, K., Yamaguchi, A., Mukai, T., Kashimura, H., Hirano, N., Haruki, M., Kohashi, S., Yamagishi, K., Murayama. K., Tomabechi, Y., Itagaki, T., Akasaka, R., Kawazoe, M., Takemoto, C., Shirouzu, M., Yokoyama, S. & Sakamoto, K.
- Journal Title
  
  Scientific Reports
  
  Volume: 5 Issue: 1 Pages: 9762-9762
- DOI
  10.1038/srep09762
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-26870853, KAKENHI-PROJECT-26291035
[Journal Article] Ubiquitin acetylation inhibits polyubiquitin chain elongation.2015
- Author(s)
  Ohtake F, Saeki Y, Sakamoto K, Ohtake K, Nishikawa H, Tsuchiya H, Ohta T, Tanaka K, Kanno J.
- Journal Title
  
  EMBO Rep.
  
  Volume: 16 Issue: 2 Pages: 192-201
- DOI
  10.15252/embr.201439152
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PLANNED-24112005, KAKENHI-PLANNED-24112008, KAKENHI-PROJECT-26290042, KAKENHI-PROJECT-26291035, KAKENHI-PROJECT-26670730, KAKENHI-PROJECT-26870853, KAKENHI-PLANNED-24112004, KAKENHI-PROJECT-26000014
[Journal Article] Highly reproductive Escherichia coli cells with a blank assignment for the UAG codon2015
- Author(s)
  Mukai, T., Hoshi, H.; Ohtake, K., Takahashi, M., Yamaguchi, A., Hayashi, A., Yokoyama, S. & Sakamoto, K.
- Journal Title
  
  Scientific Reports
  
  Volume: 5 Issue: 1 Pages: 9699-9699
- DOI
  10.1038/srep09699
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-26870853, KAKENHI-PROJECT-26291035
[Journal Article] Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli2015
- Author(s)
  Mukai, T., Yamaguchi, A., Ohtake, K., Takahashi, M., Hayashi, A., Iraha, F., Kira, S., Yanagisawa, T., Yokoyama, S., Hoshi, H., Kobayashi, T., Sakamoto, K.
- Journal Title
  
