Novel cell surface genes expressed in the stomach primordium during gastrointestinal morphogenesis of mouse embryos
Highlights
► 3 Novel cell surface genes expressed in the stomach primordium were identified. ► Adra2a is expressed in the mesenchyme of the mouse stomach primordium at E11.5. ► Fzd5 and Trpv6 are expressed in the epithelial layer of the stomach primordium after E11.5.
Section snippets
Results and discussion
During mouse gastrulation, development of the embryonic endoderm is initiated by an evolutionally conserved gene regulatory network. Nodal, a member of the transforming growth factor-β superfamily, is one of the major growth factors that promote endoderm development. High levels of nodal expression, maintained by a positive autoregulatory loop, induce the conserved network of transcription factors for endoderm development (Shen, 2007). The resulting endodermal cells then morphologically
Embryo collection
Mouse embryos were collected from pregnant ICR mice (E9.5–E17.5) in ice-cold PBS. The embryos were fixed in 4% paraformaldehyde in PBS solution overnight at 4 °C with gentle shaking. The embryos were washed in PBS for 10 min at 4 °C and dehydrated using a series of methanol-PBS washes. The embryos were then stored in 100% methanol at −20 °C until in situ hybridization analysis. Newborn mice were also fixed after being anesthetized. All animal experiments were conducted according to the committee
Acknowledgments
This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (M.A. and A.K.) and a research grant from the Suzuken Memorial Foundation (A.K.).
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2018, Gene Expression PatternsCitation Excerpt :Trpv5, on the contrary, only has reported expression data at E11.5 and E15.5 in mouse (Magdaleno et al., 2006). Data on trpv6 embryonic expression pattern is available only after E9.5 and at 3dpf in zebrafish (Magdaleno et al., 2006; Diez-Roux et al., 2011; Naguchi et al., 2012). Given the importance and versatility of trpv channels, a more comprehensive characterization during development will provide important insight into some of their earlier functions.
Generation of stomach tissue from mouse embryonic stem cells
2015, Nature Cell Biology