HARA Toshiaki 原利明

Researcher Number	90528233
Other IDs
Affiliation (based on the past Project Information) *help	2013: 大阪大学, 理学(系)研究科(研究院), 特任講師(常勤) 2012: 大阪大学, 理学(系)研究科(研究院), 講師 2011: 大阪大学, 大学院・理学研究科, 特任講師 2011: 大阪大学, 理学研究科, 特任講師 2010: 大阪大学, 蛋白質研究所, 助教
Review Section/Research Field	Principal Investigator Living organism molecular science
Keywords	Principal Investigator ペプチド化学 / 脂質膜 / 光分解リンカー / 膜蛋白質 / 構造生物学 / ペプチド化学合成 / 膜タンパク質 / 天然物有機化学

Research Projects
(2 results)
Research Products
(33 results)

Folding of cysteine-rich peptides on phospholipid membranePrincipal Investigator
- Principal Investigator
  
  HARA Toshiaki
- Project Period (FY)
  2012 – 2013
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Living organism molecular science
- Research Institution
  Osaka University
Development of a method for purification of membrane proteins by photocleavable linkers.Principal Investigator
- Principal Investigator
  
  HARA Toshiaki
- Project Period (FY)
  2010 – 2011
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Living organism molecular science
- Research Institution
  Osaka University

All 2014 2013 2012 2011 2010

All Journal Article Presentation Book

[Book] タンパク質結晶の最前線　第 II 編2013
- Author(s)
  原利明 (杉山成編)
- Total Pages
  7
- Publisher
  シーエムシー出版
- Data Source
  KAKENHI-PROJECT-24651242
[Book] 原利明「化学合成ペプチド・タンパク質の調製と結晶構造解析」、杉山成編『タンパク質結晶の最前線』第II編2013
- Author(s)
  原利明
- Publisher
  シーエムシー出版
- Data Source
  KAKENHI-PROJECT-24651242
[Journal Article] Purification of Transmembrane Peptides using RP-HPLC.2014
- Author(s)
  Huang, Y.; Hara, T.; Murata M.
- Journal Title
  
  Peptides Science 2013
  
  Volume: 未定
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651242
[Journal Article] Folding of cysteine-rich peptides on phospholipid membranes.2014
- Author(s)
  Hara1, T.; Purwati, E.M.; Wada, A.; Hasegawa, M.; Murata, M.; Aimoto, S.
- Journal Title
  
  Peptides Science 2013
  
  Volume: 未定
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651242
[Journal Article] Differential Effects of Peptides Stomagen, EPF1, and EPF2 on Activation of the MAP Kinase MPK6 and the SPCH Protein Level2013
- Author(s)
  Pawan, K. J., Hara, T., Tanaka, H., Kondo, T., Betsuyaku, S., Sawa, S., Sakagami, Y., Aimoto, S., and Kakimoto, T.
- Journal Title
  
  Plant Cell Physiol
  
  Volume: 54 Pages: 1253-1262
- NAID
  10031195179
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651242
[Journal Article] Differential Effects of Peptides Stomagen, EPF1, and EPF2 on Activation of the MAP Kinase MPK6 and the SPCH Protein Level.2013
- Author(s)
  Pawan, K. J., Hara, T., Tanaka, H., Kondo, T., Betsuyaku, S., Sawa, S., Sakagami, Y., Aimoto, S., and Kakimoto, T.
- Journal Title
  
  Plant Cell Physiol.
  
  Volume: 54 Pages: 1253-1262
- NAID
  10031195179
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651242
[Journal Article] Purification of Transmembrane Peptides using RP-HPLC., Ed. Teshima T. and Nishiushi Y2013
- Author(s)
  Huang, Y.; Hara, T.; Murata M.
- Journal Title
  
  Peptides Science 2013
  
  Volume: (in press)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651242
[Journal Article] Folding of cysteine-rich peptides on phospholipid membranes2013
- Author(s)
  Hara1, T.; Purwati, E.M.; Wada, A.; Hasegawa, M.; Murata, M.; Aimoto, S.
- Journal Title
  
