Does electrical stimulation enhance the migration of transplanted cells in stroke model of rats? : Close up the mechanisms of electrical stimulation and search for the road to clinical application.
Project/Area Number |
25861277
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Neurosurgery
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Research Institution | Okayama University |
Principal Investigator |
Yasuhara Takao 岡山大学, 医歯(薬)学総合研究科, 講師 (50457214)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 細胞移植 / 電気刺激 / 細胞遊走能 / 細胞分化 / 脳梗塞 / パーキンソン病 / 幹細胞 / 再生医療 |
Outline of Final Research Achievements |
Electrical stimulation was performed through epidural electrode on infarcted cerebral cortex in a stroke model of rats using middle cerebral artery occlusion. Mesenchymal stem cells were transplanted into contralateral cerebral cortex after fluorescent labeling. After electrical stimulation for 2 weeks, cell proliferation, differentiation and migration were explored. The cell migration to the cerebral infarct was significantly enhanced by electrical stimulation, although there was no significant difference in cell proliferation and differentiation of transplanted mesenchymal stem cells. Bromodeoxyuridine labeling revealed that endogenous neural stem cells were more proliferated with more neural differentiations.
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Report
(4 results)
Research Products
(161 results)
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[Journal Article] Anti-high mobility group box 1 antibody exerts neuroprotection in a rat model of Parkinson's disease.2016
Author(s)
Sasaki, T., Liu, K., Agari, T., Yasuhara, T., Morimoto, J., Okazaki, M., Takeuchi, H., Toyoshima, A., Sasada, S., Shinko, A., Kondo, A., Kameda, M., Miyazaki, I., Asanuma, M., Borlongan, C.V., Nishibori, M. and Date, I.
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Journal Title
Exp. Neurol.
Volume: 275
Pages: 220-231
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Intra-arterial transplantation of allogeneic mesenchymal stem cells mounts neuroprotective effects in a transient ischemic stroke model in rats: Analyses of therapeutic time window and its mechanisms2015
Author(s)
Toyoshima A , Yasuhara T , Kameda M , Morimoto J , Takeuchi H , Wang F , Sasaki T , Sasada S , Shinko A , Wakamori T , Okazaki M , Kondo A , Agari T , Borlongan CV, Date I
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Journal Title
PLOS ONE
Volume: -
Issue: 6
Pages: e0127302-e0127302
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Infectious Spondylitis: Characteristics of Geriatric Patients and Significance of Surgery2014
Author(s)
Takao Yasuhara, Yuichi Takahashi, Yasukazu Hijikata,Tetsuya So, Shinji Kumamoto, Masayuki Nakahara, Takashi Tanoue, Akira Kusumegi, Takafumi Inoue, Koichi Ogawa,Hiroaki Manabe, Yasuyuki Miyoshi, Isao Date and Kenki Nishida
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Journal Title
J Spine Neurosurg
Volume: 3
Pages: 2-2
Related Report
Peer Reviewed
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[Journal Article] Therapeutic outcomes of transplantation of amniotic fluid-derived stem cells in experimental ischemic stroke2014
Author(s)
Tajiri N , Acosta SA, Gonzales-Portillo GS, Aguirre D, Reyes S, Lozano , Pabon MM, Dela Pena I, Ji X, Yasuhara T , Date I , Solomita MA, Antonucci I, Stuppia L, Kaneko Y, Borlongan CV
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Journal Title
Frontiers in Cellular Neuroscience
Volume: 8
Pages: 227-227
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Spinal cord stimulation exerts neuroprotective effects against experimental Parkinson's disease2014
Author(s)
Shinko A, Agari T, Kameda M, Yasuhara T, Kondo A, Tayra JT, Sato K, Sasaki T, Sasada S, Takeuchi H, Wakamori T, Borlongan CV, Date I
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Journal Title
PLoS One
Volume: 9
Issue: 7
Pages: e101468-e101468
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The neuroprotective and neurorescue effects of carbamylated erythropoietin Fc fusion protein (CEPO-Fc) in a rat model of Parkinson's disease2013
Author(s)
Tayra JT, Kameda M, Yasuhara T, Agari T, Kadota T, Wang F, Kikuchi Y, Liang H, Shinko A, Wakamori T, Vcelar B, Weik R, Date I
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Journal Title
Brain Research
Volume: 1502
Pages: 55-70
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Laterality of cervical disc herniation2013
Author(s)
Takahashi Y , Yasuhara T , Kumamoto S , Yoneda K , Tanoue T , Nakahara M , Inoue T , Hijikata Y , Lee T , Borlongan CV , Ogawa K , Nishida K
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Journal Title
Eur Spine J
Volume: 22
Pages: 178-182
Related Report
Peer Reviewed
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[Journal Article] Proximal vertebral body fracture after 4-level fusion using l1 as the upper instrumented vertebra for lumbar degenerative disease:report of 2 cases with literature review2013
Author(s)
Yasuhara T, Takahashi Y, Kumamoto S, Nakahara M, Yoneda K, Niimura T, Tanoue T, Kusumegi A, Sennari T, Hijikata Y, Manabe H, Miyoshi Y, Date I, Ogawa K, Nishida K
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Journal Title
Acta Med Okayama
Volume: 67
Pages: 197-202
Related Report
Peer Reviewed
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[Journal Article] In vivo animal stroke models: a rationale for rodent and non-human primate models2013
Author(s)
Tajiri N, Dailey T, Metcalf C, Mosley YI, Lau T, Staples M, van Loveren H, Kim SU, Yamashima T, Yasuhara T, Date I, Kaneko Y, Borlongan CV Transl
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Journal Title
Stroke Res
Volume: 4
Issue: 3
Pages: 308-321
DOI
Related Report
Peer Reviewed
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[Journal Article] Mannitol enhances therapeutic effects of intra-arterial transplantation of mesenchymal stem cells into the brain after traumatic brain injury2013
Author(s)
Okuma Y, Wang F, Toyoshima A, Kameda M, Hishikawa T, Tokunaga K, Sugiu K, Liu K, Haruma J, Nishibori M, Yasuhara T, Date I
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Journal Title
Neurosci Lett
Volume: 554
Pages: 156-61
DOI
Related Report
Peer Reviewed
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[Journal Article] Regenerative medicine for epilepsy : from basic research to clinical application2013
Author(s)
Yasuhara T, Agari T, Kameda M, Kondo A, Kuramoto S, Jing M, Sasaki T, Toyoshima A, Sasada S, Sato K, Shinko A, Wakamori T, Okuma Y, Miyoshi Y, Tajiri N, Borlongan CV, Date I
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Journal Title
Int J Mol Sci
Volume: 14
Issue: 12
Pages: 23390-401
DOI
Related Report
Peer Reviewed
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[Journal Article] Neuroprotective effects of liraglutide for stroke model of rats2013
Author(s)
Sato K, Kameda M, Yasuhara T, Agari T, Baba T, Wang F, Shinko A, Wakamori T, Toyoshima A, Takeuchi H, Sasaki T, Sasada S, Kondo A, Borlongan CV, Matsumae M, Date I
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Journal Title
Int J Mol Sci
Volume: 14
Issue: 11
Pages: 21513-24
DOI
Related Report
Peer Reviewed
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[Presentation] Encapsulated cell transplantation for central nervous system disorders2014
Author(s)
Yasuhara T, Kameda M, Agari T, Toyoshima A, Takeuchi H, Morimoto J, Kondo A, Shinko A, Sasada S, Sasaki T, Wakamori M, Okazaki M, Date I
Organizer
4th Neuroscience 2014
Place of Presentation
Washington, D.C
Year and Date
2014-11-19
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