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KOIKE Tohru  小池 透

ORCIDConnect your ORCID iD *help
Researcher Number 90186586
Other IDs
External Links
Affiliation (based on the past Project Information) *help 2019 – 2022: 広島大学, 医系科学研究科(薬), 教授
2017 – 2018: 広島大学, 医歯薬保健学研究科(薬), 教授
2014 – 2016: 広島大学, 医歯薬保健学研究院(薬), 教授
2012 – 2013: 広島大学, 医歯薬保健学研究院, 教授
2011: 広島大学, 大学院・医歯薬学総合研究科, 教授 … More
2010: 広島大学, 大学院・医歯薬総合研究科, 教授
2002 – 2009: Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授
2006: Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院医歯薬学総合研究科, 教授
1998 – 2001: 広島大学, 医学部, 教授
1991 – 1997: 広島大学, 医学部, 助教授
1988 – 1990: 広島大学, 医学部, 講師
1989: Hiroshima University School of Medicine Associate Professor, 医学部, 平成1. 縲怩W月講師,
1989: 広島大学, 医学部, 講師・助教授 Less
Review Section/Research Field
Principal Investigator
医薬分子機能学 / Physical pharmacy / Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related / 広領域 / Drug development chemistry / Science and Engineering
Except Principal Investigator
Bioorganic chemistry / Chemical pharmacy / 分析・地球化学 / 分離・精製・検出法
Keywords
Principal Investigator
リン酸化タンパク質 / 亜鉛酵素 / 人工酵素 / プロテオミクス / 大環状ポリアミン / 蛍光分析法 / フォスファターゼ / キナーゼ / Phos-tag / 蛍光分析 … More / リン酸化プロテオミクス / ケミカルバイオロジー / 生体機能制御分子 / 亜鉛錯体 / フォスタグ技術 / Macrocyclic Polyamine / 環状ポリアミン / Artificila Enzyme / Protease / Intelligent molecule / プロテアーゼ / 亜鉛化合物 / フォスタグ / リン酸化分子 / リン酸化反応 / シグナル伝達 / キナーゼプロファイリング / インテリジェント分子 / Thio-tag / タンパク質の翻訳語修飾 / リン酸化生体ぶんし / チオタグ / タンパク質の翻訳後修飾 / リン酸化生体分子 / マイクロビーズ法 / 翻訳後修飾 / チオタグ技術 / リン酸化シグナル分子 / 二成分伝達系 / Phos-tag技術 / タンパク質のリン酸化 / Kinase Profiling / Affinity Electrophoresis / Western Analysis / Metal Complex / Postgenome Research / Phosphoproteomics / SPR分析 / アフィニティークロマトグラフィー / ゲル蛍光染色 / アフィニティー電気泳動 / ウエスタン解析 / 金属錯体 / ポストゲノム解析 / Chelate / Fluorometry / Zinc(II) Ion / Zinc Fuorophore / キレート試薬 / 生体内亜鉛イオン / 亜鉛蛍光プローブ / Phosphatase / フォスフォプロテオーム / Environment-friendly Catalyst / Zinc Enzyme / 環境触媒 / Artificila Phosphatase / Intelligent Molecule / Phosphoryl Protein / 人工フォスファターゼ / リン酸化 / 消光性分子 / タンパク質 / タンパク質リン酸化 / キナーゼ反応 / 磁気ビーズ / FRET / ナノバイオ / リン酸化蛋白質 / フォスファターゼ阻害剤 / キナーゼ阻害剤 / 電気泳動法 / リン酸化調節因子 / 不溶性タンパク質 / プオテオーム / 人工シャペロン / プロテオーム / 分子認識 / 多核錯体 / 超分子 / 自己集積 / β-ラクタマーゼ / リボヌクレアーゼ … More
Except Principal Investigator
大環状ポリアミン / microphyllone / カルシウムチャネル / 坑塩基球 / マスト細胞 / RBL-2H3細胞 / 坑アレルギー薬 / Macrocyclic Polyamine / 電位応答 / 金メッキ剤 / 亜鉛酵素 / Cyclic Voltammetry / Permeability Change / Langmuir-Blodgett (LB) Membrane Sensor / Potentiometry / Dicarboxylic Acid / Adenosine Nucleotide / Anion-selective Electrode / 中性分子センサー / ジカルボン酸陰イオンセンサー / ATP^<4->センサー / 単分子累積膜 / イオンチャンネル型センサ- / ボルタンメトリックセンサ- / ジカルボン酸陰イオンセンサ- / ATP^<4->センサ- / 分子認識素子 / 脂溶性大環状ポリアミン / サイクリックボルタンメトリ- / LangmuirーBlodgett(LB)膜型センサ- / フッ化物イオン / ジカルボン酸 / ヌクレオチド / 陰イオン選択性電極 / catalysis / model ligands / bleomycine / heme-like function / macrocyclic polyamine / superoxide dismutase / active oxygen species / activation of O_2 / SOD様活性 / 銅(II)錯体 / 大環状トリアミン / 1:1酸素付加体 / コバルト(II)錯体 / 大環状テトラアミン / 触媒 / モデル配位子 / ブレオマイシン / ヘム様機能 / 大環状ポリアミン配位子 / ス-パ-オキシドジスムタ-ゼ / 活性酸素種 / 酸素(O_2)の活性化 / mast cell / exocytosis / calcium channel / anti-allergic reaction / 育毛活性 / 発毛活性 / ヒスタミン遊離抑制 / ベンゾキノン / 好塩基球 / 好酸球 / 脱顆粒抑制 / カルシウムチャネル阻害 / 抗アレルギー作用 / micrphyllone / calcium chanell / basophils / mast cells / RBL-2H3 cells / Anti-allergics / 酸化還元電位 / ヒト好塩基球 / ラット腹腔マスト細胞 / RBL-2113細胞 / アレルギー / Gold Plating Agent / Nucleic Acid Recognition / CO_2 Fixation Catalyst / Anti-AIDS Drug / Zinc Enzyme / Artificial Enzyme / Intelligent Molecule / DNA高次構造変換 / アルコール脱水酵素 / 炭酸脱水酵素 / 蛋白合成阻害 / 核酸認識 / 炭酸ガス固定触媒 / 抗エイズ薬 / 人工酵素 / インテリジェント分子 / 金属イオンキレート剤 / 貴金属イオン / 高分子液膜型センサー / リン酸化プロテオーム / 質量分析 / 亜鉛錯体 / プロテオミクス / リン酸化ペプチド / リン酸化タンパク質 / 生体機能制御分子 / エネルギー移動 / 蛍光性金属錯体 / グアニン塩基 / 分子認識 / 核酸 / 生体分子 / 希土類錯体 / 芳香族スルホンアミド / ヒドリド転移触媒 / 炭酸脱水モデル / アルコール脱水酵素モデル / フェニルフォスフィンペンダントサイクラム / 金メッキ化剤 / 加水分解反応 / 金(III)イオン / 錯体化学 / 遷移金属イオン / 異常原子価 Less
  • Research Projects

    (26 results)
  • Research Products

    (106 results)
  • Co-Researchers

    (21 People)
  •  Screening of post-translational modified molecules by using functional zinc complexesPrincipal Investigator

