Explore of a novel molecule regulating Langerhans cells by visualizing method
Project/Area Number |
16K15495
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Endocrinology
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Research Institution | Kyoto University |
Principal Investigator |
NAKAO KAZUWA 京都大学, 医学研究科, 特任教授 (00172263)
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Co-Investigator(Renkei-kenkyūsha) |
KANDA HAJIME 京都大学, 大学院医学研究科, 客員研究員 (00724535)
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Project Period (FY) |
2016-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
|
Keywords | 膵ランゲルハンス島 / ゼブラフィッシュ / アルファ細胞 / ベータ細胞 / デルタ細胞 / 共焦点レーザー顕微鏡 / フローサイトメトリー / セルソーター / 糖尿病 / 生体分子 / 遺伝子 / 細胞・組織 / 動物 |
Outline of Final Research Achievements |
We developed a triple transgenic zebrafish expressing three types of fluorescent reporter protein for alpha cell, beta cell and delta cell of Langerhans islets of pancreas. Our research purpose was elucidation of molecular mechanism regulating volume and ratio of alpha, beta and delta cell in a Langerhans islet. Imaging analysis of 5dpf, 7dpf and 9dpf triple transgenic zebrafish was performed, and we found that the number of alpha cell was significantly increased than beta cells from 5dpf to 7dpf. To analyze molecular mechanism to regulate alpha cell and beta cell ratio, we developed sorting methods to analyze isolated cells of Langerhans of islet by Flow cytometry. Our result and developed methods have a potential to contribute future researches of Langerhans islet cells.
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Report
(2 results)
Research Products
(13 results)
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[Journal Article] MiR30-GALNT1/2 Axis-Mediated Glycosylation Contributes to the Increased Secretion of Inactive Human Prohormone for Brain Natriuretic Peptide (proBNP) From Failing Hearts.2017
Author(s)
Nakagawa Y, Nishikimi T, Kuwahara K, Fujishima A, Oka S, Tsutamoto T, Kinoshita H, Nakao K, Cho K, Inazumi H, Okamoto H, Nishida M, Kato T, Fukushima H, Yamashita JK, Wijnen WJ, Creemers EE, Kangawa K, Minamino N, Nakao K, Kimura T.
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Journal Title
J Am Heart Assoc.
Volume: 6
Issue: 2
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Endothelium-derived C-type natriuretic peptide contributes to blood pressure regulation by maintaining endothelial integrity.2017
Author(s)
Nakao K, Kuwahara K, Nishikimi T, Nakagawa Y, Kinoshita H, Minami T, Kuwabara Y, Yamada C, Yamada Y, Tokudome T, Nagai-Okatani C, Minamino N, Nakao YM, Yasuno S, Ueshima K, Sone M, Kimura T, Kangawa K, Nakao K
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Journal Title
Hypertension.
Volume: 69
Issue: 2
Pages: 286-296
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] MOB1-YAP1/TAZ-NKX2.1 axis controls bronchioalveolar cell differentiation, adhesion and tumour formation. Oncogene2017
Author(s)
Otsubo K, Goto H, Nishio M, Kawamura K, Yanagi S, Nishie W, Sasaki T, Maehama T, Nishina H, Mimori K, Nakano T, Shimizu H, Mak TW, Nakao K, Nakanishi Y, Suzuki A
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Journal Title
Oncogene
Volume: 印刷中
Issue: 29
Pages: 4201-4211
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Ablation of the N-type calcium channel ameliorates diabetic nephropathy with improved glycemic control and reduced blood pressure.2016
Author(s)
Ohno S, Yokoi H, Mori K, Kasahara M, Kuwahara K, Fujikura J, Naito M, Kuwabara T, Imamaki H, Ishii A, Saleem MA, Numata T, Mori Y, Nakao K, Yanagita M, Mukoyama M
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 27192-27192
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Overall safety and efficacy of high-dose and low-dose intravenous glucocorticoid therapy in patients with moderate-to-severe active Graves’ ophthalmopathy2016
Author(s)
Ueda-Sakane Y, Kanamoto N, Fushimi Y, Tanaka-Mizuno S, Yasuno S, Miura M, Sone M, Yasoda A, Okada T, Togashi K, Nakao K, Inagaki N
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Journal Title
Endocrine Journal
Volume: 63
Issue: 8
Pages: 703-714
DOI
NAID
ISSN
0918-8959, 1348-4540
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Brain-specific natriuretic peptide receptor-B deletion attenuates high-fat diet-induced visceral and hepatic lipid deposition in mice.2016
Author(s)
Yamashita Y, Yamada-Goto N, Katsuura G, Ochi Y, Kanai Y, Miyazaki Y, Kuwahara K, Kanamoto N, Miura M, Yasoda A, Ohinata K, Inagaki N, Nakao K.
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Journal Title
Peptides
Volume: 81
Pages: 38-50
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Impaired adipogenic capacity in induced pluripotent stem cells from lipodystrophic patients with BSCL2 mutations2016
Author(s)
1.E. Mori, J. Fujikura, M. Noguchi, K. Nakao, M. Matsubara, M. Sone, D. Taura, T. Kusakabe, K. Ebihara, T. Tanaka, K. Hosoda, K. Takahashi, I. Asaka, N. Inagaki, K. Nakao
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Journal Title
Metablism
Volume: 65
Issue: 4
Pages: 543-556
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Book] Academic Press2016
Author(s)
錦見俊雄、桑原宏一郎、中川靖章、寒川賢治、中尾一和
Publisher
Endocrinology of the Heart in Health and Disease: Integrated, Cellular, and Molecular Endocrinology of the Heart
Related Report