Miura Hiromi 三浦浩美

… Alternative Names

Researcher Number	90599523
Affiliation (Current)	2020: 東海大学, 医学部, 特任助教
Affiliation (based on the past Project Information) *help	2020: 東海大学, 医学部, 特任助教 2016 – 2018: 東海大学, 医学部, 特定研究員 2013 – 2014: 東海大学, 医学部, 特定研究員 2012: 東海大学, 医学部, その他
Review Section/Research Field	Principal Investigator Laboratory animal science / Integrative animal science / Basic Section 42040:Laboratory animal science-related Except Principal Investigator Laboratory animal science
Keywords	Principal Investigator トランスジェニックマウス / 薬剤副作用 / HLA / 肝障害 / PITT法 / トランスジェックマウス作製 / HLA発現確認 / トランスジェックマウス / CRISPR/Cas9 / 一本鎖DNA … More Except Principal Investigator ゲノム編集 / マウス / CRISPR / エレクトロポレーション / in vivo / GONAD / i-GONAD / CRISPR/Cas9 / 白内障 / piggyBac Less

in vivoゲノム編集効率の最適化と疾患モデル動物遺伝子治療への応用Principal InvestigatorOngoing
- Principal Investigator
  
  三浦浩美
- Project Period (FY)
  2019 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 42040:Laboratory animal science-related
- Research Institution
  Tokai University
Easi-CRISPR for creating genetically modified mice using long ssDNA.Principal Investigator
- Principal Investigator
  
  MIURA Hiromi
- Project Period (FY)
  2016 – 2017
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Integrative animal science
- Research Institution
  Tokai University
Development of novel method for generation of genome editing mice without ex vivo handling of embryos
- Principal Investigator
  
  OHTSUKA Masato
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Laboratory animal science
- Research Institution
  Tokai University
Developmentandapplicationofmouseandcellmodelsforanalyses of HLA-dependent adverse drug reactionsPrincipal Investigator
- Principal Investigator
  
  MIURA Hiromi
- Project Period (FY)
  2012 – 2014
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Laboratory animal science
- Research Institution
  Tokai University

All 2018 2017 2016 2015 2013 2012 Other

All Journal Article Presentation Book Patent

[Book] PITT法によるターゲットトランスジェニックマウス作製　（動物／疾患モデルの作製技術・病態解析・評価手法）2017
- Author(s)
  大塚正人、三浦浩美
- Total Pages
  428
- Publisher
  技術情報協会
- Data Source
  KAKENHI-PROJECT-16K18821
[Journal Article] Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors2018
- Author(s)
  Miura H, Quadros RM, Gurumurthy CB, Ohtsuka M
- Journal Title
  
  Nature Protocols
  
  Volume: 13 Pages: 195-215
- DOI
  10.1038/nprot.2017.153
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H04685, KAKENHI-PROJECT-16K18821
[Journal Article] i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases2018
- Author(s)
  Ohtsuka Masato、Sato Masahiro、Miura Hiromi、Takabayashi Shuji、Matsuyama Makoto、Koyano Takayuki、Arifin Naomi、Nakamura Shingo、Wada Kenta、Gurumurthy Channabasavaiah B.
- Journal Title
  
  Genome Biology
  
  Volume: 19 Pages: 1-15
- DOI
  10.1186/s13059-018-1400-x
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K15063, KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-15K14371, KAKENHI-PROJECT-16K18821, KAKENHI-PROJECT-16K07087
[Journal Article] Easi-CRISPR: A robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins.2017
- Author(s)
  Quadros RM, Miura H, Harms DW, Akatsuka H, Sato T, Aida T, Redder R, Richardson GP, Inagaki Y, Sakai D, Buckley SM, Seshacharyulu P, Batra SK, Behlke MA, Zeiner SA, Jacobi AM, Izu Y, Thoreson WB, Urness LD, Mansour SL, Ohtsuka M, Gurumurthy CB
- Journal Title
  
  Genome Biol
  
  Volume: 18 Pages: 92-92
- DOI
  10.1186/s13059-017-1220-4
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K07085, KAKENHI-PROJECT-16H04685, KAKENHI-PROJECT-16K18821, KAKENHI-PROJECT-17H04166
[Journal Article] GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of embryos2016
- Author(s)
  Gurumurthy C.B., Takahashi G., Wada K., Miura H., Sato M., and Ohtsuka M.
- Journal Title
  
  Curr. Protoc. Hum. Genet.
  