    • Principal Investigator
      Koike Tohru
    • Project Period (FY)
      2020 – 2022
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Review Section
      Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
    • Research Institution
      Hiroshima University
  •  Fluorometric analysis of unstable phosphorylated biomoleculesPrincipal Investigator

    • Principal Investigator
      Koike Tohru
    • Project Period (FY)
      2017 – 2019
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Physical pharmacy
    • Research Institution
      Hiroshima University
  •  Analysis of phosphorylation signal using a Phos-tag quencherPrincipal Investigator

    • Principal Investigator
      Koike Tohru
    • Project Period (FY)
      2014 – 2016
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Physical pharmacy
    • Research Institution
      Hiroshima University
  •  Analytical methods for protein kinase profiling by using fluorescent Phos-tag moleculesPrincipal Investigator

    • Principal Investigator
      KOIKE Tohru
    • Project Period (FY)
      2010 – 2013
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      Physical pharmacy
    • Research Institution
      Hiroshima University
  •  Development of novel analytical methods for phosphoproteomics by using a phosphate-binding tag molecule.Principal Investigator

    • Principal Investigator
      KOIKE Tohru
    • Project Period (FY)
      2007 – 2009
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      Physical pharmacy
    • Research Institution
      Hiroshima University
  •  タンパク質リン酸化調節因子の迅速かつ簡便なスクリーニング法Principal Investigator

    • Principal Investigator
      小池 透
    • Project Period (FY)
      2006 – 2007
    • Research Category
      Grant-in-Aid for Exploratory Research
    • Research Field
      Drug development chemistry
    • Research Institution
      Hiroshima University
  •  Development of Analytical Procedures for the Phosphoproteome ResearchPrincipal Investigator

    • Principal Investigator
      KOIKE Tohru
    • Project Period (FY)
      2003 – 2006
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      Physical pharmacy
    • Research Institution
      Hiroshima University
  •  リン酸化タンパク質を捕捉する機能性分子の開発とプロテオーム研究への応用

    • Principal Investigator
      OHTANI Kazuhiro
    • Project Period (FY)
      2002 – 2003
    • Research Category
      Grant-in-Aid for Exploratory Research
    • Research Field
      分離・精製・検出法
    • Research Institution
      高知大学(医学部)
      Hiroshima University
  •  The development of new-type quinine derivatives regulating calcium channel

    • Principal Investigator
      OHTANI Kazuhiro
    • Project Period (FY)
      2002 – 2004
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      Bioorganic chemistry
    • Research Institution
      Kochi University
      高知大学(医学部)
      Hiroshima University
  •  活性型酵素への変換を目的とした人工分子シャペロンの開発Principal Investigator

    • Principal Investigator
      小池 透
    • Project Period (FY)
      2001 – 2003
    • Research Category
      Grant-in-Aid for Exploratory Research
    • Research Field
      医薬分子機能学
    • Research Institution
      Hiroshima University
  •  多核亜鉛錯体の自己集積による水溶性超分子の構築と均一系超分子触媒への展開Principal Investigator

    • Principal Investigator
      小池 透
    • Project Period (FY)
      2000
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas (A)
    • Review Section
      Science and Engineering
    • Research Institution
      Hiroshima University
  •  Development of novel artificial proteases and phosphatasesPrincipal Investigator

    • Principal Investigator
      KOIKE Tohru
    • Project Period (FY)
      2000 – 2002
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      医薬分子機能学
    • Research Institution
      HIROSHIMA UNIVERSITY
  •  Development of zinc detection system with novel zinc-fluorophoresPrincipal Investigator

    • Principal Investigator
      KOIKE Tohru
    • Project Period (FY)
      2000 – 2002
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      広領域
    • Research Institution
      HIROSHIMA UNIVERSITY
  •  Development of anti-allergics against antianaphylaxis type by lead-optimization

    • Principal Investigator
      OHTANI Kazuhiro
    • Project Period (FY)
      2000 – 2001
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Bioorganic chemistry
    • Research Institution
      HIROSHIMA UNIVERSITY
  •  A Basic Study on Artificial ProteasePrincipal Investigator

    • Principal Investigator
      KOIKE Tohru
    • Project Period (FY)
      1998 – 1999
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      医薬分子機能学
    • Research Institution
      Hiroshima University
  •  大環状ポリアミン希土類錯体による生体関連分子の認識および変換

    • Principal Investigator
      塩谷 光彦
    • Project Period (FY)
      1996
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas
    • Research Institution
      Okazaki National Research Institutes
  •  Synthesis of artificial phosphatases for the purpose of controling protein functionPrincipal Investigator

    • Principal Investigator
      KOIKE Tohru
    • Project Period (FY)
      1996 – 1997
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      医薬分子機能学
    • Research Institution
      HIROSHIMA UNIVERSITY
  •  大環状ポリアミン亜鉛錯体を用いた人工フォスファターゼの合成Principal Investigator

    • Principal Investigator
      小池 透
    • Project Period (FY)
      1995
    • Research Category
      Grant-in-Aid for General Scientific Research (C)
    • Research Field
      医薬分子機能学
    • Research Institution
      Hiroshima University
  •  人工亜鉛酵素を用いた新規含亜鉛ジペプチダーゼ阻害薬の開発Principal Investigator

    • Principal Investigator
      小池 透
    • Project Period (FY)
      1994
    • Research Category
      Grant-in-Aid for Encouragement of Young Scientists (A)
    • Research Field
      医薬分子機能学
    • Research Institution
      Hiroshima University
  •  リン酸エステル結合切断活性を有する人工リボヌクレアーゼの合成Principal Investigator

    • Principal Investigator
      小池 透
    • Project Period (FY)
      1993
    • Research Category
      Grant-in-Aid for Encouragement of Young Scientists (A)
    • Research Field
      医薬分子機能学
    • Research Institution
      Hiroshima University
  •  大環状配位子の特性に基づく異常原子価遷移金属イオンの化学

    • Principal Investigator
      木村 栄一
    • Project Period (FY)
      1992
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas
    • Research Institution
      Hiroshima University
  •  Creation of Intelligent and Robotic Molecules with Macrocyclic Polyamines

    • Principal Investigator
      KIMURA Eiichi
    • Project Period (FY)
      1992 – 1994
    • Research Category
      Grant-in-Aid for General Scientific Research (A)
    • Research Field
      Chemical pharmacy
    • Research Institution
      HIROSHIMA UNIVERSITY
  •  大環状配位子の特性に基づく異常原子価遷移金属イオンの化学

    • Principal Investigator
      KIMURA Eiichi
    • Project Period (FY)
      1991
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas
    • Research Institution
      Hiroshima University
  •  高分子歪み錯体系による動的解析と応用

    • Principal Investigator
      木村 栄一
    • Project Period (FY)
      1988
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas
    • Research Institution
      Okazaki National Research Institutes
  •  Design of Dioxygen Activating Agents and Active Oxygen Deactivating Agents and Their Medicinal Application

    • Principal Investigator
      KIMURA Eiichi
    • Project Period (FY)
      1988 – 1989
    • Research Category
      Grant-in-Aid for General Scientific Research (B)
    • Research Field
      Chemical pharmacy
    • Research Institution
      Coordination Chemistry Laboratories, Institute for Molecular Science
  •  Development of Novel Sensors Using Macrocyclic Polyamines as Sensory Elements

