Okubo Yusuke 大久保佑亮

Researcher Number	80596247
Other IDs
Affiliation (Current)	2025: 国立医薬品食品衛生研究所, 毒性部, 室長
Affiliation (based on the past Project Information) *help	2024 – 2025: 国立医薬品食品衛生研究所, 毒性部, 室長 2018 – 2023: 国立医薬品食品衛生研究所, 毒性部, 主任研究官 2017: 国立医薬品食品衛生研究所, 毒性部, 研究員 2014 – 2015: 国立医薬品食品衛生研究所, 毒性部, 主任研究官 2013: 国立医薬品食品衛生研究所, 毒性部, 研究員
Review Section/Research Field	Principal Investigator Basic Section 63040:Environmental impact assessment-related / Medium-sized Section 64:Environmental conservation measure and related fields / Basic Section 64010:Environmental load and risk assessment-related / Medium-sized Section 90:Biomedical engineering and related fields / 0704:Neuroscience, brain sciences, and related fields / Developmental biology Except Principal Investigator Medium-sized Section 20:Mechanical dynamics, robotics, and related fields / Basic Section 27040:Biofunction and bioprocess engineering-related / Transformative Research Areas, Section (II) / Risk sciences of radiation and chemicals
Keywords	Principal Investigator Deltaシグナル / Notchシグナル / 自己複製 / 神経新生 / Multivalent ligands / Notch-Deltaシグナル / 毒性評価技術 / マルチモーダルバイタルサイン / ウェアラブルデバイス / 毒性試験 … More / 動物実験代替法 / リアルタイムルシフェレースアッセイ / in vitro試験法 / 多色リアルタイム発光測定 / シグナルかく乱 / 化学物質 / 催奇形性 / シグナルの賦活化 / AAV / Delta-Notchシグナル / 神経幹細胞 / ドーパミン作動性神経細胞 / iPS細胞 / ドーパミン神経細胞 / クラスター化タンパク質 / Notch－Deltaシグナル / 神経前駆細胞 / 体節形成 / 神経発生 / 発生 / 後根神経節 / 後根神経節発生 … More Except Principal Investigator 組織培養 / ティッシュエンジニアリング / 増殖培養 / 三次元培養 / マイクロデバイス / スマートデバイス / 電子システム / ハイドロゲル / 液体金属 / 原基 / 脱毛症 / 毛包 / 生物化学工学 / 電気化学 / 毛包幹細胞 / バイオプリンタ / 毛包原基 / 毛髪再生医療 / バイオプリンター / 毛乳頭細胞 / 毛包上皮幹細胞 / 毛髪の再生医療 / チップデバイス / 成熟化 / 分化誘導 / ヒトiPS細胞 / 細胞組織工学 / 小腸 / 肝臓 / 毛包オルガノイド / オルガノイド培養 / 微細加工 / Organs on a chip / Body on a chip / オルガノイド / レポーターマウス / エピジェネティック毒性 / トキシコロジー / 有害化学物質 / レポーター / 毒性試験 / エピジェネティクス / ゲノム Less

リアルタイム生細胞解析による催奇形性誘発機序の解明Principal Investigator
- Principal Investigator
  
  大久保佑亮
- Project Period (FY)
  2025 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 63040:Environmental impact assessment-related
- Research Institution
  National Institute of Health Sciences
DXによるin vivo毒性評価技術の発展的継承と一般化Principal Investigator
- Principal Investigator
  
  大久保佑亮
- Project Period (FY)
  2023 – 2026
- Research Category
  
  Grant-in-Aid for Challenging Research (Pioneering)
- Review Section
  
  Medium-sized Section 64:Environmental conservation measure and related fields
- Research Institution
  National Institute of Health Sciences
ハイドロゲルと液体金属から構成される超ソフト電子機械システムに関する研究
- Principal Investigator
  