  Volume: 88
- DOI
  10.1002/0471142905.hg1508s88
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Journal Article] The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs2016
- Author(s)
  Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.
- Journal Title
  
  Int J Mol Sci
  
  Volume: 17 Pages: 1424-1424
- DOI
  10.3390/ijms17091424
- NAID
  130007023583
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-25293418, KAKENHI-PROJECT-25463192, KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-16K08085, KAKENHI-PROJECT-16K11810, KAKENHI-PROJECT-15K14371, KAKENHI-PROJECT-16K15063
[Journal Article] GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice2015
- Author(s)
  Takahashi G, Gurumurthy CB, Wada K, Miura H, Sato M, Ohtsuka M
- Journal Title
  
  Sci Rep
  
  Volume: 5 Pages: 11406-11406
- DOI
  10.1038/srep11406
- Peer Reviewed / Acknowledgement Compliant / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-15K14371
[Journal Article] One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).2015
- Author(s)
  Ohtsuka M., Miura H., Mochida K., Hirose M., Hasegawa A., Ogura A., Mizutani R., Kimura M., Isotani A., Ikawa M., Sato M. and Gurumurthy C. B.
- Journal Title
  
  BMC Genomics.
  
  Volume: 16 Pages: 274-274
- DOI
  10.1186/s12864-015-1432-5
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-24700435, KAKENHI-PROJECT-15K07695, KAKENHI-INTERNATIONAL-15K21737, KAKENHI-ORGANIZER-25112001, KAKENHI-PROJECT-25250014, KAKENHI-PROJECT-25290035
[Journal Article] PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells2015
- Author(s)
  Inada E, Saito I, Watanabe S, Aoki R, Miura H, Ohtsuka M, Murakami T, Sawami T, Yamasaki Y, Sato M
- Journal Title
  
  Int J Oral Sci.
  
  Volume: 7 Pages: 144-145
- DOI
  10.1038/ijos.2015.18
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-25463192, KAKENHI-PROJECT-15K14371, KAKENHI-PROJECT-15J03924, KAKENHI-PROJECT-25293418
[Journal Article] piggyBac-mediated generation of stable transfectants with surface HLA expression from a small number of cells.2013
- Author(s)
  Miura H., Inoko H., Inoue I., Okada Y., Tanaka M., Sato M. and Ohtsuka M.
- Journal Title
  
  Anal Biochem
  
  Volume: 437 Pages: 29-31
- DOI
  10.1016/j.ab.2013.02.003
- Peer Reviewed
- Data Source
  KAKENHI-PLANNED-22133002, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-24700435, KAKENHI-PROJECT-25290035
[Journal Article] Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination.2013
- Author(s)
  Ohtsuka M, Miura H, Hayashi H, Nakaoka H, Kimura M, Sato M, Gurumurthy CB, Inoko H
- Journal Title
  
  Transgenic Res.
  
  Volume: 22 Pages: 873-875
- DOI
  10.1007/s11248-013-9703-x
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24700435, KAKENHI-PROJECT-25290035, KAKENHI-PROJECT-24651064
[Journal Article] PITT: Pronuclear Injection-Based Targeted Transgenesis, a Reliable Transgene Expression Method in Mice2012
- Author(s)
  Ohtsuka M, Miura H, Sato M, Kimura M, Inoko H, Gurumurthy CB
- Journal Title
  
  Experimental Animals
  
  Volume: 61 Issue: 5 Pages: 489-502
- DOI
  10.1538/expanim.61.489
- NAID
  10031121853
- ISSN
  1341-1357, 1881-7122
- Language
  English
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24700435, KAKENHI-PLANNED-22133002
[Journal Article] Fluorescent transgenic mice suitable for multi-color aggregation chimera studies.2012
- Author(s)
  Ohtsuka M, Miura H, Gurumurthy CB, Kimura M, Inoko H, Yoshimura S, Sato M
- Journal Title
  
  Cell Tissue Res.
  