    • Principal Investigator
      UMEZAWA Yoshio
    • Project Period (FY)
      1988 – 1989
    • Research Category
      Grant-in-Aid for Developmental Scientific Research (B).
    • Research Field
      分析・地球化学
    • Research Institution
      Hokkaido University

All 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Other

All Journal Article Presentation Book Patent

  • [Book] Protein Modifications in Pathogenic Dysregulation of Signaling2015

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Total Pages
      15
    • Publisher
      Springer Japan
    • Data Source
      KAKENHI-PROJECT-26460036
  • [Book] Kinomics: Approaches and Applications2015

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Total Pages
      15
    • Publisher
      Wiley-VCH Verlag GmbH & Co. KGaA
    • Data Source
      KAKENHI-PROJECT-26460036
  • [Book] Phos-tag ゲルによるリン酸化タンパク質の分離・同定法,臨床プロテオーム(日本 プロテオーム研究会編)2012

    • Author(s)
      木下英司, 木下恵美子, 小池 透
    • Publisher
      金原出版
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Book] 蛍光性Phos-tag を用いたタンパク質リ ン酸化反応の解析法,生物物理化学, 日本電気泳動学会編2012

    • Author(s)
      木下英司, 木下恵美子, 多幾山敬, 宗村雅男, 小池 透
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Book] Phos-tag Affinity Electrophoresis for Protein Kinase Profiling "Protein Kinase Technologies, Neuromethods"2012

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, T. Koike
    • Publisher
      Humana Press, Springer
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Book] 細胞内リン酸化タンパク質を高精度に検出するためのPhos-tagビーズによるサンプル前処理法,細胞工学2009

    • Author(s)
      木下英司,木下恵美子,小池透
    • Total Pages
      938
    • Publisher
      秀潤社
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Book] Phosphate-affinity Gel Electrophoresis Using a Phos-tag Molecule for Phosphoproteome Study2009

    • Author(s)
      E.Kinoshita, E.Kinoshita-Kikuta, T.Koike
    • Total Pages
      104
    • Publisher
      Current Proteomics
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Book] 金属イオンの特性を利用したリン酸化タンパク質の特異的認識と検出2007

    • Author(s)
      木下英司,木下恵美子,小池透
    • Total Pages
      1897
    • Publisher
      薬学雑誌
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Book] リン酸基親和性クロマトグラフィーを用いた細胞内のリン酸化タンパク質の網羅的精製,バイオテクノロジージャーナル2007

    • Author(s)
      木下英司,木下恵美子,小池透
    • Total Pages
      217
    • Publisher
      羊土社
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Book] リン酸基アフィニティー電気泳動法を利用した遺伝子診断法,実験医学2007

    • Author(s)
      木下英司,木下恵美子,小池透
    • Total Pages
      3033
    • Publisher
      羊土社
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Book] 実験医学2006

    • Author(s)
      木下英司, 木下恵美子, 小池 透
    • Total Pages
      6
    • Publisher
      株式会社 羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] Characterization of Zinc(II) Complex of 1,4,7,10-Tetrazacyclododecane and Deprotonated 5-Fluorouracil (FU) in Crystalline/Solution States and Evaluation of Anticancer Activity: Approach for Improving the Anticancer Activity of FU2023

    • Author(s)
      Yoshimi Ichimaru, Koichi Kato, Rina Nakatani, Kirara Sugiura, Hideki Mizutani, Emiko Kinoshita-Kikuta, Tohru Koike, Wanchun Jin, Masanori Imai, Hiromasa Kurosaki
    • Journal Title

      Inorganic Chemistry Communications

      Volume: 147 Pages: 110221-110221

    • DOI

      10.1016/j.inoche.2022.110221

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-20K07210, KAKENHI-PROJECT-21K06455, KAKENHI-PROJECT-21K15244, KAKENHI-PROJECT-20K06988
  • [Journal Article] Phos-tag-based phosphate affinity chromatographic techniques2022

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      Journal of Chromatography Open

      Volume: 2 Pages: 10051-10061

    • DOI

      10.1016/j.jcoa.2022.100051

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-20K06988
  • [Journal Article] Use of <i>Escherichia coli</i> expression system for analyzing kinase motifs2022

    • Author(s)
      Kinoshita-Kikuta, E., Ino, Y., Kimura, Y., Akiyama, T., Kinoshita, E., Koike, T.
    • Journal Title

      J. Electrophor

      Volume: 66 Issue: 1 Pages: 13-20

    • DOI

      10.2198/jelectroph.66.13

    • ISSN
      1349-9394, 1349-9408
    • Language
      Japanese
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-21K06455, KAKENHI-PROJECT-20K06988
  • [Journal Article] Bis(nitrato-κO)(1,4,8,11-tetraazacyclotetradecane-κ4N)zinc(II) methanol monosolvate2022

    • Author(s)
      Ichimaru Yoshimi、Kato Koichi、Kurihara Masaaki、Jin Wanchun、Koike Tohru、Kurosaki Hiromasa
    • Journal Title

      IUCrData

      Volume: 7 Issue: 8 Pages: 220854-220863

    • DOI

      10.1107/s2414314622008549

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-21K06500, KAKENHI-PROJECT-21K15244, KAKENHI-PROJECT-17K08376, KAKENHI-PROJECT-20K06988
  • [Journal Article] Crystal Structure of [{<i>m,m</i>-bis(Zn<sup>II</sup>-cyclen)}<sub>3</sub>(μ<sub>2</sub>-CO<sub>3</sub>)<sub>2</sub>(H<sub>2</sub>O)<sub>2</sub>](ClO<sub>4</sub>)<sub>8</sub>·<i>n</i>H<sub>2</sub>O2022

    • Author(s)
      Y. Ichimaru, K. Kato, K. Sugiura, C. Hirata, W. Jin, H. Fujioka, H. Kurosaki, and T. Koike
    • Journal Title

      X-ray Structure Analysis Online

      Volume: 38 Issue: 0 Pages: 53-55

    • DOI

      10.2116/xraystruct.38.53

    • ISSN
      1883-3578
    • Year and Date
      2022-03-10
    • Language
      English
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-20K06988
  • [Journal Article] Crystal Structure of Bis{1,3-bis[bis(pyridin-2-ylmethyl)amino]propan-​2-olato-dizinc(II)}orthophosphate Tris(perchlorate) Octahydrate, [(Phos-tag)<sub>2</sub>-PO<sub>4</sub><sup>3−</sup>][ClO<sub>4</sub><sup>−</sup>]<sub>3</sub>·8H<sub>2</sub>O2021

    • Author(s)
      Ichimaru Yoshimi、Kato Koichi、Jin Wanchun、Sugiura Kirara、Kinoshita-Kikuta Emiko、KINOSHITA Eiji、Kurosaki Hiromasa、Koike Tohru
    • Journal Title

