Characterization of PRL as a redox-sensing molecule
Project/Area Number |
22770195
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Cell biology
|
Research Institution | Osaka University |
Principal Investigator |
FUNATO Yosuke 大阪大学, 微生物病研究所, 助教 (60505775)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 細胞内情報伝達 / PRL / マグネシウム / がん転移 / MEP50 / PSMC6 |
Research Abstract |
I searched for a novel interacting protein of PRL, a protein tyrosine phosphatase involved in tumor metastasis, and identified MagEx as a major binding partner of PRL. MagEx interacts with PRL in a redox-dependent manner. Functional analyses revealed that MagEx stimulates Mg^<2+>-efflux. Overexpression of PRL inhibits the MagEx-dependent Mg^<2+>-efflux, which is restored by stimulating the cells with hydrogen peroxide. Mg^<2+>-efflux by MagEx results in suppression of Akt/ mTOR signaling, which is cancelled by co-expression of PRL. Furthermore, experimental metastasis analyses in mice reveal that RNAi-mediated knockdown of MagEx resulted in increased metastatic nodules in lungs. Collectively, these results indicate that MagEx is the molecular target for PRL.
|
Report
(3 results)
Research Products
(18 results)
-
-
-
-
[Journal Article] Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 and growth cone collapse2011
Author(s)
Morinaka, A., Yamada, M., Itofusa, R., Funato, Y., Yoshimura, Y., Nakamura, F., Yoshimura, T., Kaibuchi, K., Goshima, Y., Hoshino, M., Kamiguchi, H., and Miki, H.
-
Journal Title
Related Report
Peer Reviewed
-
-
[Journal Article] Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 and growth cone collapse2011
Author(s)
Morinaka A, Yamada M, Itofusa R, Funato Y, Yoshimura Y, Nakamura F, Yoshimura T, Kaibuchi K, Goshima Y, Hoshino M, Kamiguchi H, Miki H
-
Journal Title
Science Signaling
Volume: 4(179)
Issue: 170
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive Dishevelled protein2010
Author(s)
Funato, Y., Terabayashi, T., Sakamoto, R., Okuzaki, D., Ichise, H., Nojima, H., Yoshida, N., and Miki H.
-
Journal Title
Curr. Biol
Volume: 20
Pages: 1945-1952
Related Report
Peer Reviewed
-
[Journal Article] Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of flightless-I to myeloid differentiation primary response gene2010
Author(s)
#Hayashi, T., # Funato, Y., Terabayashi, T., Morinaka, A., Sakamoto, R., Ichise, H., Fukuda, H., Yoshida, N., and Miki, H.
-
Journal Title
J. Biol. Chem
Volume: 285
Pages: 18586-18593
Related Report
Peer Reviewed
-
-
-
-
-
-
-