  Volume: 350 Pages: 251-260
- DOI
  10.1007/s00441-012-1470-0
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-24700435, KAKENHI-PROJECT-24651064, KAKENHI-PLANNED-22133002
[Journal Article] Determination of the optimal concentration of several selective drugs useful for generating multi-transgenic porcine embryonic fibroblasts.2012
- Author(s)
  Sato M, Ohtsuka M, Miura H, Miyoshi K, Watanabe S.
- Journal Title
  
  Reprod Domest Anim.
  
  Volume: 47 Pages: 759-765
- DOI
  10.1111/j.1439-0531.2011.01964.x
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350, KAKENHI-PROJECT-22580321, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-24700435, KAKENHI-PLANNED-22133002
[Patent] Dna editing using single-stranded dna2016
- Inventor(s)
  CB GURU, H Miura, M Ohtsuka
- Industrial Property Rights Holder
  Nebraska Univ., Tokai Univ.
- Industrial Property Rights Type
  特許
- Filing Date
  2016-06-03
- Overseas
- Data Source
  KAKENHI-PROJECT-16K18821
[Patent] 標的遺伝子のノックダウン方法およびそのためのRNAi用発現ベクター2012
- Inventor(s)
  大塚正人、三浦浩美
- Industrial Property Rights Holder
  大塚正人、三浦浩美
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2012-259950
- Filing Date
  2012-11-28
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] Development of a reporter mouse model suitable for evaluation of in vivo genome editing efficiency2018
- Author(s)
  H Miura, CB Gurumurthy, M Ohtsuka
- Organizer
  2018 Cold Spring Harbor meeting: Genome Engineering: The CRISPR/Cas Revolution
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K06040
[Presentation] ゲノム編集効率評価系モデルマウスの開発と評価2018
- Author(s)
  三浦浩美、佐藤正宏、水谷晃子、大塚正人
- Organizer
  第41回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-18K06040
[Presentation] 採卵、顕微注入、胚移植のステップが不要なゲノム編集マウス作製法GONADによるノックインマウス作製2017
- Author(s)
  大塚正人, 三浦浩美, 佐藤正宏
- Organizer
  日本ゲノム編集学会第2回大会
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Development of mouse model suitable for in vivo genome editing efficiency studies2017
- Author(s)
  Hiromi Miura, Yutaka Inagaki, Channabasavaiah B Gurumurthy, Masahiro Sato, Masato Ohtsuka
- Organizer
  14th Transgenic Thecnology Meeting
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K18821
[Presentation] CRISPR solution for creating Cre-LoxP genetics resources2017
- Author(s)
  Rolen M Quadros, Hiromi Miura, Masato Ohtsuka, Donald W Harms, Channabasavaiah B Gurumurthy
- Organizer
  Genome Engineering: The CRISPR-Cas Revolution(CSHL Meetings & Courses Program)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K18821
[Presentation] Rescue of the albino phenotype in mice using GONAD method2017
- Author(s)
  Hiromi Miura, Naomi Arifin, Masahiro Sato, Masato Ohtsuka
- Organizer
  International Pigment Cell Conference 2017(IPCC2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16K18821
[Presentation] Development of a mouse model suitable for in vivo genome editing efficiency studies2017
- Author(s)
  Hiromi Miura, Yutaka Inagaki,Channabasavaiah B Gurumurthy, Masahiro Sato, Masato Ohtsuka
- Organizer
  14th Transgenic Technology Meeting (TT2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] In vivoゲノム編集効率評価系モデルマウスの開発2017
- Author(s)
  三浦浩美、稲垣豊、佐藤正宏、大塚正人
- Organizer