      X-ray Structure Analysis Online

      Volume: 37 Issue: 0 Pages: 87-88

    • DOI

      10.2116/xraystruct.37.87

    • NAID

      130008127785

    • ISSN
      1883-3578
    • Year and Date
      2021-12-10
    • Language
      English
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-17K08376, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-21K06455, KAKENHI-PROJECT-21K06500
  • [Journal Article] Crystal Structure of [1<sup>1</sup>,1<sup>4</sup>,1<sup>7</sup>,1<sup>10</sup>,5<sup>1</sup>,5<sup>4</sup>,5<sup>7</sup>,5<sup>10</sup>-Octaaza-1,5(1,4)-dicyclododecana-3,7(1,3)-dibenzenacyclooctaphane]zinc(II) tetrakis(nitrate), [<i>m,m</i>-bis(Zn<sup>II</sup>-cyclen)](NO<sub>3</sub>)<sub>4</sub>2021

    • Author(s)
      Y.Ichimaru, J. Wanchun, Y. Yamaguchi, K. Sugiura, M.Imai H. Kurosaki, H. Fujioka, Tohru Koike, Y. Hieda, and K. Kato
    • Journal Title

      X-ray Structure Analysis Online

      Volume: 37 Issue: 0 Pages: 15-16

    • DOI

      10.2116/xraystruct.37.15

    • NAID

      130007996645

    • ISSN
      1883-3578
    • Year and Date
      2021-03-10
    • Language
      English
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-20K06988
  • [Journal Article] Aqua(1,4,7,10-tetraazacyclodecane =cyclen)zinc(II) diperchlorate2021

    • Author(s)
      Y.Ichimaru, K. Kato, H. Kurosaki, H. Fujioka, M. Sakai, Y. Yamaguchi, J. Wanchun, K. Sugiura, M.Imai, and Tohru Koike
    • Journal Title

      IUCrData

      Volume: 6 Issue: 4 Pages: 397-415

    • DOI

      10.1107/s2414314621003977

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-20K06988
  • [Journal Article] Characterization of the binding of adenosine-5’-monophosphate to a mu-type alkoxide-linked dinuclear zinc(II) complex in crystal and solution state.2021

    • Author(s)
      Kinoshita-Kikuta, E., Ichimaru, Y., Yamano, Y., Kato, K., Kurosaki, H. Eiji Kinoshita, Koike, T.
    • Journal Title

      Bull. Chem. Soc. Jpn.

      Volume: 94(11) Issue: 11 Pages: 2670-2677

    • DOI

      10.1246/bcsj.20210280

    • NAID

      130008137574

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-21K06500
  • [Journal Article] Evaluation of four phosphopeptide enrichment strategies for mass spectrometry‐based proteomic analysis2021

    • Author(s)
      Ino Yoko、Kinoshita Eiji、Kinoshita‐Kikuta Emiko、Akiyama Tomoko、Nakai Yusuke、Nishino Kohei、Osada Makoto、Ryo Akihide、Hirano Hisashi、Koike Tohru、Kimura Yayoi
    • Journal Title

      PROTEOMICS

      Volume: 22 Issue: 7 Pages: 2100216-2100216

    • DOI

      10.1002/pmic.202100216

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19H03774, KAKENHI-PROJECT-20H03498, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-21K06455
  • [Journal Article] An assay of human tyrosine protein kinase ABL activity using an Escherichia coli protein expression system.2021

    • Author(s)
      Kinoshita-Kikuta E, Yoshimoto M, Yano M, Eiji Kinoshita, Koike T.
    • Journal Title

      Biotechniques

      Volume: 70(4) Issue: 4 Pages: 209-217

    • DOI

      10.2144/btn-2020-0154

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-21K06455
  • [Journal Article] Characterization of phosphorylation status and kinase activity of Src family kinases expressed in cell-based and cell-free protein expression system.2021

    • Author(s)
      Kinoshita-Kikuta E, Eiji Kinoshita, Suga M, Higashida M, Yamane Y, Nakamura T, Koike T.
    • Journal Title

      Biomolecules

      Volume: 11(10) Issue: 10 Pages: 1448-1448

    • DOI

      10.3390/biom11101448

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-21K06455
  • [Journal Article] A dot-blot-staining method for detecting phosphoproteins with a Phos-tag Aqua fluorescent dye2020

    • Author(s)
      Kinoshita-Kikuta Emiko、Akayama Keisuke、Kinoshita Eiji、Koike Tohru
    • Journal Title

      J. Electrophor

      Volume: 64 Issue: 1 Pages: 7-11

    • DOI

      10.2198/jelectroph.64.7

    • NAID

      130007961315

    • ISSN
      1349-9394, 1349-9408
    • Language
      English
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-18K06596
  • [Journal Article] Crystal Structure of [3-(1,4,7,10-Tetraazacyclododecan-1-yl)propan-1-amine]zinc(II) Bis(perchlorate)2020

    • Author(s)
      Y. Ichimaru, M. Imai, H. Fujioka, T. Koike, H. Kurosaki, and K. Kato
    • Journal Title

      X-ray Structure Analysis Online

      Volume: 36 Issue: 0 Pages: 43-44

    • DOI

      10.2116/xraystruct.36.43

    • NAID

      130007925228

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-20K06988
  • [Journal Article] Phos-tag-based micropipette-tip method for analysis of phosphomonoester-type impurities in synthetic oligonucleotides2020

    • Author(s)
      M. Tsunehiro, K. Sasaki, E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      Journal of Chromatography B

      Volume: 1151 Pages: 122198-122206

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-20K06988
  • [Journal Article] Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic2020

    • Author(s)
      Kinoshita-Kikuta Emiko、Utsumi Toshihiko、Miyazaki Aya、Tokumoto Chiharu、Doi Kyosuke、Harada Haruna、Kinoshita Eiji、Koike Tohru
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 16273-16273

    • DOI

      10.1038/s41598-020-73248-0

    • NAID

      120007169771

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K05827, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-18K06596
  • [Journal Article] An immuno-dot blot assay for screening histidine kinase inhibitors2020

    • Author(s)
      Kinoshita-Kikuta Emiko、Maruta Shino、Eguchi Yoko、Igarashi Masayuki、Okajima Toshihide、Utsumi Ryutaro、Kinoshita Eiji、Koike Tohru
    • Journal Title

      Analytical Biochemistry

      Volume: 600 Pages: 113765-113765

    • DOI

      10.1016/j.ab.2020.113765

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-18K06596
  • [Journal Article] A strategy to identify protein-N-myristoylation-dependent phosphorylation reactions of cellular proteins by using Phos-tag SDS-PAGE.2019

    • Author(s)
      Emiko Kinoshita-Kikuta, Ayane Tanikawa, Takuro Hosokawa, Aya Kiwado, Koko Moriya, Eiji Kinoshita, Tohru Koike, Toshihiko Utsumi
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 11 Pages: e0225510-e0225510

    • DOI

      10.1371/journal.pone.0225510

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-18K06596, KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-17K07758, KAKENHI-PROJECT-17K08237
  • [Journal Article] Increase in constitutively active MEK1 species by introduction of MEK1 mutations identified in cancers2019

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, S. Ueda, Y. Ino, Y. Kimura, H. Hirano, and T. Koike
    • Journal Title

