Embryological analysis of mouse Multilineage-differentiating stress enduring (Muse) cells.
Project/Area Number |
24790188
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General anatomy (including Histology/Embryology)
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Research Institution | Tohoku University |
Principal Investigator |
WAKAO Shohei 東北大学, 医学(系)研究科(研究院), 助教 (80511948)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 多能性幹細胞 |
Outline of Final Research Achievements |
Muse cells can be isolated from mesenchymal stem cells by FACS as cells positive for stage-specific embryonic antigen-3 (SSEA-3), a marker for undifferentiated human pluripotent stem cells. However, SSEA-3 is a glycolipid, it is difficult to embryological analysis. In order to search for the new marker, Muse cells were subjected to FACS analysis using various antibodies. From these results, it was suggested that Cripto-1 was a new marker in substitution for SSEA-3. Therefore, we constructed several lines of transgenic mice expressing GFP under the control of Cripto-1 promoter and subjected to histological analysis of these mice connective tissue. The GFP and SSEA-3 double positive cells were located sparsely in the connective tissue of the hypodermis and do not associated with particular structures such as blood vessels. These transgenic mice will be useful tool for future investigation of embryological analysis of Muse cells.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Autologous mesenchymal stem cell-derived dopaminergic neurons function in parkinsonian macaques2013
Author(s)
Hayashi T, Wakao S, Kitada M, Ose T, Watabe H, Kuroda Y, Mitsunaga K, Matsuse D, Shigemoto T, Ito A, Ikeda H, Fukuyama H, Onoe H, Tabata Y, Dezawa M
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Journal Title
J Clin Invest
Volume: 123(1)
Issue: 1
Pages: 272-284
DOI
Related Report
Peer Reviewed
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[Journal Article] Transplantation of bone marrow stromal cells-derived neural precursor cells ameliorates deficits in a rat model of complete spinal cord transection.2012
Author(s)
Aizawa-Kohama M, Endo T, Kitada M, Wakao S, Sumiyoshi A, Matsuse D, Kuroda Y, Morita T, Riera JJ, Kawashima R, Tominaga T, Dezawa M
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Journal Title
Cell Transplantation
Volume: -
Related Report
Peer Reviewed
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[Book] 最新医学2014
Author(s)
若尾昌平、串田良祐、出澤真理
Total Pages
745
Publisher
最新医学社
Related Report