  第40回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-16K18821
[Presentation] Rescue of the albino phenotype in mice using GONAD method2017
- Author(s)
  Hiromi Miura, Naomi Arifin, Masahiro Sato, Masato Ohtsuka
- Organizer
  International Pigment Cell Conference 2017(IPCC2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Improved GONAD (i-GONAD) (I) as ex vivo manipulation-free genome-editing system allowing efficient knock-out, large deletion, and knock-in2017
- Author(s)
  Masato Ohtsuka, Hiromi Miura, Channabasavaiah B. Gurumurthy, Masahiro Sato
- Organizer
  14th Transgenic Technology Meeting (TT2017)
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] In vivoゲノム編集効率評価系モデルマウスの開発2017
- Author(s)
  三浦浩美、稲垣豊、佐藤正宏、大塚正人
- Organizer
  第40回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] ex vivo胚操作が不要なゲノム編集マウス作製法2016
- Author(s)
  大塚正人, 高橋　剛, 和田　健太, 三浦浩美, 佐藤正宏
- Organizer
  平成28年度新学術領域研究「学術研究支援基盤形成【先端モデル動物支援プラットフォーム】」若手支援技術講習会
- Place of Presentation
  蓼科グランドホテル滝の湯（長野県茅野市）
- Year and Date
  2016-09-14
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 長い一本鎖DNAを用いたノックインマウス作製2016
- Author(s)
  三浦浩美、Channabasavaiah Gurumurthy、稲垣豊、大塚正人
- Organizer
  平成28年度新学術領域研究「学術研究支援基盤形成【先端モデル動物支援プラットフォーム】」若手支援技術講習会
- Place of Presentation
  蓼科グランドホテル滝の湯(長野県茅野市)
- Year and Date
  2016-09-14
- Data Source
  KAKENHI-PROJECT-16K18821
[Presentation] Easi-CRISPR：長鎖一本鎖DNAを用いた高効率ノックイン法2016
- Author(s)
  大塚正人，相田知海，三浦浩美，Channabasavaiah Gurumurthy
- Organizer
  日本ゲノム編集学会第1回大会
- Place of Presentation
  広島国際会議場(広島県広島市)
- Year and Date
  2016-09-06
- Data Source
  KAKENHI-PROJECT-16K18821
[Presentation] 採卵、顕微注入、胚移植を要しないゲノム編集マウス作製法GONADによるノックアウトマウス作製2016
- Author(s)
  大塚正人, 高橋　剛, 和田　健太, 三浦浩美, Channabasavaiah B Gurumurthy, 佐藤正宏
- Organizer
  第63回日本実験動物学会総会
- Place of Presentation
  ミューザ川崎シンフォニーホール（神奈川県川崎市）
- Year and Date
  2016-05-18
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] GONAD法：採卵、顕微注入、胚移植のステップが不要なゲノム編集マウス作製法2016
- Author(s)
  大塚正人、高橋剛、三浦浩美、和田健太、佐藤正宏
- Organizer
  第39回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜（神奈川県横浜市）
- Year and Date
  2016-11-30
- Invited
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Easi-CRISPR：高効率なノックインマウス作製法2016
- Author(s)
  三浦浩美、Gurumurthy Channabasavaiah、相田知海、稲垣豊、佐藤正宏、佐藤健人、大塚正人
- Organizer
  第39回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜(神奈川県横浜市)
- Year and Date
  2016-11-30
- Data Source
  KAKENHI-PROJECT-16K18821
[Presentation] GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of fertilized eggs2015
- Author(s)
  Masato Ohtsuka, Gou Takahashi, Kenta Wada, Hiromi Miura, Channabasavaiah B Gurumurthy, Masahiro Sato
- Organizer
  29th International Mammalian Genome Conference (IMGC)
- Place of Presentation
  Yokohama Port Opening Memorial Hall, (Yokohama, Kanagawa, Japan)
- Year and Date
  2015-11-08
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 卵管を介したマウス 2 細胞期胚への RNA 導入法の開発とゲノム編集技術への応用2015
- Author(s)
  高橋剛、和田健太、三浦浩美、佐藤正宏、大塚正人
- Organizer
  第62回日本実験動物学会総会
- Place of Presentation
  京都テルサ（京都府京都市）
- Year and Date
  2015-05-28
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] GONAD：採卵、顕微注入、移植を要しない新規ゲノム編集マウス作製法2015
- Author(s)
  大塚正人、Channabasavaiah B Gurumurthy、三浦浩美、佐藤正宏、和田健太、高橋剛
- Organizer
  第108回日本繁殖生物学会大会
