Induction of bone repair in rat calvarial defects using a combination of hydroxyapatite with phosphatidylserine liposomes
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- Hatakeyama Junko
- Section of Operative Dentistry and Endodontology, Fukuoka Dental College
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- Anan Hisashi
- Section of Operative Dentistry and Endodontology, Fukuoka Dental College
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- Hatakeyama Yuji
- Section of Molecular Cell Biology and Oral Anatomy, Fukuoka Dental College
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- Matsumoto Noriyoshi
- Section of Operative Dentistry and Endodontology, Fukuoka Dental College
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- Takayama Fumiko
- Departments of Aging Science and Pharmacology, Faculty of Dental Science, Kyushu University
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- Wu Zhou
- Departments of Aging Science and Pharmacology, Faculty of Dental Science, Kyushu University
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- Matsuzaki Etsuko
- Section of Operative Dentistry and Endodontology, Fukuoka Dental College
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- Minakami Masahiko
- Section of Operative Dentistry and Endodontology, Fukuoka Dental College
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- Izumi Toshio
- Section of Operative Dentistry and Endodontology, Fukuoka Dental College
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- Nakanishi Hiroshi
- Departments of Aging Science and Pharmacology, Faculty of Dental Science, Kyushu University
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Abstract
<p>Phosphatidylserine (PS)―normally present on the inner leaflet of the plasma membrane―translocates to the outer leaflet at an early stage of apoptosis. PS-containing liposomes (PSLs) can mimic the effect of apoptotic cells in inducing the secretion of prostaglandin E2 from phagocytes and inhibiting the maturation of dendritic cells and osteoclast precursors. The present study attempted to evaluate the effect of calcium phosphate (in the form of hydroxyapatite [HAP]) in the presence or absence of PSLs for repair of rat calvarial bone defects. The defects, each 5 mm in diameter, were created in the calvaria parietal bone of 8-week-old Wistar rats and subjected to one of the following treatments: no augmentation (Sham), HAP alone, or a mixture of HAP and PSL (HAP+PSL). Micro-computed tomography data showed that the HAP+PSL complexes promoted greater bone regeneration in comparison with either the Sham procedure or HAP alone at 4 and 8 weeks after implantation. The regeneration of calvarial bone defects induced by PSLs was mediated partly through upregulation of the osteogenic marker Alkaline Phosphatase, Type I collagen, osteocalcin, Runx2, and Osterix mRNAs. These data are the first to show that PSLs can influence bone regeneration by regulating osteoblast differentiation.</p>
Journal
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- Journal of Oral Science
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Journal of Oral Science 61 (1), 111-118, 2019
Nihon University School of Dentistry
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Details
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- CRID
- 1390282763116870528
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- NII Article ID
- 130007626633
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- ISSN
- 18804926
- 13434934
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- PubMed
- 30918207
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed