Issue 3, 2016

The application of fluorescence-conjugated pyrrole/imidazole polyamides in the characterization of protein–DNA complex formation

Abstract

N-Methylpyrrole (Py)–N-methylimidazole-(Im) polyamides are sequence-specific DNA-binding modules that are widely used for gene regulation, as synthetic transcriptional factors and as sequence-specific DNA alkylating agents. Recently, Py–Im polyamides have been conjugated with fluorophores, resulting in conjugates that are useful for the detection of specific DNA sequences. A Förster resonance energy transfer has been observed between Cy3- and Cy5-conjugated Py–Im polyamides on the nucleosome, indicating that fluorescence-conjugated Py–Im polyamides could possibly be used to characterise protein–DNA complexes. In this minireview, we discuss recent reports regarding fluorescence-conjugated Py–Im polyamides and their future application in the characterization of protein–DNA complex formation.

Graphical abstract: The application of fluorescence-conjugated pyrrole/imidazole polyamides in the characterization of protein–DNA complex formation

Article information

Article type
Minireview
Submitted
30 Jun 2015
Accepted
10 Dec 2015
First published
06 Jan 2016

Biomater. Sci., 2016,4, 391-399

The application of fluorescence-conjugated pyrrole/imidazole polyamides in the characterization of protein–DNA complex formation

Y. Han, H. Sugiyama and Y. Harada, Biomater. Sci., 2016, 4, 391 DOI: 10.1039/C5BM00214A

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