A fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae

FEBS Lett. 2013 Mar 18;587(6):793-8. doi: 10.1016/j.febslet.2013.02.003. Epub 2013 Feb 14.

Abstract

The physiological roles of the thioredoxin isozymes in the yeast Saccharomyces cerevisiae were investigated using a novel FRET-based redox probe, Redoxfluor. After establishing responsiveness of the probe toward thioredoxin, we followed the fluorescence signal of Redoxfluor expressed in the yeast and found that one of the thioredoxin isozymes, Trx2, was required for maintaining the redox status when stationary culture of the organism was exposed to starvation and mild-heat stresses. The failure to maintain redox balance under the tested condition preceded decreased viability of the trx2 mutants, indicating the functional importance of the cytoplasmic thioredoxin in adaptation to environmental changes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques
  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Gene Deletion
  • Gene Expression
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Microbial Viability
  • Microscopy, Fluorescence
  • Oxidation-Reduction
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Starvation
  • Stress, Physiological
  • Thioredoxins / genetics
  • Thioredoxins / metabolism*

Substances

  • Fluorescent Dyes
  • Isoenzymes
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • TRX2 protein, S cerevisiae
  • Thioredoxins