Structural basis for small RNA functional mechanism in eukaryotes
Project/Area Number |
25840019
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | Niigata University |
Principal Investigator |
Miyoshi Tomohiro 新潟大学, 研究推進機構超域学術院, 助教 (60534550)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | Argonaute / small RNA / 小分子RNA / 遺伝子発現制御 / X線結晶構造解析 / RNAサイレンシング / 小分子核酸 / non-coding RNA |
Outline of Final Research Achievements |
Argonaute proteins are widespread in all domains of life from bacteria to eukaryotes, and are an essential component of the nucleic acid-based silencing systems. Although many aspects of the structure of Argonaute-nucleic acid complexes have been described in previous crystal structure studies, the recognition mechanism of the guide and target strands by Argonaute proteins is still not fully understood. In the present study, we have solved the structure of Argonaute in complex with guide RNA and target DNA by X-ray crystallography.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Functional role of the C-terminal tail of the archaeal ribosomal stalk in recruitment of two elongation factors to the sarcin/ricin loop of 23S rRNA.2015
Author(s)
Imai, H, Miyoshi, T, Murakami, R, Ito, K, Ishino, Y, and Uchiumi, T.
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Journal Title
Genes. Cells
Volume: 20
Issue: 7
Pages: 613-624
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Solution structure of human P1-P2 heterodimer provides insights into the role of eukaryotic stalk in recruiting the ribosome-inactivating protein trichosanthin to the ribosome2013
Author(s)
Lee, K.M., Yusa, K., Chu, L.O., Yu, C.W., Oono, M., Miyoshi, T., Ito, K., Shaw, P.C., Wong, K.B., and Uchiumi, T
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Journal Title
Nucleic Acids Res
Volume: 41
Issue: 18
Pages: 8776-8787
DOI
Related Report
Peer Reviewed
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