Development of the microscopy for optical sectioning chemiluminescence imaging
Project/Area Number |
25650052
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biophysics
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Research Institution | Osaka University |
Principal Investigator |
Arai Yoshiyuki 大阪大学, 産業科学研究所, 助教 (20444515)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | イメージング / 化学発光 / 3次元 / 顕微鏡 / 球面収差 |
Outline of Final Research Achievements |
Chemiluminescence imaging enable us excitation light free imaging since the chemiluminescencent probes can emit the light by the reaction with the chemiluminescent substrates. However, three dimensional imaging cannot be done because the system do not use illumination optical system. Here I develop the microscopy by utilizing the spherical aberration of the optical lens for three dimensional chemiluminescence imaging. Although my system could not be used for the extraction of optical plane of chemiluminescence image, the system might have the possibility for other fluorescence imaging system.
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Report
(5 results)
Research Products
(4 results)
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[Journal Article] Improved orange and red Ca2+ indicators and photophysical considerations for optogenetic applications.2013
Author(s)
Wu JH, Liu L, Matsuda T, Zhao YG, Rebane A, Drobijev M, Chang YF Araki S, Arai Y, March K, Sagou K, Miyata T, Nagai T, Li WH, Campbell R,
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Journal Title
ACS Chen. Neurosci.
Volume: 4
Issue: 6
Pages: 963-972
DOI
Related Report
Peer Reviewed
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