      BBA - Proteins and Proteomics

      Volume: 1867 Issue: 1 Pages: 62-70

    • DOI

      10.1016/j.bbapap.2018.05.004

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-17K08237, KAKENHI-PROJECT-18K06596, KAKENHI-PROJECT-16H05230
  • [Journal Article] Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes2019

    • Author(s)
      Emiko Kinoshita-Kikuta, Hiroshi Kusamoto, Syogo Ono, Keisuke Akayama, Yoko Eguchi, Masayuki Igarashi, Toshihide Okajima, Ryutaro Utsumi, Eiji Kinoshita, Tohru Koike
    • Journal Title

      Electrophoresis

      Volume: 40 Issue: 22 Pages: 3005-3013

    • DOI

      10.1002/elps.201900261

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-18K06596, KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-17K08237
  • [Journal Article] Gel-based analysis of protein phosphorylation status by rapid fluorometric staining using TAMRA-labeled Phos-tag2019

    • Author(s)
      Hiroshi Kusamoto, Emiko Kinoshita-Kikuta, Tomoyo Nishimura, Tomomi Nagai, Eiji Kinoshita, and Tohru Koike,
    • Journal Title

      J. Electrophor

      Volume: 63 Issue: 1 Pages: 25-32

    • DOI

      10.2198/jelectroph.63.25

    • NAID

      130007634033

    • ISSN
      1349-9394, 1349-9408
    • Language
      English
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-18K06596, KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-17K08237
  • [Journal Article] A simple method for determining the ligand affinity toward a zinc-enzyme model by using a TAMRA/TAMRA interaction2018

    • Author(s)
      H. Kusamoto, A. Shiba, M. Tsunehiro, H. Fujioka, E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      Dalton Transactions

      Volume: 47 Issue: 6 Pages: 1841-1847

    • DOI

      10.1039/c7dt04364c

    • NAID

      120007018403

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17K08237
  • [Journal Article] Specific glutamic acid residues in targeted proteins induce exaggerated retardations in Phos-tag SDS-PAGE migration2017

    • Author(s)
      E.Kinoshita, E. Kinoshita-Kikuta, K. Karata, T. Kawano, A. Nishiyama, M. Yamato, and T. Koike
    • Journal Title

      Electrophoresis

      Volume: 38 Issue: 8 Pages: 1139-1146

    • DOI

      10.1002/elps.201600520

    • Peer Reviewed / Acknowledgement Compliant / Open Access
    • Data Source
      KAKENHI-PROJECT-26460036, KAKENHI-PROJECT-15K00541, KAKENHI-PROJECT-15K07887
  • [Journal Article] A Phos-tag-based micropipette-tip method for rapid and selective enrichment of phosphopeptides2017

    • Author(s)
      E. Tianfei Yuan, Y. Ino, M. Kawaguchi, Y. Kimura, H. Hirano, E. Kinoshita-Kikuta, E. Kinoshita, and Tohru Koike
    • Journal Title

      Electrophoresis

      Volume: 38 Issue: 19 Pages: 2447-2455

    • DOI

      10.1002/elps.201700175

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-17K08237, KAKENHI-PROJECT-16H05230
  • [Journal Article] TAMRA/TAMRA fluorescence quenching systems for the activity assay of alkaline phosphatase2017

    • Author(s)
      A. Shiba, E. Kinoshita-Kikuta, E. Kinoshita, and Tohru Koike
    • Journal Title

      Sensors

      Volume: 17 Issue: 8 Pages: 1877-1888

    • DOI

      10.3390/s17081877

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-17K08237, KAKENHI-PROJECT-15K07887
  • [Journal Article] A Phos-tag SDS-PAGE method that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK1.2016

    • Author(s)
      Kinoshita E, Kinoshita-Kikuta E, Kubota Y, Takekawa M and Koike T.
    • Journal Title

      Proteomics

      Volume: 16 Issue: 13 Pages: 1825-1836

    • DOI

      10.1002/pmic.201500494

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PUBLICLY-15H01205, KAKENHI-ORGANIZER-16H06573, KAKENHI-PROJECT-15H04703, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-26460036, KAKENHI-PROJECT-15K07887, KAKENHI-PROJECT-16K15212, KAKENHI-INTERNATIONAL-16K21739, KAKENHI-PLANNED-16H06574, KAKENHI-PROJECT-15K19019
  • [Journal Article] Expression and phosphorylation state analysis of intracellular protein kinases using Multi- PK antibody and Phos- tag SDS- PAGE.2015

    • Author(s)
      Sugiyama, Y., Katayama, S., Kameshita, I., Morisawa, K., Higuchi, T., Todaka, H., Eiji Kinoshita, Kinoshita- Kikuta, E., Koike, T., Taniguchi, T., Sakamoto, S.
    • Journal Title

      MethodsX

      Volume: 2 Pages: 469-474

    • DOI

      10.1016/j.mex.2015.11.007

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-15K07887, KAKENHI-PROJECT-26460036, KAKENHI-PROJECT-26750372, KAKENHI-PROJECT-14J07002, KAKENHI-PROJECT-25460371, KAKENHI-PROJECT-25560417, KAKENHI-PROJECT-25293005
  • [Journal Article] Identification of two phosphorylated species of β-catenin involved in the ubiquitin-proteasome pathway by using two-dimensional Phos-tag affinity electrophoresis2014

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      J. Electrophor

      Volume: 58 Issue: 1 Pages: 1-4

    • DOI

      10.2198/jelectroph.58.1

    • NAID

      130004713130

    • ISSN
      1349-9394, 1349-9408
    • Language
      English
    • Peer Reviewed / Acknowledgement Compliant / Open Access
    • Data Source
      KAKENHI-PROJECT-26460036
  • [Journal Article] Profiling of Protein Thiophosphorylation Using Phos-tag Affinity Electrophoresis: Evaluation of Adenosine 5′-O-(3-thiotriphosphate) as a Phosphoryl Donor in Protein Kinase Reactions2014

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, A. Shiba, K. Edahiro, Y. Inoue, K. Yamamoto, and T. Koike
    • Journal Title

      Proteomics

      Volume: 14 Issue: 6 Pages: 668-679

    • DOI

      10.1002/pmic.201300533

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417
  • [Journal Article] A Phos-tag-based fluorescence quenching system for activity assay and inhibitor screening for alkaline phosphatase2014

    • Author(s)
      Kinoshita-Kikuta, E., Kurosaki, H., Kunisada, N., Eiji Kinoshita, and Koike, T.
    • Journal Title

      Am. J. Anal. Chem.

