In a unilaterally denervated diaphragm, atrophy of fast-twitch glycolytic (FG) fibers, but not of fast-twitch oxidative glycolytic (FOG) or slow-twitch oxidative (SO) fibers, occurs. The inhibition of atrophy in FOG and SO fibers may be due to the effects of stretch stimuli caused by respiratory contraction of the contralateral diaphragm. We examined the effects of exercise (treadmill running) on the histochemical and biochemical properties of muscle fibers of the costal diaphragm in three groups. Eighteen rats were distributed in (i) a denervation + treadmill running (Dn ex) group, (ii) a denervation (Dn) group, and (iii) a control (Ct) group. After 4 weeks of running, we measured the expression of myosin heavy chain (MHC) isoforms and cross-sectional area of the diaphragm muscle. The expression of MHC 1 and MHC 2a increased 4 weeks after denervation. In contrast, the expression of MHC 2d decreased significantly. After 4 weeks of running, the cross-sectional area of FG fibers increased significantly (85.5%; P < 0.05) compared with that in the Dn group. We suggest that exercise enhances the morphological properties and myosin heavy chain isoforms of the denervated diaphragm. This exercise-mediated adaptation may be associated with the improvement of FG fibers in the denervated diaphragm.
DOI | 10.11648/j.ajbls.20140206.11 |
Published in | American Journal of Biomedical and Life Sciences (Volume 2, Issue 6, December 2014) |
Page(s) | 141-145 |
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2024. Published by Science Publishing Group |
Denervated Diaphragm Muscle, Treadmill Running, Stretching, Histochemistry, Myosin Heavy Chains Isoform
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APA Style
Hidetaka Imagita, Susumu Sakata, Akira Minematsu, Naohiko Kanemura, Hideki Moriyama, et al. (). Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm. American Journal of Biomedical and Life Sciences, 2(6), 141-145. https://doi.org/10.11648/j.ajbls.20140206.11
ACS Style
Hidetaka Imagita; Susumu Sakata; Akira Minematsu; Naohiko Kanemura; Hideki Moriyama, et al. Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm. Am. J. Biomed. Life Sci. , 2(6), 141-145. doi: 10.11648/j.ajbls.20140206.11
AMA Style
Hidetaka Imagita, Susumu Sakata, Akira Minematsu, Naohiko Kanemura, Hideki Moriyama, et al. Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm. Am J Biomed Life Sci. ;2(6):141-145. doi: 10.11648/j.ajbls.20140206.11
@article{10.11648/j.ajbls.20140206.11, author = {Hidetaka Imagita and Susumu Sakata and Akira Minematsu and Naohiko Kanemura and Hideki Moriyama and Hidenori Takemoto and Tadayuki Mita}, title = {Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm}, journal = {American Journal of Biomedical and Life Sciences}, volume = {2}, number = {6}, pages = {141-145}, doi = {10.11648/j.ajbls.20140206.11}, url = {https://doi.org/10.11648/j.ajbls.20140206.11}, eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbls.20140206.11}, abstract = {In a unilaterally denervated diaphragm, atrophy of fast-twitch glycolytic (FG) fibers, but not of fast-twitch oxidative glycolytic (FOG) or slow-twitch oxidative (SO) fibers, occurs. The inhibition of atrophy in FOG and SO fibers may be due to the effects of stretch stimuli caused by respiratory contraction of the contralateral diaphragm. We examined the effects of exercise (treadmill running) on the histochemical and biochemical properties of muscle fibers of the costal diaphragm in three groups. Eighteen rats were distributed in (i) a denervation + treadmill running (Dn ex) group, (ii) a denervation (Dn) group, and (iii) a control (Ct) group. After 4 weeks of running, we measured the expression of myosin heavy chain (MHC) isoforms and cross-sectional area of the diaphragm muscle. The expression of MHC 1 and MHC 2a increased 4 weeks after denervation. In contrast, the expression of MHC 2d decreased significantly. After 4 weeks of running, the cross-sectional area of FG fibers increased significantly (85.5%; P < 0.05) compared with that in the Dn group. We suggest that exercise enhances the morphological properties and myosin heavy chain isoforms of the denervated diaphragm. This exercise-mediated adaptation may be associated with the improvement of FG fibers in the denervated diaphragm.}, year = {} }
TY - JOUR T1 - Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm AU - Hidetaka Imagita AU - Susumu Sakata AU - Akira Minematsu AU - Naohiko Kanemura AU - Hideki Moriyama AU - Hidenori Takemoto AU - Tadayuki Mita Y1 - PY - N1 - https://doi.org/10.11648/j.ajbls.20140206.11 DO - 10.11648/j.ajbls.20140206.11 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 141 EP - 145 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20140206.11 AB - In a unilaterally denervated diaphragm, atrophy of fast-twitch glycolytic (FG) fibers, but not of fast-twitch oxidative glycolytic (FOG) or slow-twitch oxidative (SO) fibers, occurs. The inhibition of atrophy in FOG and SO fibers may be due to the effects of stretch stimuli caused by respiratory contraction of the contralateral diaphragm. We examined the effects of exercise (treadmill running) on the histochemical and biochemical properties of muscle fibers of the costal diaphragm in three groups. Eighteen rats were distributed in (i) a denervation + treadmill running (Dn ex) group, (ii) a denervation (Dn) group, and (iii) a control (Ct) group. After 4 weeks of running, we measured the expression of myosin heavy chain (MHC) isoforms and cross-sectional area of the diaphragm muscle. The expression of MHC 1 and MHC 2a increased 4 weeks after denervation. In contrast, the expression of MHC 2d decreased significantly. After 4 weeks of running, the cross-sectional area of FG fibers increased significantly (85.5%; P < 0.05) compared with that in the Dn group. We suggest that exercise enhances the morphological properties and myosin heavy chain isoforms of the denervated diaphragm. This exercise-mediated adaptation may be associated with the improvement of FG fibers in the denervated diaphragm. VL - 2 IS - 6 ER -