  太田裕貴
- Project Period (FY)
  2022 – 2025
- Research Category
  
  Grant-in-Aid for Challenging Research (Pioneering)
- Review Section
  
  Medium-sized Section 20:Mechanical dynamics, robotics, and related fields
- Research Institution
  Yokohama National University
化学物質のシグナルかく乱作用を基にした新規in vitro催奇形性評価原理の解明Principal Investigator
- Principal Investigator
  
  大久保佑亮
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 64010:Environmental load and risk assessment-related
- Research Institution
  National Institute of Health Sciences
Controlling bi-directional Notch-Delta signalling in hippocampal neural stem cells to enhance the self-renewal and the neurogenesisPrincipal Investigator
- Principal Investigator
  
  OKUBO YUSUKE
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 90:Biomedical engineering and related fields
- Research Institution
  National Institute of Health Sciences
Engineering organoids on a chip based on tissue engineering approaches
- Principal Investigator
  
  Fukuda Junji
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Transformative Research Areas (B)
- Review Section
  
  Transformative Research Areas, Section (II)
- Research Institution
  Yokohama National University
Three-dimensional cutlure for hari regenerative medicine
- Principal Investigator
  
  Fukuda Junji
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 27040:Biofunction and bioprocess engineering-related
- Research Institution
  Yokohama National University
Controlling bi-directional Notch-Delta signaling in human iPSC derived dopaminergic neuron using multivalent protein.Principal Investigator
- Principal Investigator
  
  Okubo Yusuke
- Project Period (FY)
  2018 – 2019
- Research Category
  
  Grant-in-Aid for Research Activity Start-up
- Review Section
  
  0704:Neuroscience, brain sciences, and related fields
- Research Institution
  National Institute of Health Sciences
Development of reporter mice enabling detection of epigenetic toxicity in the conventional toxicity tests
- Principal Investigator
  
  Igarashi Katsuhide
- Project Period (FY)
  2013 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Risk sciences of radiation and chemicals
- Research Institution
  Hoshi University
  National Institute of Health Sciences
Analysis of tissue developments regulated by bi-directional Notch-Delta signalPrincipal Investigator
- Principal Investigator
  
  Okubo Yusuke
- Project Period (FY)
  2013 – 2015
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Developmental biology
- Research Institution
  National Institute of Health Sciences

All 2024 2023 2022 2021 2019 2015 2014 Other

All Journal Article Presentation

[Journal Article] 生細胞ルシフェラーゼアッセイを用いたFGFシグナルかく乱作用解析による発生毒性評価2023
- Author(s)
  大久保　佑亮、福田　淳二
- Journal Title
  
  生化学みにれびゅう
  
  Volume: 95-2 Pages: 1-6
- Data Source
  KAKENHI-PROJECT-23K25021
[Journal Article] Integrated fibroblast growth factor signal disruptions in human iPS cells for prediction of teratogenic toxicity of chemicals2022
- Author(s)
  Kanno Seiya、Okubo Yusuke、Kageyama Tatsuto、Yan Lei、Fukuda Junji
- Journal Title
  
  Journal of Bioscience and Bioengineering
  
  Volume: 133 Issue: 3 Pages: 291-299
- DOI
  10.1016/j.jbiosc.2021.12.006
- NAID
  210000159560
- Peer Reviewed
- Data Source
  KAKENHI-PLANNED-20H05744
[Journal Article] Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruption2022
- Author(s)
  Kanno Seiya、Okubo Yusuke、Kageyama Tatsuto、Yan Lei、Kitajima Satoshi、Fukuda Junji
- Journal Title
  
  iScience
  
  Volume: 25 Issue: 2 Pages: 103770-103770
- DOI
  10.1016/j.isci.2022.103770
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PLANNED-20H05744, KAKENHI-PROJECT-20J15648
[Journal Article] Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs2022
- Author(s)
  Kanno Seiya、Mizota Kashu、Okubo Yusuke、Kageyama Tatsuto、Yan Lei、Fukuda Junji
- Journal Title
  
