Development of fluorometric potassium ion imaging reagent in a cell
Project/Area Number |
22550154
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Chemistry related to living body
|
Research Institution | Kyushu Institute of Technology |
Principal Investigator |
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | ナノバイオ / 分子認識 / 細胞・組織 / 生体分子 / 蛍光プローブ / Gリッチオリゴヌクレオチド / 4本鎖DNA / FRET / カリウム / ナトリウム / G-カルテット / オリゴヌクレオチド / カリウムイオン / 蛍光イメージング / 4本鎖 / 細胞 / ペプチド / 細胞内カリウムイオン / アポトーシス / Kチャネル / G-リッチオリゴヌクレオチド / 核排出シグナルペプチド |
Research Abstract |
In this project, we successful synthesized a fluorometric potassiumdetecting reagent, PSO-5, consisted of thrombin binding aptamer (TBA) carrying FAM at the 5'-end conjugated with a peptide carrying TAMRA and biotin at the middle and at its C-terminus, respectively. K^+ in the cell was visualized based on the FRET ratio of the ternary complex of streptavidin with PSO-5 and biotinylated nuclear export signal peptide. Change of FRET signal of PSO-5 was observed that indicated a decrease in K^+ concentration in the cell upon addition of apoptosis-inducing drug to monitor K^+ efflux from the cell.
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Report
(4 results)
Research Products
(46 results)