Elsevier

Developmental Biology

Volume 381, Issue 2, 15 September 2013, Pages 401-410
Developmental Biology

Atoh1 directs hair cell differentiation and survival in the late embryonic mouse inner ear

https://doi.org/10.1016/j.ydbio.2013.06.022Get rights and content
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Highlights

  • Late Atoh1 expression regulates cochlear hair cell survival and then differentiation.

  • In the utricle, late Atoh1 expression regulates differentiation but not survival.

  • Atoh1 maintains expression of Barhl1 and Gfi1 but not Pou4f3 or other hair cell markers.

Abstract

Atoh1 function is required for the earliest stages of inner ear hair cell development, which begins during the second week of gestation. Atoh1 expression in developing hair cells continues until early postnatal ages, but the function of this late expression is unknown. To test the role of continued Atoh1 expression in hair cell maturation we conditionally deleted the gene in the inner ear at various embryonic and postnatal ages. In the organ of Corti, deletion of Atoh1 at E15.5 led to the death of all hair cells. In contrast, deletion at E16.5 caused death only in apical regions, but abnormalities of stereocilia formation were present throughout the cochlea. In the utricle, deletion at E14.5 or E16.5 did not cause cell death but led to decreased expression of myosin VIIa and failure of stereocilia formation. Furthermore, we show that maintained expression of Barhl1 and Gfi1, two transcription factors implicated in cochlear hair cell survival, depends upon continued Atoh1 expression. However, maintained expression of Pou4f3 and several hair cell-specific markers is independent of Atoh1 expression. These data reveal novel late roles for Atoh1 that are separable from its initial role in hair cell development.

Keywords

Hearing
Cochlea
Development
Vestibular system
Differentiation

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