- Place of Presentation
  宮崎大学木花キャンパス　（宮崎県宮崎市）
- Year and Date
  2015-09-17
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] ex vivo 処置を介さない CRISPR/Cas9 系による遺伝子改変マウス作製法「GONAD」の開発2015
- Author(s)
  高橋剛、Channabasavaiah B Gurumurthy、和田健太、三浦浩美、佐藤正宏、大塚正人
- Organizer
  第 12 回北海道実験動物研究会総会・学術集会
- Place of Presentation
  北海道大学大学院医学研究科臨床講義棟第三講堂　（北海道札幌市）
- Year and Date
  2015-07-11
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] 採卵、顕微注入、胚移植を要しない新規ゲノム編集マウス作製法2015
- Author(s)
  高橋剛、和田健太、三浦浩美、佐藤正宏、大塚正人
- Organizer
  第 29 回モロシヌス研究会
- Place of Presentation
  神戸かんぽの宿有馬　（兵庫県神戸市）
- Year and Date
  2015-07-03
- Data Source
  KAKENHI-PROJECT-15K14371
[Presentation] Neuropathy target esterase（神経障害標的エステラーゼ）遺伝子導入マウスの作成2013
- Author(s)
  加賀谷徹、割田貴之、三浦浩美、大塚正人、大久保朋一、坂部貢、木村穣
- Organizer
  第22回日本臨床環境医学会学術集会
- Place of Presentation
  北里大学白金キャンパス
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] Generation of Knockdown Mice Using Pronuclear Injection-Based Targeted Transgenesis: Advantages and Limitations2013
- Author(s)
  Hiromi Miura, Masato Ohtsuka
- Organizer
  The 27th International Mammalian Genome Conference
- Place of Presentation
  Salamanca, Spain
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] 受精卵へのiCre mRNA注入によるターゲットトランスジェネシス法（PITT法）の効率改善2013
- Author(s)
  大塚正人、三浦浩美、佐藤正宏、木村穣
- Organizer
  第60回日本実験動物学会総会
- Place of Presentation
  つくば国際会議場
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] トランスポゾンPiggyBacシステムによる複数遺伝子のブタ細胞への同時導入2013
- Author(s)
  郡山美優、稲田絵美、齋藤一誠、三浦浩美、大塚正人、中村伸吾、桜井敬之、渡部聡、三好和睦、佐藤正宏
- Organizer
  第106回日本繁殖生物学会
- Place of Presentation
  東京農工大学農学部府中キャンパス
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] PITT法を利用して作製したノックダウンマウスにおけるノックダウン効果の比較解析2012
- Author(s)
  三浦浩美、佐藤正宏、大塚正人
- Organizer
  第35回日本分子生物学会年会
- Place of Presentation
  福岡国際会議場・マリンメッセ福岡
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] Recombinase及びIntegraseによる部位特異的遺伝子導入効率の比較検討2012
- Author(s)
  大塚正人、三浦浩美、木村穣、猪子英俊
- Organizer
  第59回日本実験動物学会総会
- Place of Presentation
  別府国際コンベンションセンター
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] iCre mRNA注入による次世代型トランスジェニックマウス作製法（PITT）の効率改善2012
- Author(s)
  大塚正人、佐藤正宏、三浦浩美
- Organizer
  第35回日本分子生物学会年会
- Place of Presentation
  福岡国際会議場・マリンメッセ福岡
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] Concurrent production of multiple targeted transgenic mouse lines with C57BL/6N genetic background by improved PITT
- Author(s)
  Masato Ohtsuka, Hiromi Miura, Minoru Kimura, Ayako Isotani, Masahito Ikawa, Masahiro Sato, Channabasavaiah Gurumurthy
- Organizer
  12th Transgenic Technology Meeting (TT2014)
- Place of Presentation
  Edinburgh, Scotland, United Kingdom
- Year and Date
  2014-10-06 – 2014-10-08
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] 薬剤副作用の発症機序解明の為の、HLA遺伝子発現細胞とHLAトランスジェニックマウスの作製
- Author(s)
  三浦浩美、大塚正人
- Organizer
  第37回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2014-11-25 – 2014-11-27
- Data Source
  KAKENHI-PROJECT-24700435
[Presentation] C57BL/6Nを遺伝的背景に持つターゲットトランスジェネシス用種マウスの作製と評価
- Author(s)
  大塚正人, 三浦浩美, 磯谷綾子, 伊川正人, 佐藤正宏, 木村穣
- Organizer
  第61回日本実験動物学会総会
- Place of Presentation
  札幌コンベンションセンター
- Year and Date
  2014-05-15 – 2014-05-17
- Data Source
  KAKENHI-PROJECT-24700435

