Development of efficient differentiation methods using cytokine produced by human adipose-derived mesenchymal stem cells
Project/Area Number |
24791927
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Plastic surgery
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Research Institution | Kinki University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 脂肪由来間葉系幹細胞 / 酸化ストレス / 神経伸張 / 再生医療 / 細胞治療 / p38 MAPキナーゼ / ヒト脂肪由来幹細胞 / 神経再生 / 分化誘導 / 分化誘導シグナル |
Research Abstract |
In this study, we examined the role of oxidative stress on human adipose-derived multilineage progenitor cells (hADMPCs) in neurite outgrowth in PC12. We found that glutathione depletion, followed by accumulation of reactive oxygen species, stimulates the expression of BMP2 and FGF2 in hADMPCs. Addition of N-acetylcysteine, a precursor of the intracellular antioxidant glutathione, suppressed the BSO-mediated upregulation of BMP2 and FGF2. Moreover, BSO treatment caused phosphorylation of p38 MAPK in hADMPCs. Inhibition of p38 MAPK was sufficient to suppress BMP2 and FGF2 expression, while this expression was significantly upregulated by overexpression of a constitutively active form of MKK6, which is an upstream molecule from p38 MAPK. Thus, transplantation of hADMPCs into neurodegenerative lesions such as stroke and Parkinson's disease, in which the transplanted hADMPCs are exposed to oxidative stress, can be the basis for simple and safe therapies.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Hypoxia increases Gefitinib-resistant lung cancer stem cells through the activation of insulin-like growth factor 1 receptor2014
Author(s)
Murakami A, Takahashi F, Nurwidya F, Kobayashi I, Minakata K, Hashimoto M, Nara T, Kato M, Kajima K, Shimada N, Iwakami S, Moriyama M, Moriyama H, Koizumi F, Takahashi K
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Journal Title
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Peer Reviewed
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[Journal Article] Hypoxia induces Gefitinib resistance in non-small cell lung cancer with both mutant and wild-type epidermal growth factor receptors2012
Author(s)
Minakata K, Takahashi F, Nara T, Hashimoto M, Tajima K, Murakami A, Nurwidya F, Yae S, Koizumi F, Moriyama H, Seyama K, Nishio K, Takahashi K
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Journal Title
Cancer Sci
Volume: 103(11)
Pages: 1946-54
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Peer Reviewed
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[Presentation] Human adipose tissue-derived multilineage progenitor cells exposed to oxidative stress induce neurite outgrowth in PC12 cells through p38 MAPK signaling.2012
Author(s)
Hiroyuki Moriyama, Nomura, Chiaki Sone, Mariko Moriyama, Ayaka Ueda, Ryousuke Nishibata, Kouji Fukase, Hanayuki Okura, Akifumi Matsuyama, Takao Hayakawa.
Organizer
The 34th annual meeting of the molecular biology society of Japan
Place of Presentation
Fukuoka, Japan
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[Presentation] 化ストレスを負荷したヒト脂肪組織由来多系統前駆細胞を介する神経分化誘導メカニズムの解明.
Author(s)
西端勇介,森山麻里子,西川彩菜,深瀬尭哉,福井承子,本庄清貴,上田彩加,大倉華雪,松山晃文,早川堯夫,森山博由.
Organizer
第62回 日本薬学会近畿支部総会・大会
Place of Presentation
兵庫, 日本
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