Functional analysis to reveal the role of GATA2 during hematopoietic differentiation based on disease-specific iPS cells
Project/Area Number |
16K19564
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Tohoku University |
Principal Investigator |
HATTA SHUNSUKE 東北大学, 医学系研究科, 非常勤講師 (80772194)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 血液内科学 / GATA2 / iPS細胞 |
Outline of Final Research Achievements |
We aimed to establish induced pluripotent stem (iPS) cells using episomal vectors based on patient-derived mesenchymal stem cells. So far, we have not succeeded to establish the iPS cells, presumably due to increased genome instability caused by GATA-2 mutation. Alternatively, we established iPS cells from patient with X-linked sidroblastic anemia (XLSA). When the iPS cells were differentiated into erythroid cells by co-culturing with stromal cells, the erythroblasts exhibited ringed sideroblasts and abnormal iron deposition in the mitochondria, confirmed by electron microscopy. Further phenotypic analyses would lead to the better understanding of the molecular basis of XLSA as well as the establishment of novel therapeutic strategies.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Successful Cord Blood Stem Cell Transplantation for Primary Cutaneous CD8-positive Aggressive Epidermotropic Cytotoxic T-cell Lymphoma Complicated with Cerebral Infiltration2018
Author(s)
Ichikawa S, Fukuhara N, Hatta S, Himuro M, Katsushima H, Nasu K, Ono K, Inokura K, Kobayashi M, Onishi Y, Fujii H, Ishizawa K, Ichinohasama R, Harigae H.
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Journal Title
Internal Medicine
Volume: 57
Issue: 14
Pages: 2051-2055
DOI
NAID
ISSN
0918-2918, 1349-7235
Year and Date
2018-07-15
Related Report
Peer Reviewed / Open Access
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[Journal Article] Dynamics of absorption, metabolism, and excretion of 5-aminolevulinic acid in human intestinal Caco-2 cells.2017
Author(s)
Saito K, Fujiwara T, Ota U, Hatta S, Ichikawa S, Kobayashi M, Okitsu Y, Fukuhara N, Onishi Y, Ishizuka M, Tanaka T, Harigae H
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Journal Title
Biochemistry and Biophysics Reports
Volume: 11
Pages: 105-111
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Impact of TET2 deficiency on iron metabolism in erythroblasts.2017
Author(s)
Inokura K, Fujiwara T, Saito K, Iino T, Hatta S, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Shimoda K, Harigae H
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Journal Title
Exp Hematol.
Volume: -
Pages: 56-67
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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