Role of GATA-2 in bone marrow-derived mesenchymal stem cells
Project/Area Number |
25860776
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Tohoku University |
Principal Investigator |
Okitsu Yoko 東北大学, 大学病院, 助教 (80451558)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | GATA-2 / 間葉系幹細胞 / GATA2 / 再生不良性貧血 |
Outline of Final Research Achievements |
The bone marrow microenvironment comprises multiple cell niches derived from bone marrow mesenchymal stem cells (BM-MSCs). However, the molecular mechanism of BM-MSC differentiation is poorly understood. The transcription factor GATA2 is indispensable for hematopoietic stem cells (HSCs) function as well as other hematopoietic lineages, suggesting that it may maintain BM-MSCs in immature state and also contribute to their differentiation. To explore this possibility, we established BM-MSCs from human bone marrow. GATA2 loss- and gain-of-function analyses based on human BM-MSCs confirmed that decreased and increased GATA2 expression accelerated and suppressed BM-MSCs differentiation to adipocytes, respectively. When GATA2 knockdowned BM-MSCs were cocultured with CD34+ cells, HSC frequency and colony formation decreased, indicating that decreased GATA2 expression induced BM-MSC differentiation, particularly into adipocytes, leading to the decreased hematopoietic supporting capacity.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] GATA2 regulates differentiation of bone marrow-derived mesenchymal stem cells.2014
Author(s)
Kamata M, Okitsu Y, Fujiwara T, Kanehira M, Nakajima S, Takahashi T, Inoue A, Fukuhara N, Onishi Y, Ishizawa K, Shimizu R, Yamamoto M, Harigae H.
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Journal Title
Haematologica
Volume: 99
Issue: 11
Pages: 1686-1696
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Role of GATA2 in the differentiation of bone marrow-derived mesenchymal stem cells2014
Author(s)
Kamata M, Okitsu Y, Fujiwara T, Nakajima S, Takahashi T, Inoue A, Fukuhara N, Onishi Y, Ishizawa K, Shimizu R, Yamamoto M, Harigae H.
Organizer
The 76th Annual Meeting of the Japanese Society of Hematology
Place of Presentation
Osaka International Convention Center
Year and Date
2014-10-31 – 2014-11-02
Related Report