  STAR Protocols
  
  Volume: 3 Issue: 2 Pages: 101439-101439
- DOI
  10.1016/j.xpro.2022.101439
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PLANNED-20H05744, KAKENHI-PROJECT-23K25021
[Journal Article] Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction2022
- Author(s)
  Kageyama Tatsuto、Shimizu Akihiro、Anakama Riki、Nakajima Rikuma、Suzuki Kohei、Okubo Yusuke、Fukuda Junji
- Journal Title
  
  Science Advances
  
  Volume: 8 Issue: 42
- DOI
  10.1126/sciadv.add4603
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PLANNED-20H05744, KAKENHI-PROJECT-20H02535
[Journal Article] Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways2021
- Author(s)
  Okubo Yusuke、Ohtake Fumiaki、Igarashi Katsuhide、Yasuhiko Yukuto、Hirabayashi Yoko、Saga Yumiko、Kanno Jun
- Journal Title
  
  Development
  
  Volume: 148 Issue: 19 Pages: 193664-193664
- DOI
  10.1242/dev.193664
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PLANNED-20H05744, KAKENHI-PROJECT-21H03604, KAKENHI-PROJECT-20K21913
[Journal Article] タンパク質のクラスター化によるシグナル増強！？2019
- Author(s)
  大久保　佑亮
- Journal Title
  
  生物工学会誌、バイオミディア
  
  Volume: 97 Pages: 90-90
- Data Source
  KAKENHI-PROJECT-19K21216
[Journal Article] タンパク質のクラスター化によるシグナル増強！？2019
- Author(s)
  大久保　佑亮
- Journal Title
  
