Control of lymphocyte chemotaxis by mitochondrial metabolism
Project/Area Number |
26670101
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
General physiology
|
Research Institution | University of Fukui |
Principal Investigator |
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | ミトコンドリア / ケモタクシス / 代謝 / システム生物学 |
Outline of Final Research Achievements |
Inhibition of mitochondrial Na+-Ca2+ exchanger (NCLX), which is strongly related with mitochondrial energy metabolism, caused attenuation of chemotaxis toward CXCL12 and augmentation of random cell movement in B lymphocytes (A20). The following mechanisms were elucidated; NCLX modulates actin polymerization and Rac1 localization through controlling cytoplasmic Ca2+ so that chemotaxis and cell motility of B lymphocytes are influenced. The control by NCLX was observed also in native B lymphocytes derived from mouse spleen, but not in T lymphocytes. It was demonstrated that the difference in Ca2+ dynamics in mitochondria and endoplasmic reticulum causes the preferential contribution of NCLX to chemotaxis in B lymphocytes. In addition, it was suggested that mitochondrial beta-oxidation has important roles in cell motility and chemotaxis of B lymphocytes.
|
Report
(3 results)
Research Products
(10 results)
-
-
[Journal Article] Tracking and quantification of dendritic cell migration and antigen trafficking between the skin and lymph nodes.2014
Author(s)
Tomura M, Hata A, Matsuoka S, Shand FH, Nakanishi Y, Ikebuchi R, Ueha S, Tsutsui H, Inaba K, Matsushima K, Miyawaki A, Kabashima K, Watanabe T, Kanagawa O.
-
Journal Title
Sci Rep.
Volume: 4
Issue: 1
Pages: 6030-6030
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
[Presentation] ミトコンドリアモデル2015
Author(s)
松岡 達、竹内綾子
Organizer
e-Heartシンポジウム
Place of Presentation
立命館大学びわこ・くさつキャンパス
Year and Date
2015-02-28 – 2015-03-01
Related Report
Invited
-
-
-
-
-