Promotion of hematogenous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-2

  • Authors:
    • Emi Segawa
    • Hiromitsu Kishimoto
    • Kazuki Takaoka
    • Kazuma Noguchi
    • Susumu Hashitani
    • Kazunari Sakurai
    • Masahiro Urade
  • View Affiliations

  • Published online on: September 1, 2010     https://doi.org/10.3892/or_00000915
  • Pages: 733-739
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Abstract

To understand the role of cyclooxygenase (COX)-2 in metastatic potential of oral cancer, COX-2 overexpressing KB/COX-2 cells were inoculated orthotopically into the masseter muscle or injected into the left cardiac ventricle of nude mice. KB/COX-2 showed about 4-fold increase of COX-2 protein expression as compared to KB/Neo which was a mock transfected control. In orthotopic inoculation, metastasis to the regional lymph nodes occurred in 2 out of 15 mice, and metastasis to the lung in 3 out of 15 mice. On the other hand, in intra-cardiac injection, hematogenous metastasis to the lung and bone occurred in 8 out of 10 mice in KB/COX-2, but no metastasis occurred except for only one metastasis to the femur bone out of 10 mice in KB/Neo. Treatment of KB/COX-2 with COX-2 small interfering RNA (siRNA) inhibited the colony formation but not cell growth in vitro, and suppressed tumorigenicity and hematogenous metastasis in nude mice. When expression of adhesion molecules such as E-cadherin, α-catenin, β-catenin and CD44 was examined, there was no difference in α- and β-catenin between the cells. However, expression of E-cadherin was detected in KB/Neo, but not in KB/COX-2. In contrast, expression of CD44 was markedly increased in KB/COX-2 as compared to KB/Neo. Treatment with COX-2 siRNA resulted in suppression of CD44 expression and detectable expression of E-cadherin in KB/COX-2. These findings suggested that overexpression of COX-2 increased hematogenous metastasis, at least in KB cells, via down-regulating E-cadherin and up-regulating CD44 expression.

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September 2010
Volume 24 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Segawa E, Kishimoto H, Takaoka K, Noguchi K, Hashitani S, Sakurai K and Urade M: Promotion of hematogenous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-2 . Oncol Rep 24: 733-739, 2010
APA
Segawa, E., Kishimoto, H., Takaoka, K., Noguchi, K., Hashitani, S., Sakurai, K., & Urade, M. (2010). Promotion of hematogenous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-2 . Oncology Reports, 24, 733-739. https://doi.org/10.3892/or_00000915
MLA
Segawa, E., Kishimoto, H., Takaoka, K., Noguchi, K., Hashitani, S., Sakurai, K., Urade, M."Promotion of hematogenous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-2 ". Oncology Reports 24.3 (2010): 733-739.
Chicago
Segawa, E., Kishimoto, H., Takaoka, K., Noguchi, K., Hashitani, S., Sakurai, K., Urade, M."Promotion of hematogenous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-2 ". Oncology Reports 24, no. 3 (2010): 733-739. https://doi.org/10.3892/or_00000915