Abstract
Purpose
We recently reported frequent detection of antibiotic-resistant bacteria on the oral mucosa during the period of hematopoietic cell transplantation (HCT) and suggested an association between oral mucositis and antibiotic-resistant bacterial infection. Methicillin-resistant Staphylococcus spp. were frequently detected, and the oral cavity may be a reservoir of the gene mediating methicillin resistance, mecA. Here, we examined the frequency of mecA carriers in patients undergoing HCT.
Methods
Fifty-nine patients (male (M) = 37, female (F) = 22, 47.3 ± 11.0 years) receiving HCT were enrolled in this study. Buccal swab samples were obtained four times from day −7 to day +20 (once/week), and mecA was detected by PCR. Fifty-two subjects without systemic disease, who completed dental treatment, especially periodontal treatment (M = 21, F = 31, 55.4 ± 14.2 years), were also enrolled as controls and checked for mecA on the oral mucosa.
Results
Seventy-six percent (45/59) of the HCT patients carried mecA at least once in the study period (days −7 to +20), while no control subjects had mecA. The frequency of mecA carriers was 19.2 % from days −7 to −1, while it was significantly increased on days +7 to +13 and +14 to +20, with frequencies of 60.9 and 63.2 %, respectively (P < 0.01, ANOVA).
Conclusions
mecA was detected in oral mucosa of patients undergoing HCT. The high detection frequency of staphylococci resistant to penicillin and beta-lactams in our recent report was supported.
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Acknowledgments
This study was supported by a Grant-in-Aid for Young Scientists (B no. 22791836 and no. 24792024 to YS) from the Japan Society for the Promotion of Science, and a Health and Labour Sciences Research Grant (24120701) from the Ministry of Health, Labour and Welfare, Japan
Conflict of interest
We have no conflicts of interest in this study.
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This study was partly presented as a poster at the MASCC/ISOO 2012 International Symposium on Supportive Care in Cancer in New York City on June 28–30, 2012.
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Ebinuma, T., Soga, Y., Sato, T. et al. Distribution of oral mucosal bacteria with mecA in patients undergoing hematopoietic cell transplantation. Support Care Cancer 22, 1679–1683 (2014). https://doi.org/10.1007/s00520-014-2151-1
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DOI: https://doi.org/10.1007/s00520-014-2151-1