Analysis of factors for enzymatic chitin solubilization
Project/Area Number |
25850077
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
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Research Institution | Ichinoseki National College of Technology |
Principal Investigator |
Nakagawa Yuko 一関工業高等専門学校, その他部局等, 准教授 (70435577)
|
Research Collaborator |
Vaaje-Kolstad Gustav
Eijsink Vincent G. H.
Mori Toshiaki
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 多糖バイオマス / 酵素分解 / ブースター / 分解促進 / LPMO / キチン / セルロース / Streptomyces griseus / 促進因子 / 組換え酵素 / 結合能 / 変異体 |
Outline of Final Research Achievements |
The lytic polysaccharide monooxygenases (LPMOs) have ability to boost the enzymatic conversion of recalcitrant polysaccharides. In the genome of Streotomyces griseus, 6 LPMO10 genes exist. We analyzed 5 SgLPMO10s and 4 of them showed ability to boost the enzymatic conversion towards alpha and beta-chitin. As regards to SgLPMO10F, we had published a paper in the FEBS Journal. SgLPMO10A, which is active on cellulose, showed novel activity as deacetylase. These data can be applied enzymatic conversion of polysaccharide biomass.
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Report
(5 results)
Research Products
(41 results)