Studies for preventive biomarkers based on genotypes and gene expression regulation system
Project/Area Number |
16K15380
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Hygiene and public health
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Research Institution | Wakayama Medical University |
Principal Investigator |
Mure Kanae 和歌山県立医科大学, 医学部, 准教授 (90268491)
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Co-Investigator(Kenkyū-buntansha) |
竹下 達也 和歌山県立医科大学, 医学部, 教授 (20150310)
島袋 美絵 和歌山県立医科大学, 医学部, 学内助教 (70776939)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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Keywords | 動脈硬化 / 遺伝子型 / miRNA / エクソソーム / マイクロRNA / バイオマーカー / 遺伝子発現 / ゲノム編集 |
Outline of Final Research Achievements |
In the present study, the genotypes and exosomal miRNA associated with the endothelial dysfunction and oxidative stresses have analyzed as preventive markers that can predict the onset of diseases related to aging. As for the genotypes, PPARG 125 C/G polymorphism (rs1801282), the PON1 672 A/G polymorphism (rs662) and PLA2G7 994 A/C polymorphism (rs76863441) have shown significant relationships between arteriosclerosis indices. Soluble oxidized LDL receptor and urinary 8-iso-prostaglandin F2α were also associated with those indices. Furthermore, exosomal miR-126 and miR-21 showed significant association with arteriosclerosis indices. Taken together these results, it is suggested that the genotype analyzed in this study and miRNA in blood and urine exosomes could be preventive markers.
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Academic Significance and Societal Importance of the Research Achievements |
本研究では、疾患診断治療領域において精力的に機構解明研究が行われているmRNAを標的とした遺伝子発現制御機構であるマイクロRNA(miRNA)について、一般住民を対象とした大規模健診において、加齢に伴う疾患の共通発症基盤と考えられる血管内皮機能障害や酸化ストレスの鋭敏な指標となるかについて、遺伝子型の影響も含めて検討した。 本研究より、血中および尿中エクソソーム内包miRNAが血中コレステロールや酸化ストレス指標と関連することが明らかとなった。解析対象者を増やし、遺伝子型との関連についても総合的に解析することで、鋭敏なバイオマーカーになり得るかについて検討する基盤となる成果が得られたと考える。
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Relationships of alcohol dehydrogenase 1B (ADH1B) and aldehyde dehydrogenase 2 (ALDH2) genotypes with alcohol sensitivity, drinking behavior and problem drinking in Japanese older men2016
Author(s)
Hashimoto M, Watanabe M, Uematsu Y, Hattori S, Miyai N, Utsumi M, Oka M, Hayashida M, Kinoshita K, Arita M, Takeshita T
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Journal Title
Environ Health Prev Med
Volume: 21
Issue: 3
Pages: 138-148
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] 酸化LDL受容体遺伝子OLR1多型が血圧・糖代謝・血中脂質・炎症指標に与える影響は服薬状況により異なる2019
Author(s)
海老泰文,牟礼佳苗,中喬弘,上杉弥優,服部園美,宮井信行,内海みよ子,上松右二,志波充,有田幹雄,竹下達也
Organizer
第89回日本衛生学会学術総会
Related Report
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[Presentation] 抗酸化酸素PON1遺伝子型が血圧・糖代謝・血中脂質・炎症指標に与える影響は服薬状況により異なる2019
Author(s)
中喬弘,牟礼佳苗,海老泰文,上杉弥優,服部園美,宮井信行,内海みよ子,上松右二,志波充,有田幹雄,竹下達也
Organizer
第89回日本衛生学会学術総会
Related Report
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[Presentation] The impact of smoking on central aortic pressure response to exercise in middle-aged normotensive individuals2017
Author(s)
Miyai N, Shiozaki M, Nagatomo N, Uchikawa Y, Terada K, Tomida K, Mure K, Utsumi M, Takeshita T, Miyashita K, Arita M
Organizer
27th European Meeting on Hypertension and Cardiovascular Protection
Related Report
Int'l Joint Research
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