  Peptides Science 2013
  
  Volume: (in press)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651242
[Journal Article] Structure of the human heart fatty acid-binding protein in complex with the fluorescent probe 1-anilinonaphtalene-8- sulphonic acid.2013
- Author(s)
  Hirose, M., Sugiyama, S., Ishida, H., Niiyama, M., Matsuoka, D., Hara, T., Mizohata, E., Murakami, S., Inoue, T., Matsuoka, S. and Murata, M.
- Journal Title
  
  Journal of Synchrotron Radiation
  
  Volume: 20 Pages: 923-928
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651242
[Journal Article] Structure of the human heart fatty acid-binding protein in complex with the fluorescent probe 1-anilinonaphtalene-8-sulphonic acid2013
- Author(s)
  Hirose, M., Sugiyama, S., Ishida, H., Niiyama, M., Matsuoka, D., Hara, T., Mizohata, E., Murakami, S., Inoue, T., Matsuoka, S. and Murata, M.
- Journal Title
  
  Journal of Synchrotron Radiation
  
  Volume: 20 Pages: 923-928
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24651242
[Journal Article] New Redox Linker For The Preparation of Membrane Protein "Subunitc of Human FoF_1-ATP Synthase2011
- Author(s)
  Hara, T.; Yajima, N.; Aimoto, S.
- Journal Title
  
  Peptides
  
  Volume: 2010 Pages: 199-200
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22710216
[Journal Article] Chemical Synthesis of the Functional Domain of Voltage-Gated Proton Channel2010
- Author(s)
  Nakamura, K.; Hara, T.; Akai, Y.; Sato, T.; Kawakami, T.; Aimoto, S.
- Journal Title
  
  Peptides
- NAID
  10028237752
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22710216
[Journal Article] Synthetic studies of membrane proteins : the subunit c of human F1Fo-ATP synthase and voltage-gated proton channel(78-222). Ed. Rui Wang and Kai-Rong Wang.2010
- Author(s)
  Saburo Aimoto, Ken' ichiro Nakamura, Takeshi Sato, Toru Kawakami, and Toshiaki Hara
- Journal Title
  
  Peptides, 11^<th> CPS2010
  
  Pages: 47-53
- Data Source
  KAKENHI-PROJECT-22710216
[Journal Article] New Redox Linker For The Preparation of Membrane Protein" Subunit c of Human F_OF_1-ATP Synthase"2010
- Author(s)
  Hara, T.; Yajima, N.; Aimoto, S.
- Journal Title
  