  生物工学会誌
  
  Volume: 97 Pages: 90-90
- Data Source
  KAKENHI-PROJECT-19K21216
[Presentation] シグナルかく乱作用のダイナミクスを基にした in vitro 発生毒性試験法2024
- Author(s)
  大久保　佑亮
- Organizer
  「生殖発生毒性試験代替法の現状」シンポジウム．
- Invited
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] Development of a wearable device used to monitor multimodal vital signs in conscious, unrestrained rats2024
- Author(s)
  Okubo Y., Kojima S., Ohara I., Min M., Taquahashi Y., Tochinai R. Ota H.
- Organizer
  Society of Toxicology
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K17469
[Presentation] 生細胞リアルタイム発光システムを用いた高精度・ハイスループットな新規発生毒性試験法2023
- Author(s)
  大久保　佑亮
- Organizer
  AMEDキャタリストユニット　第11回Top Runners in TRS．
- Invited
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] シグナルかく乱のリアルタイム計測を基にした in vitro 発生毒性試験2023
- Author(s)
  大久保　佑亮
- Organizer
  第9 回日本医療研究開発機構レギュラトリーサイエンス公開シンポジウムレギュラトリーサイエンスにおける動物試験代替法の発展～細胞培養技術の進化と展望～
- Invited
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] シグナル伝達のかく乱計測による発生毒性予測法の開発2023
- Author(s)
  大久保佑亮
- Organizer
  第50回日本毒性学会
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] 覚醒化非拘束ラット用マルチバイタル・活動量測定ウェアブルデバイスの開発2023
- Author(s)
  小島　竣平、小原　息吹、高野　珠実、磯田　豊，馬　敏、栃内　亮太、大久保　佑亮、高橋　祐次、太田　裕貴
- Organizer
  第50回日本毒性学会学術年会
- Data Source
  KAKENHI-PROJECT-23K17469
[Presentation] Developmental toxicity test using human iPS cells based on chemical induced signal disruption dynamics.2023
- Author(s)
  Okubo Y., Mizota K., Shibata1 M., Hirabayashi Y., Nakajima Y., Fukuda J.
- Organizer
  The 56th Annual Meeting of the Japanese Society of Developmental Biologists
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] ヒトiPS細胞を用いたシグナルかく乱作用のダイナミクスに基づく発生毒性試験法の開発2023
- Author(s)
  大久保　佑亮
- Organizer
  幹細胞を用いた化学物質リスク情報共有化コンソーシアム2023年度年会
- Invited
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] Developmental toxicity detection via dynamics of FGF-SRF signal disruption in human iPSC-based assay2023
- Author(s)
  Yusuke Okubo
- Organizer
  13TH Global Summit on Regulatory Science (GSRS23) In-Person Annual conference
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] シグナルかく乱作用のダイナミクスに基づくヒト発生毒性の評価法とその発達神経毒性への応用2023
- Author(s)
  大久保　佑亮
- Organizer
  第63回日本先天異常学会学術集会
- Invited
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] DEVELOPMENTAL TOXICITY TEST USING HUMAN IPS CELLS BASED ON SIGNAL DISRUPTIONS INDUCED BY CHEMICAL SUBSTANCES2023
- Author(s)
  Okubo Y., Mizota K., Shibata1 M., Ohara R., Kitajima S., Hirabayashi Y., Nakajima Y., Fukuda J.
- Organizer
  12th World Congress on Alternatives and Animal Use in the Life Sciences (WC12)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] ヒトiPS細胞を用いたシグナル伝達かく乱作用のダイナミクスに基づく高精度かつ網羅的ヒト発生毒性試験法の開発．2022
- Author(s)
  大久保佑亮、菅野聖世、北嶋聡、平林容子、福田淳二
- Organizer
  第49回日本毒性学会
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] Establishment of developmental toxicity test based on the integration of FGF signal disruption effects for safety evaluation of drugs and chemicals using human iPS cells．2022
- Author(s)
  Okubo Y., Kanno S. Kitajima S., Hirabayashi Y., Fukuda J.
- Organizer
  The 55th Annual Meeting of the Japanese Society of Developmental Biologist.
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] ヒトiPS細胞を用いたシグナルかく乱作用のダイナミクスに基づく高精度・網羅的な発生毒性試験法の開発2022
- Author(s)
  大久保佑亮、菅野聖世、北嶋聡、平林容子、福田淳二
- Organizer
  日本動物実験代替法学会
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] ヒトiPS細胞を用いたシグナルかく乱作用のダイナミクスに基づく高精度・網羅的な発生毒性試験法の開発2022
- Author(s)
  大久保佑亮
- Organizer
  日本先天異常学会
- Data Source
  KAKENHI-PLANNED-20H05744
[Presentation] ヒトiPS細胞を用いたシグナルかく乱作用のダイナミクスに基づく高精度・網羅的な発生毒性試験法の開発．2022
- Author(s)
  大久保佑亮、菅野聖世、北嶋聡、平林容子、福田淳二
- Organizer
  第62回日本先天異常学会学術集会
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] リアルタイム発光測定法を用いたシグナルかく乱作用のダイナミクス測定による医薬品の発生毒性の評価2022
- Author(s)
  大久保佑亮、溝田華柊、平林容子、中島芳浩、福田淳二
- Organizer
  第45回日本分子生物学会年会発生生物学会
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] Establishment of developmental toxicity test based on the integration of FGF signal disruption effects for safety evaluation of drugs and chemicals using human iPS cells.2022
- Author(s)
  Okubo Y., Kanno S. Kitajima S., Hirabayashi Y., Fukuda J.
- Organizer
  IUTOX XVIth International Congress of Toxicology, Maastricht, Netherlands.
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] Establishment of developmental toxicity test based on the integration of FGF signal disruption effects for safety evaluation of drugs and chemicals using human iPS cells.2022
- Author(s)
  Okubo Y., Kanno S., Kitajima S., Hirabayashi Y., Fukuda J
- Organizer
  ICCA-LRI & NITE Workshop.
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K25021
[Presentation] ヒトiPS細胞を用いたシグナルかく乱を指標とする催奇形性物質のスクリーニング法2021
- Author(s)
  菅野聖世、大久保佑亮、北嶋聡、福田淳二
- Organizer
  日本動物実験代替法学会第34回
- Data Source
  KAKENHI-PLANNED-20H05744
[Presentation] Cleaved Dll1 intracellular domain fine-tunes DRG development as the reverse signal of Notch2015
- Author(s)
  Okubo Y., Ohtake F., Igarashi K., Yasuhiko Y., Saga Y., Kanno J.
- Organizer
  BMB2015
- Place of Presentation
  神戸ポートアイランド
- Year and Date
  2015-12-02
- Invited
- Data Source
  KAKENHI-PROJECT-25840096
[Presentation] 後根神経節の神経形成における双方向性のNotch-Deltaシグナルの機能解析2014
- Author(s)
  大久保佑亮
- Organizer
  日本発生生物学会　秋季シンポジウム　2013
- Place of Presentation
  兵庫県神戸市
- Data Source
  KAKENHI-PROJECT-25840096
[Presentation] Analysis of the Delta signaling as the reverse signaling of Notch in mouse development
- Author(s)
  Yusuke Okubo, Katsuhide Igarashi, Yumiko Saga, Jun Kanno
- Organizer
  47th Annual Meeting of the Japanese Society of Developmental Biologist
- Place of Presentation
  愛知県名古屋市
- Year and Date
  2014-05-27 – 2014-05-30
- Data Source
  KAKENHI-PROJECT-25840096
[Presentation] Analysis of the Delta signaling as the reverse signaling of Notch in mouse development
- Author(s)
  Yusuke Okubo, Katsuhide Igarashi, Yumiko Saga, Jun Kanno
- Organizer
  Gordon Research Conference. Notch signaling in Development, Regeneration & Disease
- Place of Presentation
  米国メイン州ルインストン市
- Year and Date
  2014-07-19 – 2014-07-25
- Data Source
  KAKENHI-PROJECT-25840096

