Site selective cellular analysis by solid-state NMR
Project/Area Number |
26291029
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Biophysics
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Research Institution | Osaka University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
KOJIMA Chojiro 大阪大学, 蛋白質研究所, 准教授 (50333563)
MATSUKI Yoh 大阪大学, 蛋白質研究所, 助教 (70551498)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
|
Keywords | 核磁気共鳴 / 細胞生物学 / 構造生物学 / 細胞内分子構造 / 常磁性造影剤 / 細胞内局在イメージング / 生物物理学 / 生物物理 |
Outline of Final Research Achievements |
The thermal equilibrium NMR signal intensities give the quantitative information for all the cellular molecules at low temperatures where molecular motions are suppressed. The molecules for a single cell were counted by combined use of optical and electron microscope analyses, which enabled us to trace the biosynthetic processes by using the 13C labeled media without the purification. Applications of our magic-angle-spinning DNP NMR spectrometer with a closed-cycle He-gas system at low temperature 30 K and 16.4 T are also shown.
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Report
(5 results)
Research Products
(39 results)
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[Journal Article] The crystal structure of the plant small GTPase OsRacl reveals its mode of binding to NADPH oxidase.2015
Author(s)
Kosami KI, Ohki I, Nagano M, Furuita F, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
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Journal Title
The Journal of Biological Chemistry
Volume: 289
Issue: 41
Pages: 28569-78
DOI
Description
(in press)(印刷中)
Related Report
Peer Reviewed
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[Journal Article] Active-Site Structure of Thermophilic Foc-Subunit Ring in Membranes Elucidated by Solid-State NMR2014
Author(s)
Kang S, Todokoro Y, Yumen I, Shen B, Iwasaki I, Suzuki T, Miyagi A, Morikawa K, Yoshida M, Fujiwara T, Akutsu H
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Journal Title
Biophys J
Volume: 106
Issue: 2
Pages: 390-398
DOI
Related Report
Peer Reviewed / Open Access
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