  Peptides
  
  Pages: 199-199
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22710216
[Presentation] Novel fatty acid analogues for the understanding of lipid-protein interactions2013
- Author(s)
  Hanako Ishida, Shigeru Sugiyama, Mika Hirose, Sebastien Lethu, Hikaru Ano, Fuminori Sato, Daisuke Matsuoka, Toshiaki Hara, Eiichi Mizohata, Tsuyoshi Inoue, Shigeru Matsuoka, Michio Murata
- Organizer
  The International Conference on Structural Genomics 2013 (ICSG2013)第7回国際構造ゲノム会議
- Place of Presentation
  北海道(札幌)(ポスター発表)
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] ヒト心筋型脂肪酸結合タンパク質FABP3と蛍光プローブ1-anilinonaphtalene-8-sulphonic acidの複合体結晶構造解析2013
- Author(s)
  廣瀬未果、松岡茂・石田英子・松岳大輔・原利明・溝端栄一・村上聡・井上豪・村田道雄・杉山成
- Organizer
  日本結晶学会平成25年度年会
- Place of Presentation
  熊本(ポスター発表)
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] Folding of cysteine-rich peptides on phospholipid membranes2013
- Author(s)
  Hara, T.; Purwati, E.M.; Wada, A.; Hasegawa, M.; Murata, M.; Aimoto, S.
- Organizer
  4th Asia-Pacific International Peptide Symposium & 50th Japanese Peptide Symposium
- Place of Presentation
  大阪(ポスター発表)
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] Novel fatty acid analogues for the understanding of lipid-protein interactions.2013
- Author(s)
  Hanako Ishida, Shigeru Sugiyama, Mika Hirose, Sebastien Lethu, Hikaru Ano, Fuminori Sato, Daisuke Matsuoka, Toshiaki Hara, Eiichi Mizohata, Tsuyoshi Inoue, Shigeru Matsuoka, Michio Murata.
- Organizer
  he International Conference on Structural Genomics 2013 (ICSG2013)
- Place of Presentation
  札幌
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] Purification of Transmembrane Peptides using RP-HPLC.2013
- Author(s)
  Huang, Y.; Hara, T.; Murata M.
- Organizer
  4th Asia-Pacific International Peptide Symposium & 50th Japanese Peptide Symposium
- Place of Presentation
  吹田市
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] Structure of the human muscle fatty acid-binding protein complexed with competitive inhibitor2013
- Author(s)
  Mika Hirose, Shigeru Sugiyama, Hanako Ishida, Daisuke Matsuoka, Toshiaki Hara, Eiichi Mizohata, Tsuyoshi Inoue, Shigeru Matsuoka, Michio Murata
- Organizer
  The International Conference on Structural Genomics 2013 (ICSG2013)第7回国際構造ゲノム会議
- Place of Presentation
  北海道(札幌)(ポスター発表)
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] Folding of cysteine-rich peptides on phospholipid membranes.2013
- Author(s)
  Hara1, T.; Purwati, E.M.; Wada, A.; Hasegawa, M.; Murata, M.; Aimoto, S.
- Organizer
  4th Asia-Pacific International Peptide Symposium & 50th Japanese Peptide Symposium
- Place of Presentation
  吹田市
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] Purification of Transmembrane Peptides using RP-HPLC2013
- Author(s)
  Huang, Y.; Hara, T.; Murata M.
- Organizer
  4th Asia-Pacific International Peptide Symposium & 50th Japanese Peptide Symposium
- Place of Presentation
  大阪(ポスター発表)
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] ヒト心筋型脂肪酸結合タンパク質FABP3と蛍光プローブ1-anilinonaphtalene-8-sulphonic acidの複合体結晶構造解析2013
- Author(s)
  廣瀬未果、松岡茂・石田英子・松岳大輔・原利明・溝端栄一・村上聡・井上豪・村田道雄・杉山成、
- Organizer
  日本結晶学会　平成25年度年会
- Place of Presentation
  熊本市
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] システインリッチペプチドの脂質膜上での酸化的フォールディング反応2013
- Author(s)
  原利明、Euis Maras Purwati 、和田昭裕、長谷川慎、村田道雄、相本三郎
- Organizer
  第13回日本蛋白質科学会年会
- Place of Presentation
  鳥取
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] Structure of the human muscle fatty acid-binding protein complexed with Hydrophobic ligand2013
- Author(s)
  Shigeru Sugiyama, Hanako Ishida, Daisuke Matsuoka, Toshiaki Hara, Eiichi Mizohata, Tsuyoshi Inoue, Shigeru Matsuoka, Michio Murata
- Organizer
  4th International Symposium on Diffraction Structural Biology(第4回回折構造生物国際シンポジウム2013)
- Place of Presentation
  (ポスター発表)
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] 脂質膜を用いるシステインリッチペプチドのフォールディング反応2012
- Author(s)
  原利明、Euis Maras Purwati 、和田昭裕、長谷川慎、村田道雄、相本三郎
- Organizer
  第７回日本ケミカルバイオロジー学会年回
- Place of Presentation
  京都
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] システインリッチペプチドの脂質膜上での酸化的フォールディング反応2012
- Author(s)
  原利明、エイスマラスプルワティ、和田昭裕、長谷川慎、村田道雄、相本三郎
- Organizer
  第13回日本蛋白質科学会年会
- Place of Presentation
  鳥取(ポスター発表)
- Data Source
  KAKENHI-PROJECT-24651242
[Presentation] Membrane-induced folding reaction of disulfide-rich peptides2011
- Author(s)
  Toshiaki HARA
- Organizer
  International ERATO Symposium on Lipid Structures in and around Proteins
- Place of Presentation
  大阪、吹田
- Year and Date
  2011-11-14
- Data Source
  KAKENHI-PROJECT-22710216
[Presentation]2011
- Author(s)
  原利明, 矢嶋奈央, Euis Maras Purwati, 相本三郎
- Organizer
  日本化学会第91回春季年会
- Place of Presentation
  神奈川、横浜、口頭発表
- Year and Date
  2011-03-11
- Data Source
  KAKENHI-PROJECT-22710216
[Presentation] "New linker system for the preparation of membrane proteins." IPR Seminar2010
- Author(s)
  Toshiaki Hara
- Organizer
  The New Trend of Peptide Science in Asia and Oceania
- Place of Presentation
  大阪、吹田、口頭発表
- Year and Date
  2010-10-10
- Data Source
  KAKENHI-PROJECT-22710216
[Presentation] New Redox Linker For The Preparation Of Membrane Protein : Subunit c of Human F_1F_o-ATP Synthase2010
- Author(s)
  Toshiaki Hara, Nao Yajima, and Saburo Aimoto
- Organizer
  The 5th International Peptide Symposium
- Place of Presentation
  京都、ポスター発表
- Year and Date
  2010-10-09
- Data Source
  KAKENHI-PROJECT-22710216
[Presentation] the subunit c of human F1Fo-ATP synthase and voltage-gated proton channel(78-222)2010
- Author(s)
  Saburo Aimoto*, Ken' ichiro Nakamura, Takeshi Sato, Toru Kawakami, and Toshiaki Hara
- Organizer
  Synthetic studies of membrane proteins
- Place of Presentation
  中国、蘭州、口頭発表
- Data Source
  KAKENHI-PROJECT-22710216
[Presentation] Chemical Synthesis of the Functional Domain of Voltage-Gated Proton Channel2010
- Author(s)
  Ken' ichiroh Nakamura, Toshiaki Hara, Yuichi Akai, Takeshi Sato, Toru Kawakami, Saburo Aimoto
- Organizer
  The 5th International Peptide Symposium
- Place of Presentation
  京都、ポスター発表
- Year and Date
  2010-10-09
- Data Source
  KAKENHI-PROJECT-22710216

