Issue 18, 2013

Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

Abstract

A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

Graphical abstract: Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

Supplementary files

Article information

Article type
Communication
Submitted
30 Nov 2012
Accepted
11 Jan 2013
First published
15 Jan 2013

Chem. Commun., 2013,49, 1811-1813

Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

S. Morimoto, T. Tomohiro, N. Maruyama and Y. Hatanaka, Chem. Commun., 2013, 49, 1811 DOI: 10.1039/C3CC38594A

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