High-Throughput Genetic Identification of Regulatory Factors Involved in the Regulation of Seed Oil Storage Using Bioluminescence Automatic Monitoring System
Project/Area Number |
23380057
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied biochemistry
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Research Institution | Chubu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
ISHIURA Masahiro 名古屋大学, 遺伝子実験施設, 教授 (20132730)
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2011: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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Keywords | 種子成熟 / 種子発芽 / 油脂貯蔵 / 突然変異株 / 発光レポーター / 次世代シーケンサー / 植物種子 / 油脂 / 遺伝子発現抑制 / ルシフェラーゼ発光 / オレオシン / 突然変異体 / 次世代シーケンサ |
Outline of Final Research Achievements |
We prepared Arabidopsis plants that express LUC reporter gene under the control of promoters from genes important in the biosynthesis and storage of seed oil. By using automatic bioluminescence monitoring system, we screened several hundred thousands of plants for mutants with altered expression patterns of LUC reporter gene. In addition to AOTA1, a novel factor involved in the activation of DGAT1 gene for the final step of seed oil synthesis, we could identify several additional candidate genes potentially involved in the activation of DGAT1. Furthermore, we could identify a novel gene DROL1 coding for a factor potentially involved in the repression of OleS3 gene for seed oil storage after seed germination.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] A duplicated NUCLEOLIN gene in Arabidopsis (AtNUC2) is required for rDNA methylation and organization of silent 45S rRNA genes.2014
Author(s)
Durut, N., Abou-Ellail, M., Pontvianne, F., Comella, P., Chandra Das., S, Kojima, H., Ukai, S., DeBures, A., Echeverria, M., Bouvet, P., Nakamura, K. and Saez-Vasquez, J.
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Journal Title
Plant Cell
Volume: 26
Issue: 3
Pages: 1330-1344
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The roles of the dimeric and tetrameric structures of the clock protein KaiB in the generation of circadian oscillations in cyanobacteria2012
Author(s)
Murakami R, Mutoh R, Iwase R, Furukawa Y, Imada K, Onai K, Morishita M, Yasui S, Ishii K, Valencia Swain JO, Uzumaki T, Namba K, Ishiura M.
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Journal Title
J Biol Chem.
Volume: 287
Issue: 35
Pages: 29506-29515
DOI
Related Report
Peer Reviewed
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