HARA Toshiaki 原 利明

Research Projects

Research Products

Folding of cysteine-rich peptides on phospholipid membranePrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of a method for purification of membrane proteins by photocleavable linkers.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Book] タンパク質結晶の最前線 第 II 編2013

Author(s)

Total Pages

Publisher

Data Source

[Book] 原利明「化学合成ペプチド・タンパク質の調製と結晶構造解析」、杉山成編『タンパク質結晶の最前線』第II編2013

Author(s)

Publisher

Data Source

[Journal Article] Purification of Transmembrane Peptides using RP-HPLC.2014

Author(s)

Journal Title

Data Source

[Journal Article] Folding of cysteine-rich peptides on phospholipid membranes.2014

Author(s)

Journal Title

Data Source

[Journal Article] Differential Effects of Peptides Stomagen, EPF1, and EPF2 on Activation of the MAP Kinase MPK6 and the SPCH Protein Level2013

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Differential Effects of Peptides Stomagen, EPF1, and EPF2 on Activation of the MAP Kinase MPK6 and the SPCH Protein Level.2013

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Purification of Transmembrane Peptides using RP-HPLC., Ed. Teshima T. and Nishiushi Y2013

Author(s)

Journal Title

Data Source

[Journal Article] Folding of cysteine-rich peptides on phospholipid membranes2013

Author(s)

Journal Title

Data Source

[Journal Article] Structure of the human heart fatty acid-binding protein in complex with the fluorescent probe 1-anilinonaphtalene-8- sulphonic acid.2013

Author(s)

Journal Title

Data Source

[Journal Article] Structure of the human heart fatty acid-binding protein in complex with the fluorescent probe 1-anilinonaphtalene-8-sulphonic acid2013

Author(s)

Journal Title

Data Source

[Journal Article] New Redox Linker For The Preparation of Membrane Protein "Subunitc of Human FoF_1-ATP Synthase2011

Author(s)

Journal Title

Data Source

[Journal Article] Chemical Synthesis of the Functional Domain of Voltage-Gated Proton Channel2010

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Synthetic studies of membrane proteins : the subunit c of human F1Fo-ATP synthase and voltage-gated proton channel(78-222). Ed. Rui Wang and Kai-Rong Wang.2010

Author(s)

Journal Title

Data Source

[Journal Article] New Redox Linker For The Preparation of Membrane Protein" Subunit c of Human F_OF_1-ATP Synthase"2010

Author(s)

Journal Title

Data Source

[Presentation] Novel fatty acid analogues for the understanding of lipid-protein interactions2013

Author(s)

Organizer

Place of Presentation

Data Source

HARA Toshiaki 原利明

[Book] タンパク質結晶の最前線　第 II 編2013