      Volume: 5 Issue: 12 Pages: 796-804

    • DOI

      10.4236/ajac.2014.512088

    • Peer Reviewed / Acknowledgement Compliant / Open Access
    • Data Source
      KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417, KAKENHI-PROJECT-26460036
  • [Journal Article] A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules2013

    • Author(s)
      M. Tsunehiro, Y. Meki, K. Matsuoka, E. Kinoshita-Kikuta, E. Kinoshita and T. Koike
    • Journal Title

      Journal of Chromatography B

      Volume: 925 Pages: 86-94

    • DOI

      10.1016/j.jchromb.2013.02.039

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417, KAKENHI-PUBLICLY-23117522
  • [Journal Article] Phos-tag-Based Microarray Techniques Advance Phosphoproteomics2013

    • Author(s)
      Eiji Kinoshita, Emiko Kinoshita-Kikuta and Tohru Koike
    • Journal Title

      Journal of Proteomics & Bioinformatics

      Volume: S6 Issue: S6 Pages: 8-8

    • DOI

      10.4172/jpb.s6-008

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005
  • [Journal Article] Sandwich assay for phosphorylation of protein multiplexes by using antibodies and Phos-tag2013

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike
    • Journal Title

      Analytical Biochemistry

      Volume: 438 Issue: 2 Pages: 104-106

    • DOI

      10.1016/j.ab.2013.03.029

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417, KAKENHI-PUBLICLY-23117522
  • [Journal Article] Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions2012

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike
    • Journal Title

      Proteomics

      Volume: 12 Issue: 2 Pages: 192-202

    • DOI

      10.1002/pmic.201100524

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-22790037, KAKENHI-PUBLICLY-23117522
  • [Journal Article] Separation and identification of four distinct serine-phosphorylation states of ovalbumin by Phos-tag affinity electrophoresis2012

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      Electrophoresis

      Volume: 33 Issue: 5 Pages: 849-855

    • DOI

      10.1002/elps.201100518

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PUBLICLY-23117522
  • [Journal Article] Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin2012

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, Y. Sugiyama, Y. Fukada, T. Ozeki, and T. Koike
    • Journal Title

      Proteomics

      Volume: 12 Issue: 7 Pages: 932-937

    • DOI

      10.1002/pmic.201100639

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-23117522
  • [Journal Article] A Laborsaving, Timesaving, and More Reliable Strategy for Separation of Low-Molecular-Mass Phosphoproteins in Phos-tag Affinity Electrophoresis2012

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      International Journal of Chemistry

      Volume: 4 Issue: 5 Pages: 1-8

    • DOI

      10.5539/ijc.v4n5p1

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-23117522
  • [Journal Article] A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the kinase reaction of a substrate peptide2011

    • Author(s)
      M. Somura, K. Takiyama, E. Kinoshita- Kikuta, E. Kinoshita, and T. Koike
    • Journal Title

      Analytical Methods

      Volume: 3 Issue: 6 Pages: 1303-1309

    • DOI

      10.1039/c1ay05016h

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-22790037, KAKENHI-PUBLICLY-23117522
  • [Journal Article] A clean-up technology for the simultaneous determination of lysophosphatidic acid and sphingosine-1-phosphate by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry using a phosphate-capture molecule, Phos-tag2010

    • Author(s)
      J. Morishige, M. Urikura, H. Takagi, K. Hirano, T. Koike, T. Tanaka & K.
    • Journal Title

      Satouchi Rapid Commun. Mass Spectrom

      Volume: 24 Issue: 7 Pages: 1075-1084

    • DOI

      10.1002/rcm.4484

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21580141, KAKENHI-PROJECT-22390006
  • [Journal Article] The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1delta/ epsilon2010

    • Author(s)
      Sugiyama Y, Hatano N, Sueyoshi N, Suetake I, Tajima S, Kinoshita E, Kinoshita-Kikuta E, Koike T, and Kameshita I
    • Journal Title

      Biochem J

      Volume: 427 Issue: 3 Pages: 489-497

    • DOI

      10.1042/bj20091856

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21570136, KAKENHI-PROJECT-22370053, KAKENHI-PROJECT-22390006
  • [Journal Article] A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phospho-peptides2009

    • Author(s)
      K. Takiyama, E. Kinoshita, E. Kinoshita-Kikuta, Y. Fujioka, Y. Kubo, T. Koike
    • Journal Title

      Analytical Biochemistry 388

      Pages: 235-241

    • NAID

      120002023643

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE2009

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, T. Koike
    • Journal Title

      Nature Protocols 4

      Pages: 1513-1521

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] Two-dimensional phosphate affinity gel electrophoresis for the analysis of phosphoprotein isotypes2009

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, M. Matsubara, Y. Aoki, S. Ohie, Y. Mouri, T. Koike
    • Journal Title

      Electrophoresis 30

      Pages: 550-559

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] Phos-tag beads as an immunoblotting enhancer for selective detection of phosphoproteins in cell lysates2009

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, T. Koike
    • Journal Title

      Analytical Biochemistry 389

      Pages: 83-85

    • NAID

      120002023644

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] A mobility-shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gel electrophoresis2008

    • Author(s)
      E. Kinoshita-Kikuta, E. Kinoshita, T. Koike
    • Journal Title

      Analytical Biochemistry 378

      Pages: 102-104

    • NAID

      120001092444

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE2008

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, M. Matsubara, S. Yamada, H. Nakamura, Y. Shiro, Y. Aoki, K. Okita, T. Koike
    • Journal Title

      Proteomics 8

      Pages: 2994-3003

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway2008

    • Author(s)
      M. Ishiai, H. Kitao, A. Smogorzewska, J, Tomida, A. Kinomura, E. Uchida, A. Saberi, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, S. Tashiro, S.J. Elledge, M. Takata
    • Journal Title

      Nature Structural & Molecular Biology 15

      Pages: 1138-1146

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] Label-free kinase profiling using phosphate-affinity polyacrylamide gel electrophresis2007

    • Author(s)
      E.Kinoshita-Kikuta, Y.Aoki, E.Kinoshita, T.Koike
    • Journal Title

      Molecular & Cellular Proteomics 6

      Pages: 356-366

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] Separation of a phosphorylated histidine protein using phosphate affinity polyacrylamide gel electrophoresis2007

    • Author(s)
      S.Yamada, H.Nakamura, E.Kinoshita, E.Kinoshita-Kikuta, T.Koike, Y.Shiro
    • Journal Title

      Analytical Biochemistry 360

      Pages: 160-162

    • NAID

      120000878053

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] Separation of a phosphorylated-His protein using phosphate-affinity polyacrylamide gel2007

    • Author(s)
      S. Yamada, H. Nalkamura, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, Y. Shiro
    • Journal Title

      Analytical Biochemistry 360

      Pages: 160-162

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gel electrophoresis Analytical2007

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, T. Koike
    • Journal Title

      Biochemistry 361

      Pages: 294-298

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] Label-free kinase profiling using phosphate-affinity polyacrylamide gel electrophoresis2007

    • Author(s)
      E. Kinoshita-Kikuta, Y. Aoki, E. Kinoshita, T. Koike
    • Journal Title

      Molecular & Cellular Proteomics 6

      Pages: 356-366

    • NAID

      120000878140

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Journal Article] Phosphate-binding tag : A new tool to visualize phosphorylated proteins2006

    • Author(s)
      E.Kinoshita, E.Kinoshita-Kikuta, K.Takiyama, T.Koike
    • Journal Title

      Molecular & Cellular Proteomics 5

      Pages: 749-757

    • NAID

      120000878114

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] Enrichment of phosphorylated proteins from cell lysate using phosphate-affinity chromatography at physiological pH2006

    • Author(s)
      E.Kinoshita-Kikuta, E.Kinoshita, A.Yamada, M.Endo, T.Koike
    • Journal Title

