Measles virus infection of epitherial cells
Project/Area Number |
22790445
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Virology
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
TAHARA Maino 国立感染症研究所, ウイルス第三部, 主任研究官 (10572109)
|
Project Period (FY) |
2010-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | 麻疹ウイルス / ウイルス学 / ワクチン / 上皮細胞 / 極性上皮細胞 / 受容体 |
Research Abstract |
Monoclonal antibodies (MAbs) recognizing distinct epitopes on the H protein were characterized using recombinant MVs. We identified the location of five epitopes on the H protein structure. Our data in the present study demonstrated that the H protein of MV possesses at least two conserved effective neutralizing epitopes. One, which is a previously recognized epitope, is located near the receptor-binding site (RBS), whereas the other epitope is located at the position distant from the RBS. MAb that recognizes this epitope interfered with the hemagglutinin-fusion (H-F) interaction. This epitope was suggested to play a key role for formation of a higher order of an H-F protein oligomeric structure. Our data also identified one nonconserved effective neutralizing epitope. This epitope has been masked by an N-linked sugar modification in some genotype MV strains.
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Report
(5 results)
Research Products
(39 results)
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[Journal Article] The host protease TMPRSS2 plays a major role for in vivo replication of emerging H7N9 and seasonal influenza viruses.2014
Author(s)
Sakai K, Ami Y, Tahara M, Kubota T, Anraku M, Abe M, Nakajima N, Sekizuka T, Shirato K, Suzaki Y, Ainai A, Nakatsu Y, Kanou K, Nakamura K, Suzuki T, Komase K, Nobusawa E, Maenaka K, Kuroda M, Hasegawa H, Kawaoka Y, Tashiro M, Takeda M.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] The receptor-binding site of the measles virus hemagglutinin protein itself constitutes a conserved neutralizing epitope2013
Author(s)
Tahara M, Ohno S, Sakai K, Ito Y, Fukuhara H, Komase K, Brindley MA, Rota PA, Plemper RK, Maenaka K, Takeda M.
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Journal Title
J Virol.
Volume: 87巻
Issue: 6
Pages: 3583-3586
DOI
Related Report
Peer Reviewed
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[Journal Article] Functional and structural characterization of neutralizing epitopes of measles virus hemagglutinin protein2013
Author(s)
Tahara M, Ito Y, Brindley MA, Ma X, He J, Xu S, Fukuhara H, Sakai K, Komase K, Rota PA, Plemper RK, Maenaka K, Takeda M.
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Journal Title
J Virol.
Volume: 87巻
Issue: 1
Pages: 666-675
DOI
Related Report
Peer Reviewed
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[Journal Article] TMPRSS2 is an activating protease for respiratory parainfluenza viruses.2013
Author(s)
Abe M, Tahara M, Sakai K, Yamaguchi H, Kanou K, Shirato K, Kawase M, Noda M, Kimura H, Matsuyama S, Fukuhara H, Mizuta K, Maenaka K, Ami Y, Esumi M, Kato A, Takeda M.
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Journal Title
J Virol
Volume: 87
Issue: 21
Pages: 11930-11935
DOI
Related Report
Peer Reviewed
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[Journal Article] Canine distemper virus associated with a lethal outbreak in monkeys can readily adapt to use human receptors.2013
Author(s)
Sakai K, Yoshikawa T, Seki F, Fukushi S, Tahara M, Nagata N, Ami Y, Mizutani T, Kurane I, Yamaguchi R, Hasegawa H, Saijo M, Komase K, Morikawa S, Takeda M.
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Journal Title
J Virol
Volume: 87
Issue: 12
Pages: 7170-7175
DOI
Related Report
Peer Reviewed
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[Presentation] Importance of the P2 and P3 residues of fusion proteins of respiratory viruses for their proteolytic activation by TMPRSS22013
Author(s)
Maino Tahara, Masako Abe, Kouji Sakai, Kazuya Shirato, Kazuhiko Kanou1, Shutoku Matsuyama1, Hideo Fukuhara, Katsumi Maenaka, Yasushi Ami, Mariko Esumi, Atsushi Kato, Makoto Takeda
Organizer
NSV Meeting 2013
Place of Presentation
Granada, Spain
Related Report
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[Presentation] High potential of canine distemper virus in the ability to use macaca and human receptors2013
Author(s)
Kouji Sakai, Fumio Seki, Maino Tahara, Noriyuki Otsuki, Yasushi Ami, Ryoji Yamaguchi, Masayuki Saijo, Katsuhiro Komase, Shigeru Morikawa, Makoto Takeda
Organizer
NSV Meeting 2013
Place of Presentation
Granada, Spain
Related Report
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[Presentation] A structural and biochemical basis for the single serotype nature of MV2012
Author(s)
Tahara M, Ito Y, Brindley MA, Ma XM, He JL, Xu ST, Fukuhara H, Sakai K, Ohno S, Komase K, Rota PA, Plemper RK, Maenaka K, and Takeda M
Organizer
The 11th Awaji International Forum on Infection and Immunity
Place of Presentation
Hyogo, Japan
Related Report
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[Presentation] Virological, Structural and Molecular Bases for the Unchanged Antigenicity of Measles Virus2012
Author(s)
Tahara M, Ito Y, Brindley MA, Ma XM, He JL, Xu ST, Fukuhara H, Sakai K, Ohno S, Komase K, Rota PA, Plemper RK, Maenaka K, and Takeda M.
Organizer
Osong Symposium on Infectious Disease
Place of Presentation
韓国
Related Report
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[Presentation] 麻疹ウイルス単一血清型決定の分子基盤2012
Author(s)
田原舞乃, Melinda A. Brindley, 福原秀雄, 酒井宏治, 大野真治, 駒瀬勝啓, Paul A. Rota , Richard K. Plemper, 前仲勝実, 竹田誠
Organizer
第60回日本ウイルス学会学術集会
Place of Presentation
大阪
Related Report
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