Construction and Functional Analysis of Artificial Thylakoid Membranes
Project/Area Number |
24655120
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Functional materials chemistry
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Research Institution | Nagoya Institute of Technology |
Principal Investigator |
DEWA Takehisa 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (70335082)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 光合成膜 / 脂質二分子膜 / 人工チラコイド膜 / 積層膜 / 人工光合成膜 / 多層膜 / 光収穫系複合体 / バイセル / 多層膜構造 / チラコイド膜 / 光合成 |
Research Abstract |
Thylakoid and photosynthetic bacterial membranes possess three-dimensional multi-layered structures. These membranes efficiently captured transfer light energy. In order to reveal the three-dimensional structure-function relationship, we constructed multi-layered planar membrane containing photosynthesis antenna complex (LH2). Bicelles containing biotinyl lipids were tethered to a planar lipid bilayer via avidin-biotin interaction. Upon detergent removal, transformation from bicelle to planar membrane took place to form layered planar bilayer. The layered structure was observed by atomic force microscopy (AFM). Multi-layered membranes containing LH2 complex was also constructed by use of LH2-reconstituted bicelles. Thus, we successfully constructed artificial thylakoid membranes.
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Report
(3 results)
Research Products
(80 results)
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[Presentation] Molecular assembly of zinc chlorophyll derivatives by recombinant light-harvesting polypeptides with His-tag and immobilization on a gold electrode2013
Author(s)
S. Sakai, T. Noji, M. Kondo, T. Mizuno, T. Dewa, T. Ochiai, H. Yamakawa, S. Itoh, H. Hashimoto, M. Nango
Organizer
Light Harvesting Satellite Meeting 2013
Place of Presentation
Washington University, St. Louis
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[Presentation] 脂質二重膜への再構成系におけるLHCII, PSI, PSIIのエネルギー移動と光化学活性2012
Author(s)
野地 智康,天野 瑞貴,近藤 政晴,山川 壽伯,川上 恵典,沈 建仁,伊藤 繁,南後 守,出羽 毅久
Organizer
第61回高分子討論会
Place of Presentation
名古屋工業大学
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