      Proteomics 6

      Pages: 5088-5095

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] Detection and Quantification of On-Chip Phosphorylated Peptides by Surface Plasmon Resonance Imaging Techniques Using a Phosphate Capture Molecule2005

    • Author(s)
      K.Inamori, M.Kyo, Y.Nishiya, Y.Inoue, T.Sonoda, E.Kinoshita, T.Koike, Y.Katayama
    • Journal Title

      Analytical Chemistry 77

      Pages: 3979-3985

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] Novel immobilized zinc(II) affinity chromatography for phosphopeptides and phosphorylated proteins2005

    • Author(s)
      E.Kinoshita, A.Yamada, H.Takeda, E.Kinoshita-Kikuta, T.Koike
    • Journal Title

      Journal of Separation Science 28

      Pages: 155-162

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] An Alkoxide-bridged Dinuclear Zinc(II) Hexaazacryptate : a Novel Phosphate Capture Molecule in Aqueous Solution2005

    • Author(s)
      E.Kinoshita, K.Ishikawa, E.Kinoshita-Kikuta, K.Ohtani, M.Shiro, T.Koike
    • Journal Title

      Bulletin of the Chemical Society of Japan 78

      Pages: 125-131

    • NAID

      10014271953

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] Quantitative analysis of lysophosphatidic acid by time-of-flight mass spectrometry using a phosphate capture molecule2004

    • Author(s)
      T.Tanaka, H.Tsutsui, K.Hirano, T.Koike, A.Tokumura, K.Satouchi
    • Journal Title

      Journal of Lipid Research 45

      Pages: 2145-2150

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] Recognition of phosphate monoester dianion by an alkoxide-bridged dinuclear zinc(II) complex2004

    • Author(s)
      E.Kinoshita, M.Takahashi, H.Takeda, M.Shiro, T.Koike
    • Journal Title

      Dalton Transactions

      Pages: 1189-1193

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Journal Article] Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of phosphorylated compounds using a novel phosphate capture molecule2003

    • Author(s)
      H.Takeda, A.Kawasaki, M.Takahashi, A.Yamada, T.Koike
    • Journal Title

      Rapid Communication of Mass Spectrometry 17

      Pages: 2075-2081

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Patent] リン酸基を有する物質の染色方法2005

    • Inventor(s)
      小池 透
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Number
      2005-230583
    • Filing Date
      2005-08-09
    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-15390013
  • [Presentation] リン酸化タンパク質特異的ゲル蛍光染色2018

    • Author(s)
      草本 寛,西村 朋世,木下 恵美子,木下 英司,小池 透
    • Organizer
      日本電位泳動学会総会
    • Data Source
      KAKENHI-PROJECT-17K08237
  • [Presentation] 亜鉛酵素モデルに対するリガンドの親和性を解析する新規分光分析法の開発2018

    • Author(s)
      草本 寛,芝 晃生,常弘昌弥,藤岡晴人,木下英司, 木下恵美子, 小池 透
    • Organizer
      日本薬学会第138年会
    • Data Source
      KAKENHI-PROJECT-17K08237
  • [Presentation] Phosphorylated protein specific fluorescent gel stain using a TAMRA-Phos-tag2018

    • Author(s)
      草本 寛,木下 恵美子,木下 英司,小池 透
    • Organizer
      日本生化学会
    • Data Source
      KAKENHI-PROJECT-17K08237
  • [Presentation] 蛍光クエンチングを用いるアルカリホスファターゼ分析の新規蛍光分析法の開発2018

    • Author(s)
      芝 晃生,木下英司, 木下恵美子, 小池 透
    • Organizer
      日本薬学会第138年会
    • Data Source
      KAKENHI-PROJECT-17K08237
  • [Presentation] 金属配位結合を利用したプロテオミクス2017

    • Author(s)
      小池 透
    • Organizer
      日本電気泳動学会第68回総会
    • Invited
    • Data Source
      KAKENHI-PROJECT-17K08237
  • [Presentation] 蛍光クエンチングを用いる脱リン酸化酵素活性の新規蛍光分析法の開発2017

    • Author(s)
      芝 晃生,木下恵美子,木下英司,小池 透
    • Organizer
      日本電気泳動学会第68回総会
    • Data Source
      KAKENHI-PROJECT-17K08237
  • [Presentation] フォスタグ技術を用いたヒスチジンキナーゼにおける自己リン酸化ダイナミクスのプロファイリング2016

    • Author(s)
      木下英司,木下恵美子,小池 透
    • Organizer
      日本プロテオーム学会2016年大会
    • Place of Presentation
      東京都
    • Year and Date
      2016-07-28
    • Data Source
      KAKENHI-PROJECT-26460036
  • [Presentation] Phos-tag SDS-PAGE methodology that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK12016

    • Author(s)
      Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
    • Organizer
      HUPO2016 台湾大会
    • Place of Presentation
      Taipei (Taiwan)
    • Year and Date
      2016-09-18
    • Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-26460036
  • [Presentation] 大腸菌ハイブリッドセンサーキナーゼにおけるリン酸基転移反応様式の検証2016

    • Author(s)
      木下英司,木下恵美子,江口陽子,小池 透
    • Organizer
      日本生化学会第89回大会
    • Place of Presentation
      仙台市
    • Year and Date
      2016-09-25
    • Data Source
      KAKENHI-PROJECT-26460036
  • [Presentation] Development of Thio-tag magnetic bead for rapid and selective separation of thiol-containing molecules2015

    • Author(s)
      Maho Kawaguchi, Norinao Koretake, Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
    • Organizer
      環太平洋国際化学会議 PACIFICHEM 2015
    • Place of Presentation
      米国 ホノルル市
    • Year and Date
      2015-12-15
    • Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-26460036
  • [Presentation] リン酸基捕捉能を持つダーククエンチャーを用いた蛍光分析法2015

    • Author(s)
      國貞夏実,木下恵美子,木下英司,小池 透
    • Organizer
      第66回日本電気泳動学会総会
    • Place of Presentation
      東京都
    • Year and Date
      2015-09-04
    • Data Source
      KAKENHI-PROJECT-26460036
  • [Presentation] Phos-tag Biotin as an on-demand tool for study on protein phosphorylome2013

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike
    • Organizer
      12th HUPO Annual World Congress
    • Place of Presentation
      Yokohama
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] 迅速かつ簡便にリン酸化生体分子を分離精製するためのPhos-tag磁気ビーズの開発2013

    • Author(s)
      常弘昌弥, 目木勇馬, 木下恵美子, 木下 英司, 小池 透
    • Organizer
      第64回日本電気泳動学会
    • Place of Presentation
      仙台
    • Year and Date
      2013-11-15
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] Phos-tag Biotinの開発とリン酸化プロ テオミクスへの応用2012

    • Author(s)
      木下英司, 木下恵美子, 小池 透
    • Organizer
      10th JHUPO
    • Place of Presentation
      東京
    • Year and Date
      2012-07-27
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] 中性 phos-tag PAGEを用いたタンパク質リン酸化反応の解析2012

    • Author(s)
      木下英司, 木下恵美子, 小池 透
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-15
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] Phos-tag SDS-PAGE systems for protein phosphorylation profiling under neutral pH2012

