JapanesePlex: a method for identification of being Japanese using Japanese-specific SNPs
Project/Area Number |
23590849
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Legal medicine
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Research Institution | Tottori University |
Principal Investigator |
YUASA Isao 鳥取大学, 医学部, 准教授 (00093633)
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Co-Investigator(Renkei-kenkyūsha) |
UMETSU Kazuo 山形大学, 医学部, 准教授 (10091828)
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Research Collaborator |
AKANE Atsushi 関西医科大学, 医学部, 教授 (70202520)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 日本人 / 特異遺伝子 / 個人識別 / PCR / DNA解析 / 集団特異遺伝子 / PCR / DNA解析 |
Research Abstract |
To find out Japanese-specific SNPs, a total of 875 DNA samples from Japanese in Tottori and Okinawa, Koreans, Mongolians, Hans, Tibetans, Germans and Africans were investigated. Sixty-seven (nearly) Japanese-specific SNPs were observed. Of them, 57 showed the highest frequencies in Okinawa, and 43 were also observed in Koreans at lower frequencies. DNA samples from Jomon remains in Hokkaido were investigated for three SNPs at the GALNT11, H19 and PLA2G12A genes, and showed higher frequencies in every SNP than those from Okinawa, suggesting that the SNPs observed at the highest frequencies in Okinawa were of Jomon lineage. For forensic practice, 50 SNPs with high frequency and no linkage disequilibrium were selected. A multiplex typing method based on single-based primer extension technology were established. This JapanesePlex method permitted the identification of being Japanese with high probability.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] De novo mutations in SLC35A2 encoding a UDP-galactose transporter cause early-onset epileptic encephalopathy2013
Author(s)
Kodera H, Nakamura K, Osaka H, Maegaki Y, Haginoya K, Mizumoto S, Kato M, Okamoto N, Iai M, Kondo Y, Nishiyama K, Tsurusaki Y, Nakashima M, Miyake N, Hayasaka K, Sugahara K, Yuasa I, Wada Y, Matsumoto N, Saitsu H
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Journal Title
Human Mutation
Volume: 34(12)
Pages: 1708-1714
Related Report
Peer Reviewed
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[Journal Article] De novo mutations in SLC35A2 encoding a UDP-galactose transporter cause early-onset epileptic encephalopathy2013
Author(s)
Kodera H, Nakamura K, Osaka H, Maegaki Y, Haginoya K, Mizumoto S, Kato M, Okamoto N, Iai M, Kondo Y, Nishiyama K, Tsurusaki Y, Nakashima M, Miyake N, Hayasaka K, Sugahara K, Yuasa I, Wada Y, Matsumoto N, Saitsu H.
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Journal Title
Hum Mutat
Volume: 34
Issue: 12
Pages: 1708-1714
DOI
Related Report
Peer Reviewed
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[Journal Article] Non-synonymous single-nucleotide polymorphisms of the human apoptosis- related endonuclease–DNA fragmenta- tion factor beta polypeptide, endo- nuclease G, and flap endonuclease-1–genes show a low degree of genetic heterogeneity2012
Author(s)
Takeshita H, Fujihara J, Ueki M, Iida R, Koda Y, Soejima M, Yuasa I, Kato H, Nakajima T, Kominato Y, Yasuda T
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Journal Title
DNA and Cell Biology
Volume: 31(1)
Pages: 36-42
Related Report
Peer Reviewed
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[Journal Article] Three Japanese patients with beta- ketothiolase deficiency who share a mutation, c.431A>C (H144P) in ACAT1: subtle abnormality in urinary organic acid analysis and blood acylcarnitine analysis using tandem mass spectrome- try2012
Author(s)
Fukao T, Maruyama S, Ohura T, Hasegawa Y, Toyoshima M,. Haapalainen AM, Kuwada N, Imamura M, Yuasa I, Wierenga RK, Yamaguchi S, Kondo N
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Journal Title
JIMD Reports
Volume: 3
Pages: 107-115
Related Report
Peer Reviewed
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[Journal Article] Transferrin mutations at the glycosylation site complicate diagnosis of congenital disorders of glycosylation type I2011
Author(s)
Guillard M, Wada Y, Hansikova H, Yuasa I, Vesela K, Ondruskova N, Kadoya M, Janssen A, Van den Heuvel LP, Morava E, Zeman J, Wevers RA, Lefeber DJ
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Journal Title
J Inherit Metab Dis
Volume: 34(4)
Pages: 901-906
Related Report
Peer Reviewed
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[Journal Article] Confirmation that SNPs in the high mobility group-A2 gene (HMGA2) are associated with adult height in the Japanese population; wide-ranging population survey of height-related SNPs in HMGA22011
Author(s)
Takeshita H, Fujihara J. Soejima M, Koda Y, Kimura-Kataoka K, Ono R, MuroT, Tongu M, Yuasa I, Iida, R, Ueki M, Yasuda T
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Journal Title
Electrophoresis
Volume: 32(14)
Pages: 1844-1851
Related Report
Peer Reviewed
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