Journal of Biological Chemistry
Plant BiologyThe Crystal Structure of the Plant Small GTPase OsRac1 Reveals Its Mode of Binding to NADPH Oxidase*
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This work was supported in part by Grants-in-aid for science research (to I. O., Y. K., C. K., and K. S.), the Targeted Proteins Research Program (to C. K. and K. S.), a Ministry of Agriculture, Forestry and Fisheries Genomics for Agricultural Innovation grant (to K. S.), Grants-in-aid for JSPS Fellows (to K. K. and M. N.), and the Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
The atomic coordinates and structure factors (code 4U5X) have been deposited in the Protein Data Bank (http://wwpdb.org/).
This article contains supplemental Figs. S1–S6.
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This work is dedicated to Ko Shimamoto, who died September 28, 2013.
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These authors contributed equally to this work.
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The abbreviations used are:
- ROS
reactive oxygen species
- GEF
guanine nucleotide exchange factor
- CA
constitutively activated
- DN
dominant-negative
- GMPPNP
guanosine 5′-(β,γ-imido)triphosphate
- BisTris
2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
- PDB
Protein Data Bank
- HSQC
heteronuclear single quantum coherence
- GTPγS
guanosine 5′-O-(thiotriphosphate).