Characterization of Olig2 expression during cerebellar development
Section snippets
Olig2 is transiently expressed in the cerebellar VZ during specific developmental stages
To determine the expression period of Olig2 in the cerebellar VZ, we performed immunohistochemistry on embryonic cerebella at several developmental stages. Olig2 expression was detectable both in the VZ and parenchyma of the cerebellum. Olig2 in the VZ was detectable only during developmental stages E11.5 ∼ E13.5 (Fig. 1A–F). This period coincides with the peak period of Purkinje cell production (Hashimoto and Mikoshiba, 2003, Miale and Sidman, 1961, Sudarov et al., 2011). This result is
Discussion
In this study, we analyzed the expression pattern of Olig2 in the cerebellar VZ during early development. Olig2 was found to be expressed in Ptf1a-positive cells (progenitors for cerebellar GABAergic neurons) during a specific period around E11.5 ∼ E13.5, stages important for the generation of Purkinje cells (Hashimoto and Mikoshiba, 2003, Miale and Sidman, 1961, Sudarov et al., 2011).
Co-expression analysis of Ki67 and BrdU with Olig2 indicated that some populations of Olig2-positive cells were
Animals and immunohistochemistry
All animal experiments in this study have been approved by the Animal Care and Use Committee of the National Institute of Neuroscience, Japan. Embryos were fixed with 4% paraformaldehyde in PBS. Embryos embedded in OCT compound were cryosectioned at a thickness of 14 μm. Sections were treated with 0.5% BSA in PBS containing 0.2% Triton X-100 (blocking buffer) at room temperature for 1 h and subsequently incubated with antibodies listed below in blocking buffer at 4 °C for 20 h. Specimens were
Acknowledgement
We would like to thank Dr. Ruth Yu for comments. This work is supported by grants from the MEXT, Naito foundation, Intramural Research Grants (24-12, 25-3) for Neurological and Psychiatric Disorders of NCNP and Health Science Research Grant for Research on Psychiatric and Neurological Diseases and Mental Health (H23-001) from the Japanese Ministry of Health, Labor and Welfare.
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