Royal Jelly Stimulates Bone Formation: Physiologic and Nutrigenomic Studies with Mice and Cell Lines

  • NARITA Yukio
    Nagaragawa Research Center, API Co., Ltd.
  • NOMURA Johji
    Faculty of Pharmaceutical Science, Kumamoto University
  • OHTA Shozo
    Nagaragawa Research Center, API Co., Ltd.
  • INOH Yoshikazu
    Nagaragawa Research Center, API Co., Ltd.
  • SUZUKI Kazu-Michi
    Nagaragawa Research Center, API Co., Ltd.
  • ARAKI Yoko
    Nagaragawa Research Center, API Co., Ltd.
  • OKADA Shinji
    Graduate School of Agricultural and Life Sciences, Department of Applied Biological Chemistry, The University of Tokyo
  • MATSUMOTO Ichiro
    Graduate School of Agricultural and Life Sciences, Department of Applied Biological Chemistry, The University of Tokyo
  • ISOHAMA Yoichiro
    Faculty of Pharmaceutical Science, Kumamoto University
  • ABE Keiko
    Graduate School of Agricultural and Life Sciences, Department of Applied Biological Chemistry, The University of Tokyo
  • MIYATA Takeshi
    Faculty of Pharmaceutical Science, Kumamoto University
  • MISHIMA Satoshi
    Nagaragawa Research Center, API Co., Ltd.

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抄録

Royal jelly (RJ) has diverse physiological and pharmacological functions. We observed its weak estrogenic activity in the previous study. RJ stimulated the proliferation of mouse osteoblast-like MC3T3-E1 cells at 0.1 mg/ml, and the effect was blocked by the specific estrogen receptor antagonist ICI 182,780. The addition of 0.1–1.0 mg/ml RJ enhanced collagen production in culture medium. Oral administration of RJ to normal female mice for 9 weeks increased the ash content of their tibiae. DNA microarray analysis revealed significant changes in gene expression related to extracellular matrix formation when the femurs of mice fed RJ were analyzed. Quantitative reverse transcriptase-PCR (RT-PCR) confirmed up-regulation of procollagen I α1 gene expression. These data suggest that RJ as a whole or some of its individual components stimulates production of type I collagen and other activities for bone formation through action on osteoblasts.

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