Dendritic cell differentiation from myeloid neoplasm
Project/Area Number |
25461419
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
|
Research Institution | Osaka University |
Principal Investigator |
Mizuki Masao 大阪大学, 医学部附属病院, 准教授 (80283761)
|
Co-Investigator(Kenkyū-buntansha) |
KANAKURA Yuzuru 大阪大学, 医学系研究科, 教授 (20177489)
SHIBAYAMA Hirohiko 大阪大学, 医学系研究科, 講師 (60346202)
FUJITA Jiro 大阪大学, 医学系研究科, 助教 (90608720)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 樹状細胞 / 白血病関連遺伝子 / cDC / pDC / 組織球系腫瘍 / 白血病関連遺伝子変異 / DC / 白血病 / cDC / pDC |
Outline of Final Research Achievements |
We have established the dendritic cell (DC) differentiation system from murine hematopoietic stem cell fraction. We have shown that type I leukemia-related gene abnormalities except for FLT3 and ras, blocked DC differentiation and preferentially induced the differentiation of conventional DC (cDC) compared to plasmacytoid DC. In patients with TEL-PDGFRbeta positive chronic myelomonocytic leukemia, cDC subset was found to be derived from leukemic clone. In patients with acute myelogeneous leukemia showing spontaneous DC differentiation, there was a CD8+T cell subset, which reacted with leukemia-specific antigen, WT1, suggesting that leukemia-derived DC could present leukemia-specific antigens. Finally, origin-unknown soft tissue tumor was found to be histiocytic lineage, by whole exome sequensing which showed MAP2K1 mutation.
|
Report
(4 results)
Research Products
(9 results)
-
[Journal Article] Quantitative polymerase chain reaction analysis with allele-specific oligonucleotide primers for individual IgH VDJ regions to evaluate tumor burden in myeloma patients.2015
Author(s)
Sata H, Shibayama H, Maeda I, Habuchi Y, Nakatani E, Fukushima K, Fujita J, Ezoe S, Tadokoro S, Maeda T, Mizuki M, Kosugi S, Nakagawa M, Ueda S, Iida M, Tokumine Y, Azenishi Y, Mitsui H, Oritani K, Kanakura Y.
-
Journal Title
Exp Hematol.
Volume: 印刷中
Issue: 5
Pages: 374-381
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Maintenance of complete remission after allogeneic stem cell transplantation in leukemia patients treated with Wilms tumor 1 peptide vaccine2013
Author(s)
Maeda T, Hosen N, Fukushima K, Tsuboi A, Morimoto S, Matsui T, Sata H, Fujita J, Hasegawa K, Nishida S, Nakata J, Nakae Y, Takashima S, Nakajima H, Fujiki F, Tatsumi N, Kondo T, Hino M, Oji Y, Oka Y, Kanakura Y, Kumanogoh A, Sugiyama H
-
Journal Title
Blood Cancer J
Volume: 3
Issue: 8
Pages: e130-e130
DOI
Related Report
Peer Reviewed
-
-
-
-
-