  Nucleic Acids Research
  
  Volume: 43(16) Issue: 16 Pages: 8111-8122
- DOI
  10.1093/nar/gkv787
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-26870853, KAKENHI-PROJECT-26291035
[Patent] タンパク質における別のタンパク質と相互作用する部分の特定方法2023
- Inventor(s)
  横山三紀、寺澤和恵、横山茂之、坂本健作、大竹和正
- Industrial Property Rights Holder
  横山三紀、寺澤和恵、横山茂之、坂本健作、大竹和正
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2023-098438
- Filing Date
  2023
- Data Source
  KAKENHI-PROJECT-19K05821
[Patent] 変異ＶＨＨ抗体及びその製造方法2018
- Inventor(s)
  坂本健作、大竹和正他
- Industrial Property Rights Holder
  坂本健作、大竹和正他
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2017-124289
- Filing Date
  2018
- Data Source
  KAKENHI-PROJECT-16K21631
[Patent] 非天然アミノ酸導入抗体2016
- Inventor(s)
  横山茂之坂本健作柳沢達男大竹和正 (他計10名)
- Industrial Property Rights Holder
  横山茂之坂本健作柳沢達男大竹和正 (他計10名)
- Industrial Property Rights Type
  特許
- Filing Date
  2016-08-18
- Overseas
- Data Source
  KAKENHI-PROJECT-16K21631
[Presentation] 非アミノ酸をコード化した遺伝暗号の下での大腸菌進化2023
- Author(s)
  坂本健作、大竹和正、芝井厚、古澤力
- Organizer
  第45 回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-19K05821
[Presentation] 新型コロナウイルス変異株に対応する次世代型ACE2-Fc 製剤の開発2023
- Author(s)
  高田理彩、尾上遥南、鈴木喬介、石本憲司、大竹和正、坂本健作、吉岡靖雄、中川晋作、樋野展正
- Organizer
  第45 回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-19K05821
[Presentation] 非アミノ酸を含む遺伝暗号の開発と大腸菌進化への影響2022
- Author(s)
  大竹和正
- Organizer
  第16回無細胞生命科学研究会
- Data Source
  KAKENHI-PROJECT-19K05821
[Presentation] 二重コドン再定義を用いたトランスグルタミナーゼの高機能化2018
- Author(s)
  大竹和正、高橋美穂子、春名健一、坂本健作
- Organizer
  「細胞を創る」研究会 11.0(仙台市)
- Data Source
  KAKENHI-PROJECT-16K21631
[Presentation] 二種非天然型アミノ酸同時導入による高機能化タンパク質生産2018
- Author(s)
  大竹和正、春名健一、坂本健作
- Organizer
  日本農芸化学会2017年度大会(名古屋市)
- Data Source
  KAKENHI-PROJECT-16K21631
[Presentation] 大腸菌コドン再定義株を用いた二種非天然型アミノ酸導入によるタンパク質工学2017
- Author(s)
  大竹和正、春名健一、坂本健作
- Organizer
  第17回蛋白質科学会年会(仙台市)
- Data Source
  KAKENHI-PROJECT-16K21631
[Presentation] 二種非天然型アミノ酸同時導入による非酵素的活性化可能な安定化トランスグルタミナーゼの生産2017
- Author(s)
  大竹和正, 春名健一, 坂本健作
- Organizer
  日本農芸化学会2017年度大会
- Place of Presentation
  京都女子大学(京都府京都市)
- Year and Date
  2017-03-19
- Data Source
  KAKENHI-PROJECT-16K21631
[Presentation] コドン再定義株を用いた二種非天然型アミノ酸同時導入2017
- Author(s)
  大竹和正、春名健一、坂本健作
- Organizer
  第12回無細胞生命科学研究会(柏市)
- Data Source
  KAKENHI-PROJECT-16K21631
[Presentation] 大腸菌を用いた非天然型アミノ酸導入～コドン再定義が拓く新たな可能性2017
- Author(s)
  大竹和正
- Organizer
  2017年度生物工学若手研究者の集い（若手会）夏のセミナー(福山市)
- Invited
- Data Source
  KAKENHI-PROJECT-16K21631
[Presentation] 大腸菌コドン再定義株を用いた二種非天然型アミノ酸同時導入法の開発2016
- Author(s)
  大竹和正
- Organizer
  日本農芸化学会関東支部2016年度第2回支部例会
- Place of Presentation
  日本獣医生命科学大学(東京都武蔵野市)
- Year and Date
  2016-09-10
- Invited
- Data Source
  KAKENHI-PROJECT-16K21631
[Presentation] 大腸菌コドン再定義株を用いた二種非天然型アミノ酸同時導入法の開発2016
- Author(s)
  大竹和正、春名健一、坂本健作
- Organizer
  日本農芸化学会2016年度大会
- Place of Presentation
  札幌コンベンションセンター・札幌市・北海道
- Year and Date
  2016-03-30
- Data Source
  KAKENHI-PROJECT-26870853
[Presentation] In vivo engineering of the genetic code with synthetic amino acids2015
- Author(s)
  坂本健作、大竹和正、山口純、向井崇人
- Organizer
  BMB2015(第38回日本分子生物学会年会第88回日本生化学会大会合同大会)
- Place of Presentation
  神戸ポートアイランド・神戸市・兵庫県
- Year and Date
  2015-12-02
- Data Source
  KAKENHI-PROJECT-26870853
[Presentation] 大腸菌コドン再定義株がもたらす新たなタンパク質工学の展開2015
- Author(s)
  大竹和正、山口純、春名健一、坂本健作
- Organizer
  BMB2015(第38回日本分子生物学会年会第88回日本生化学会大会合同大会)
- Place of Presentation
  神戸ポートアイランド・神戸市・兵庫県
- Year and Date
  2015-12-02
- Data Source
  KAKENHI-PROJECT-26870853
[Presentation] DNA 情報をタンパク質に変換するルールの人為的な改変と蛋白工学への応用2015
- Author(s)
  坂本健作、大竹和正
- Organizer
  第67回日本生物工学会大会
- Place of Presentation
  城山観光ホテル・鹿児島市・鹿児島県
- Year and Date
  2015-10-28
- Data Source
  KAKENHI-PROJECT-26870853
[Presentation] 部位特異的多箇所へのハロゲン化チロシン導入による新規タンパク質安定化法2015
- Author(s)
  大竹和正, 山口純, 春木満, 坂本健作
- Organizer
  日本農芸化学会 2015年度大会
- Place of Presentation
  岡山大学津島キャンパス
- Year and Date
  2015-03-27
- Data Source
  KAKENHI-PROJECT-26870853
[Presentation] ハロゲン化チロシンの多箇所への部位特異的導入によるタンパク質の安定化2014
- Author(s)
  大竹和正, 山口純, 春木満, 山岸賢司, 村山和隆, 白水美香子, 横山茂之, 坂本健作
- Organizer
  第52回日本生物物理学会年会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2014-09-25
- Data Source
  KAKENHI-PROJECT-26870853
[Presentation] 大腸菌コドン再定義が拓くタンパク質工学の新たな可能性
- Author(s)
  大竹和正, 山口純, 坂本健作
- Organizer
  「細胞を創る」研究会 7.0
- Place of Presentation
  東京大学弥生キャンパス
- Year and Date
  2014-11-13 – 2014-11-14
- Data Source
  KAKENHI-PROJECT-26870853

1. 太田智彦

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
2. 坂本健作

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
3. 渡邉貴嘉

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Ohtake Kazumasa 大竹 和正

Research Projects

Research Products

Co-Researchers

α-ヒドロキシ酸を利用した微生物による有用ペプチド生産系の開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of highly functionalized protein production technology by incorporating multiple non-natural amino acid derivativesPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of novel protein stabilization method with multiple site-selective nonnatural amino acids incorporationPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] Correlation between in vitro and in vivo gene-expression strengths is dependent on bottleneck process2024

Author(s)

Journal Title

Data Source

[Journal Article] Site‐specific photo‐crosslinking/cleavage for protein?protein interface identification reveals oligomeric assembly of lysosomal‐associated membrane protein type 2A in mammalian cells2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] The two domain architecture of LAMP2A regulates its interaction with Hsc702022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Direct homophilic interaction of LAMP2A with the two-domain architecture revealed by site-directed photo-crosslinks and steric hindrances in mammalian cells2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] An expanded genetic code facilitates antibody chemical conjugation involving the lambda light chain2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Variants of the industrially relevant protease KP-43 with suppressed activity under alkaline conditions developed using expanded genetic codes2019

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Pyrrolysyl-tRNA synthetase with a unique architecture enhances the availability of lysine derivatives in synthetic genetic codes2018

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Engineering an Automaturing Transglutaminase with Enhanced Thermostability by Genetic Code Expansion with Two Codon Reassignments2018

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Extensive Survey of Antibody Invariant Positions for Efficient Chemical Conjugation Using Expanded Genetic Codes2017

Author(s)

Journal Title

DOI

Data Source

[Journal Article] 新規アミノ酸の導入によるタンパク質の安定化技術2016

Author(s)

Journal Title

Data Source

[Journal Article] Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates2016

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Protein stabilization utilizing a redefined codon2015

Author(s)

Journal Title

DOI

Data Source

Ohtake Kazumasa 大竹和正