A heterogeneous tag-attachment to the homodimeric type 1 photosynthetic reaction center core protein in the green sulfur bacterium Chlorobaculum tepidum
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Research Highlights
► Homodimeric type 1 RC in green sulfur bacteria consists of two identical PscA core subunits. ► Insertion of the His-pscA gene into the recA region. ► The His-tagged RC complex has no PscD subunit but shows intrinsic electron transfer activity. ► Evidence of the heterogeneous tag-attachment by LC/MS/MS analysis. ► A site-directed mutagenesis on the His-pscA gene would be possible.
Abbreviations
RC
reaction center
PS
photosystem
cyt
cytochrome
ET
electron transfer
(B)Chl
(bacterio)chlorophyll
FMO
Fenna–Matthews–Olson
β-OG
n-octyl-β-d-glucopyranoside
ESP
Electron spin-polarization
Keywords
Green sulfur bacteria
Type 1 reaction center
Histidine tag
Electron paramagnetic resonance
LC/MS/MS
Iron–sulfur cluster
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