1. OHTSUKA Masato (90372945)

# of Collaborated Projects: 1 results

# of Collaborated Products: 26 results
2. SATO Masahiro (30287099)

# of Collaborated Projects: 1 results

# of Collaborated Products: 24 results
3. 和田健太 (20508113)

# of Collaborated Projects: 1 results

# of Collaborated Products: 11 results
4. INOKO Hidetoshi

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
5. ISOTANI Ayako

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
6. AIDA Tomomi

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
7. INAGAKI Yutaka

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
8. INADA Emi

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
9. NAKAMURA Shingo

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
10. 伊川正人

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
11. 小倉淳郎

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
12. 持田慶司

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
13. 齊藤一誠

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
14. 山崎要一

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
15. 齊藤陽子

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
16. 三好和睦

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
17. 木村穣

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
18. 高林秀次

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Miura Hiromi 三浦 浩美

三浦 浩美 ミウラ ヒロミ

MIURA Hiromi 三浦 浩美

Research Projects

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in vivoゲノム編集効率の最適化と疾患モデル動物遺伝子治療への応用Principal InvestigatorOngoing

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Easi-CRISPR for creating genetically modified mice using long ssDNA.Principal Investigator

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Development of novel method for generation of genome editing mice without ex vivo handling of embryos

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Developmentandapplicationofmouseandcellmodelsforanalyses of HLA-dependent adverse drug reactionsPrincipal Investigator

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[Book] PITT法によるターゲットトランスジェニックマウス作製 （動物／疾患モデルの作製技術・病態解析・評価手法）2017

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[Journal Article] Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors2018

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[Journal Article] i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases2018

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[Journal Article] Easi-CRISPR: A robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins.2017

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[Journal Article] GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of embryos2016

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[Journal Article] The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs2016

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[Journal Article] GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice2015

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[Journal Article] One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).2015

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[Journal Article] PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells2015

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[Journal Article] piggyBac-mediated generation of stable transfectants with surface HLA expression from a small number of cells.2013

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Miura Hiromi 三浦浩美

三浦浩美ミウラヒロミ

MIURA Hiromi 三浦浩美

[Book] PITT法によるターゲットトランスジェニックマウス作製　（動物／疾患モデルの作製技術・病態解析・評価手法）2017