DNA Damage Recognition Proteins Localize along Heavy Ion Induced Tracks in the Cell Nucleus

  • TAKAHASHI Akihisa
    Departments of Biology, School of Medicine, Nara Medical University
  • YAMAKAWA Nobuhiro
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Nara Medical University
  • KIRITA Tadaaki
    Departments of Oral and Maxillofacial Surgery, School of Medicine, Nara Medical University
  • OMORI Katsunori
    Department of Space Biology and Microgravity Sciences, Japan Aerospace Exploration Agency
  • ISHIOKA Noriaki
    Department of Space Biology and Microgravity Sciences, Japan Aerospace Exploration Agency
  • FURUSAWA Yoshiya
    Heavy-ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences
  • MORI Eiichiro
    Departments of Biology, School of Medicine, Nara Medical University
  • OHNISHI Ken
    Departments of Biology, School of Medicine, Nara Medical University
  • OHNISHI Takeo
    Departments of Biology, School of Medicine, Nara Medical University

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Abstract

To identify the repair dynamics involved in high linear energy transfer (LET) radiation-induced DNA damage, phospho-H2AX (γH2AX) foci formation was analyzed after cellular exposure to iron ions (Fe-ions, 500 MeV u-1, 200 KeV μm-1). The foci located at DNA damage sites were visualized using immunocytochemical methods. Since H2AX is phosphorylated at sites of radiation-induced double strand breaks (DSB), γH2AX foci were used to detect or illuminate tracks formed by DSB after exposure to various doses of ionizing radiation. Additional DSB-recognition proteins such as ATM phospho-serine 1981, DNA-PKcs phospho-threonine 2609, NBS1 phospho-serine 343 and CHK2 phospho-threonine 68 all co-localized with γH2AX at high LET radiation induced DSB. In addition, Fe-ion induced foci remained for longer times than X-radiation induced foci. These findings suggest that Fe-ion induced damage is repaired more slowly than X-radiation induced damage, possibly because Fe-ion induced damage or lesions are more complex or extensive. Antibodies for all these phosphorylated DNA DSB recognition proteins appear to be very effective for the detection and localization of DSB.

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