Novel methods to regulate hepatic graft by extracorporial perfusion : Chaperoen mediated mechanisms
Project/Area Number |
26670572
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General surgery
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Research Institution | Hokkaido University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
深井 原 北海道大学, 医学研究科, 特任助教 (60374344)
木村 太一 北海道大学, 医学研究科, 特任助教 (90435959)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 移植・再生医療 / 外科 / 発現制御 / 臓器灌流 / 虚血再灌流 / 臓器保存 / 抗酸化 / 肝移植 / 脂肪肝 / シャペロン |
Outline of Final Research Achievements |
The role of 14-3-3 zeta on the cellular injury and energy production during and after hypothermic storage with or without oxygen, using mouse hepatocytes derived cell line (AML12). 14-3-3 zeta overexpression was introduced by gene delivery or some small molecule. After confirmation of 14-3-3 zeta protein level, cells were subjected to hypothermic oxygenate condition mimicking the cellular environment during organ perfusion. Combination of 14-3-3 zeta inducing molecule and hydrogen sulfide strengthen the mitochondrial functions related to oxidative phosphorylation, drug (MTT) metabolism, and antioxidant ability, due to the prevention of protein dephosphorylation (14-3-3) and stimulation of ARE-binding transcription factors (NaHS). These results revealed novel strategy to pre-condition the marginal graft not by gene delivery but by transcriptional and post-translational regulations.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Hydrogen Gas Ameliorates Hepatic Reperfusion Injury After Prolonged Cold Preservation in Isolated Perfused Rat Liver2016
Author(s)
Shimada S, Wakayama K, Fukai M, Shimamura T, Ishikawa T, Fukumori D, Shibata M, Yamashita K, Kimura T, Todo S, Ohsawa I, Taketomi A
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Journal Title
Artif Organs
Volume: 40
Issue: 12
Pages: 1128-1136
DOI
Related Report
Peer Reviewed
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[Journal Article] 14-3-3ζ-mediated stimulation of oxidative phosphorylation exacerbates oxidative damage under hypothermic oxygenated conditions in human renal tubular cells (HK-2)2016
Author(s)
Fukai M, Kobayashi N, Ishikawa T, Wakayama K, Shimada S, Umemoto K, Ohtani S, MFujiyoshi M, Yamashita K, Shimamura T, Taketomi A
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Journal Title
Transplant Proc.
Volume: 48
Issue: 4
Pages: 1288-1291
DOI
Related Report
Peer Reviewed
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[Journal Article] A pilot study of operational tolerance with a regulatory T cell-based cell therapy in living donor liver transplantation.2016
Author(s)
①Todo S, Yamashita K, Goto R, Zaitsu M, Nagatsu A, Oura T, Watanabe M, Aoyagi T, Suzuki T, Shimamura T, Kamiyama T, Sato N, Sugita J, Hatanaka K, Bashuda H, Habu S, Demetris AJ, Okumura K
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Journal Title
Hepatology
Volume: 00
Issue: 2
Pages: 00-00
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] 14-3-3ζ-mediated stimulation of oxidative phosphorylation exacerbates oxidative damage under hypothermic oxygenated conditions in human renal tubular cells (HK-2)2016
Author(s)
Moto Fukai, Nozomi Kobayashi, Takahisa Ishikawa, Kenji Wakayama, Shingo Shimada, Kohei Umemoto, Shintaro Ohtani, Masato Fujiyoshi, Kenichiro Yamashita, Tsuyoshi Shimamura, and Akinobu Taketomi
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Journal Title
Transplantation Proceedings
Volume: in press
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Hydrogen sulfide augments survival signals in warm ischemia and reperfusion of the mouse liver.2015
Author(s)
Shimada S, Fukai M, Wakayama K, Ishikawa T, Kobayashi N, Kimura T, Yamashita K, Kamiyama T, Shimamura T, Taketomi A, Todo S
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Journal Title
Surg Today
Volume: 45
Pages: 892-903
Related Report
Peer Reviewed
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[Journal Article] Hydrogen sulfide augments survival signals in warm ischemia and reperfusion of the mouse liver.2015
Author(s)
Shimada, S., Fukai, M., Wakayama, K., Ishikawa, T., Kobayashi, N., Kimura, T., Yamashita, K., Kamiyama, T., Shimamura, T., Taketomi, A., Todo, S.
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Journal Title
Surgery Today
Volume: 45
Issue: 7
Pages: 892-903
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Establishment of educational program for multiorgan procurement from deseased donors.2014
Author(s)
Taniguchi M, Furukawa H, Kawai T, Morikawa H, Morozumi K, Goto M, Kondo T, Aikawa A, Ito T, Takahara S, Nio M, Kokudo N, Uemoto S, Fukushima N, Yoshida K, Kenmochi T, Date H, Ono M, Eguchi S, Shimamura T, Mizuta K, Yoshizumi T, Ueno T
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Journal Title
Transplant Proc
Volume: 46
Pages: 1071-1073
Related Report
Peer Reviewed
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[Presentation] Successful Withdrawal of Immunosuppression by a Cell Therapy Using Donor Ag-pulsed Tregs in Living Donor Liver Transplantation: An Update on Clinical Trial.2014
Author(s)
Kenichiro Yamashita, Masaki Zaitsu, Akihisa Nagatsu, Ryoichi Goto, Tetsu Oura, Masaaki Watanabe, Takeshi Aoyagi, Tomomi Suzuki, Tsuyoshi Shimamura, Toshiya Kamiyama, Norihiro Sato, Junichi Sugita, Kanako Hatanaka, Hisashi Bashuda, Ko Okumura, Satoru Todo
Organizer
The 2014 Joint International Congress of ILTS, ELITA & LICAGE
Place of Presentation
Queen Elizabeth II Conference Centre (London, UK)
Year and Date
2014-06-04 – 2014-06-07
Related Report
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[Presentation] Preoperative Prediction of Short-Term Prognosis after Adult-to-Adult Living Donor Liver Transplantation: A Way to Zero-Mortality.2014
Author(s)
Tsuyoshi Shimamura, Masato Fujiyoshi, Tomomi Suzuki, Kenichiro Yamashita, Takeshi Aoyagi, Ryoichi Goto, Toru Takahashi, Yasuyuki Koshizuka, Masahiko Taniguchi, Hiroyuki Furukawa, Satoru Todo
Organizer
The 2014 Joint International Congress of ILTS, ELITA & LICAGE
Place of Presentation
Queen Elizabeth II Conference Centre (London, UK)
Year and Date
2014-06-04 – 2014-06-07
Related Report
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