1. Fukuda Junji (80431675)

# of Collaborated Projects: 3 results

# of Collaborated Products: 15 results
2. 太田裕貴 (30528435)

# of Collaborated Projects: 2 results

# of Collaborated Products: 2 results
3. 栃内亮太 (90833997)

# of Collaborated Projects: 2 results

# of Collaborated Products: 2 results
4. Igarashi Katsuhide (30342885)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. 大塚まき (40734372)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
6. 山本直樹 (50757432)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. 西川昌輝 (40843149)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. 遠山周吾 (90528192)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. 景山達斗 (40822177)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. 竹村泰司 (30251763)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
11. 中島芳浩 (10291080)

# of Collaborated Projects: 1 results

# of Collaborated Products: 3 results
12. 平林容子 (30291115)

# of Collaborated Projects: 1 results

# of Collaborated Products: 9 results

Okubo Yusuke 大久保 佑亮

Research Projects

Research Products

Co-Researchers

リアルタイム生細胞解析による催奇形性誘発機序の解明Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

DXによるin vivo毒性評価技術の発展的継承と一般化Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

ハイドロゲルと液体金属から構成される超ソフト電子機械システムに関する研究

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

化学物質のシグナルかく乱作用を基にした新規in vitro催奇形性評価原理の解明Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Controlling bi-directional Notch-Delta signalling in hippocampal neural stem cells to enhance the self-renewal and the neurogenesisPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Engineering organoids on a chip based on tissue engineering approaches

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Three-dimensional cutlure for hari regenerative medicine

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Controlling bi-directional Notch-Delta signaling in human iPSC derived dopaminergic neuron using multivalent protein.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of reporter mice enabling detection of epigenetic toxicity in the conventional toxicity tests

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Analysis of tissue developments regulated by bi-directional Notch-Delta signalPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] 生細胞ルシフェラーゼアッセイを用いたFGFシグナルかく乱作用解析による発生毒性評価2023

Author(s)

Journal Title

Data Source

[Journal Article] Integrated fibroblast growth factor signal disruptions in human iPS cells for prediction of teratogenic toxicity of chemicals2022

Author(s)

Journal Title

DOI

NAID

Data Source

[Journal Article] Establishment of a developmental toxicity assay based on human iPSC reporter to detect FGF signal disruption2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs2022

Okubo Yusuke 大久保佑亮