    • Author(s)
      E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike
    • Organizer
      11th HUPO Annual World Congress
    • Place of Presentation
      Boston, USA
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] リン酸基結合タグ分子(Phos-tag)を用いたリン酸化生体分子の解析法2011

    • Author(s)
      小池透
    • Organizer
      日本化学会生体関連化学部会サマースクール
    • Place of Presentation
      広島県廿日市市(招待講演)
    • Year and Date
      2011-07-23
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] リン酸基結合タグ分子(Phos-tag)を用いたリン酸化生体分子の解析法2011

    • Author(s)
      小池 透
    • Organizer
      生体機能関連化学部会 若手の会 第23回サマースクール
    • Place of Presentation
      廿日市市
    • Year and Date
      2011-07-23
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] 改良型Phos-tag Biotinを用いたペプチドマイクロアレイ解析によるリン酸化反応プロファイリング2011

    • Author(s)
      木下英司, 木下恵美子, 小池 透
    • Organizer
      9th JHUPO
    • Place of Presentation
      新潟
    • Year and Date
      2011-07-28
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] Phos-tag fluorescence resonance energy transfer system for the analysis of phosphorylated molecules2010

    • Author(s)
      M. Somura, K. Takiyama, T. Nagai, E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike
    • Organizer
      International Chemical Congress of Pacific Basin
    • Place of Presentation
      Honolulu, USA
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] リン酸基親和性電気泳動法を用いたヒスチジン・アスパラギン酸リン酸化タンパク質の定量解析法2009

    • Author(s)
      柳原志穂, 木下恵美子, 木下英司, 中神明, 小池透
    • Organizer
      第82回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-21
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] Phos-tag固定化ポリマーを利用したシグナル伝達解析における選択的蛋白質リン酸化検出のための新技術2009

    • Author(s)
      木下英司, 木下恵美子, 小池透
    • Organizer
      日本ヒトプロテオーム機構第7回大会
    • Place of Presentation
      東京
    • Year and Date
      2009-07-27
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] 蛋白質リン酸化解析のためのリン酸基親和性2次元電気泳動法2009

    • Author(s)
      木下英司, 木下恵美子, 小池透
    • Organizer
      第60回日本電気泳動学会総会
    • Place of Presentation
      長野
    • Year and Date
      2009-09-18
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] フォスタグ結合型新規リン酸基アフィニティービーズを利用した細胞抽出液からのリン酸化蛋白質の分離と精製2008

    • Author(s)
      木下英司, 木下恵美子, 山田篤志, 井上知香, 小池透
    • Organizer
      日本ヒトプロテオーム機構第6回大会
    • Place of Presentation
      大阪
    • Year and Date
      2008-07-29
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] ウエスタンブロッティング膜に転写されたリン酸化蛋白質の検出とダイレクト膜上質量分析2008

    • Author(s)
      木下英司, 木下恵美子, 中西豪, 安藤英治, 古田大, 綱澤進, 西村紀, 小池透
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Year and Date
      2008-03-26
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] リン酸基結合ナノ分子を利用した新しい二次元電気泳動法の開発2007

    • Author(s)
      木下英司, 木下恵美子, 大家志織, 松原守, 小池透
    • Organizer
      第49回生化学会中四国支部例会
    • Place of Presentation
      高知
    • Year and Date
      2007-05-19
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] リン酸化プロテオーム研究の新戦略2007

    • Author(s)
      木下英司, 木下恵美子, 小池透
    • Organizer
      日本ヒトプロテオーム機構第5回大会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-30
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] 消化プロセスで生じる創傷治癒ホルモン・リゾホスファチジン酸による消化管組織修復2007

    • Author(s)
      中保, 堀内剛, 平野薫, 徳村彰, 盛重純一, 小池透, Mandi Murph, Gordon Mills
    • Organizer
      里内清, 第49回生化学会中四国支部例会
    • Place of Presentation
      高知
    • Year and Date
      2007-05-19
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] Phosphate affinity 2-D gel electro- phoresis for the analysis of phospho- proteins2007

    • Author(s)
      E. Kinoshita, E. Kinoshita- Kikuta, Y. Aoki, S. Ohie, Y. Mouri, Mamoru Matsubara, T. Koike
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-12
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] 遺伝子変異を認識する機能性分子を用いた遺伝子診断技術の開発2007

    • Author(s)
      木下英司, 木下恵美子, 由元実希, 小池透
    • Organizer
      第58回日本電気泳動学会総会
    • Place of Presentation
      宇部
    • Year and Date
      2007-11-07
    • Data Source
      KAKENHI-PROJECT-19390011
  • [Presentation] Quantitative analysis of effects of pH on the stability of His- and Asp-phosphoproteins in bacterial two-component system by using phosphate-affinity SDS-PAGE

    • Author(s)
      Akio Shiba, Keisuke Edahiro, Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
    • Organizer
      12th HUPO World Congress
    • Place of Presentation
      横浜市
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] 迅速かつ簡便にリン酸化生体分子を分離精製するためのPhos-tag磁気ビーズの開発

    • Author(s)
      常弘 昌弥, 目木 勇馬, 木下 恵美子,木下 英司,小池 透
    • Organizer
      日本電気泳動学会
    • Place of Presentation
      仙台市
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] Quantitative analysis of the phosphorylation state of a hybrid histidine kinase in bacterial two-component system by using phosphate-affinity SDS-PAGE

    • Author(s)
      Keisuke Edahiro, Akio Shiba, Emiko Kinoshita-Kikuta, Yuki Inoue, Eiji Kinoshita, and Tohru Koike
    • Organizer
      12th HUPO World Congress
    • Place of Presentation
      横浜市
    • Data Source
      KAKENHI-PROJECT-22390006
  • [Presentation] 近接Phos-tagゲルを用いたリン酸化タンパク質の電気泳動法

    • Author(s)
      目木 勇馬,木下 英司,木下 恵美子, 小池 透
    • Organizer
      日本電気泳動学会
    • Place of Presentation
      仙台市
    • Data Source
      KAKENHI-PROJECT-22390006
  • 1.  KIMURA Eiichi (30034010)
    # of Collaborated Projects: 6 results
    # of Collaborated Products: 0 results
  • 2.  SHIONOYA Mitsuhiko (60187333)
    # of Collaborated Projects: 6 results
    # of Collaborated Products: 0 results
  • 3.  OHTANI Kazuhiro (20203820)
    # of Collaborated Projects: 6 results
    # of Collaborated Products: 0 results
  • 4.  KUMAGAI Takanori (70274058)
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 0 results
  • 5.  TOMINAGA Akira (50172193)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 6.  KINOSHITA Eiji (80304418)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 14 results
  • 7.  UMEZAWA Yoshio (80011724)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 8.  KATAOKA Masamitsu (40091425)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 9.  SUGAWARA Masao (50002176)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 10.  ODASHIMA Kazunori (30152507)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 11.  青木 伸 (00222472)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 12.  杉山 政則 (30106801)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 13.  秀 道弘 (50284188)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 14.  武川 睦寛
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 15.  木下 恵美子
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 4 results
  • 16.  水谷 秀樹
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 17.  田中 保
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 18.  木村 弥生
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 19.  黒崎 博雅
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 20.  江口 陽子
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 21.  末武 勲
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results

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