American Journal of Biomedical and Life Sciences

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Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm

Received: 03 November 2014    Accepted: 18 November 2014    Published:
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Abstract

In a unilaterally denervated diaphragm, atrophy of fast-twitch glycolytic (FG) fibers, but not of fast-twitch oxidative glycolytic (FOG) or slow-twitch oxidative (SO) fibers, occurs. The inhibition of atrophy in FOG and SO fibers may be due to the effects of stretch stimuli caused by respiratory contraction of the contralateral diaphragm. We examined the effects of exercise (treadmill running) on the histochemical and biochemical properties of muscle fibers of the costal diaphragm in three groups. Eighteen rats were distributed in (i) a denervation + treadmill running (Dn ex) group, (ii) a denervation (Dn) group, and (iii) a control (Ct) group. After 4 weeks of running, we measured the expression of myosin heavy chain (MHC) isoforms and cross-sectional area of the diaphragm muscle. The expression of MHC 1 and MHC 2a increased 4 weeks after denervation. In contrast, the expression of MHC 2d decreased significantly. After 4 weeks of running, the cross-sectional area of FG fibers increased significantly (85.5%; P < 0.05) compared with that in the Dn group. We suggest that exercise enhances the morphological properties and myosin heavy chain isoforms of the denervated diaphragm. This exercise-mediated adaptation may be associated with the improvement of FG fibers in the denervated diaphragm.

DOI 10.11648/j.ajbls.20140206.11
Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 6, December 2014)
Page(s) 141-145
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Denervated Diaphragm Muscle, Treadmill Running, Stretching, Histochemistry, Myosin Heavy Chains Isoform

References
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[2] M.I. Lewis, T.J. Lorusso, W.Z. Zhan and G.C. Sieck, “Interactive effects of denervation and malnutrition on diaphragm structure and function,”Journal of Applied Physiology, vol.81, no.5, pp.2165-2172, 1996.
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[6] W.Z. Zhan, G.A. Farkas, M.A. Schroeder, L.E. Gosselin and G.C. Sieck, “Regional adaptations of rabbit diaphragm muscle fibers to unilateral denervation,”Journal of Applied Physiology, vol.79, no.3, pp.941-950, 1995.
[7] H. Imagita, Y. Saeki, E. Shono, O. Yoshimura, Y. Tobimatsu and H. Miyata, “The effect of aging on functional and morphological changes in the denervated diaphragm muscle of rats,”Journal of the Japanese Physical Therapy, vol.33, no.1, pp.22-29, 2006.
[8] T. Morinaga (editing), “Exercise and Physiological Functions,”NAP limited, pp.108-122, 1999. (In Japanese)
[9] A. d'Albis, R. Couteaux, F. Goubel, C. Janmot and J.C. Mira, “Response to denervation of rabbit soleus and gastrocnemius muscles. Time-course study of postnatal changes in myosin isoforms, fiber types, and contractile properties,”Biology of the Cell, vol.85, no.1, pp.9-20, 1995.
[10] K.A. Huey and S.C. Bodine, “Changes in myosin mRNA and protein expression in denervated rat soleus and tibialis anterior,”European Journal of Biochemistry, vol.256, no.1, pp.45-50, 1998.
[11] P.C. Geiger, J.P. Bailey, W.Z. Zhan, C.B. Mantilla and G.C. Sieck, “Denervation-induced changes in myosin heavy chain expression in the rat diaphragm muscle,”Journal of Applied Physiology, vol.95, no.2, pp.611-619, 2003.
[12] T. Saito and H. Soya, “Changes of hypothalamic ACTH secretagogue (CRH/AVP) during running stress in rats,”Journal of Exercise and Sports Physiology, vol.12, no.1, pp.33-39, 2005. (In Japanese)
[13] T. Saito and H. Soya, “Delineation of responsive AVP-containing neurons to running stress in the hypothalamus,”American Journal of Physiology Regulatory, Integrative and Comparative Physiology, vol.286, no.3, pp.R484-R490, 2004.
[14] M. Okita, T. Yoshimura, J. Nakano, A. Saeki, A.Uehara, A. Mineshita and K. Eguchi, “Effects of Short Duration Stretching on Disuse Muscle Atrophy in Immobilized Rat Soleus Muscles,”Journal of the Japanese Physical Therapy Association, vol.4, no.1, pp. 1-5, 2001.
[15] T. Yamazaki, “Influence of Hindlimb Unweighting and Intermittent Weight Bearing on Dynamics of Nuclei in Rat Soleus Muscle,”Journal of the Japanese Physical Therapy Association, vol.6, no.1, pp. 1-8, 2003.
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Author Information
  • Graduate School of Health Sciences, Kio University, Nara, Japan

  • Graduate School of Health Sciences, Kio University, Nara, Japan

  • Graduate School of Health Sciences, Kio University, Nara, Japan

  • Department of Physical Therapy, Faculty of Health Sciences, Saitama Prefectural University, Saitama, Japan

  • Department of Physical Therapy, Faculty of Health Sciences, Kobe University, Hyogo, Japan

  • Department of Physical Therapy, Faculty of Health Sciences, Hiroshima Prefectural University, Hiroshima, Japan

  • Graduate School of Health Sciences, Kio University, Nara, Japan; Department of Rehabilitation, Akiyama Orthopedic Hospital, Mie, Japan

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    Hidetaka Imagita, Susumu Sakata, Akira Minematsu, Naohiko Kanemura, Hideki Moriyama, et al. (). Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm. American Journal of Biomedical and Life Sciences, 2(6), 141-145. https://doi.org/10.11648/j.ajbls.20140206.11

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    ACS Style

    Hidetaka Imagita; Susumu Sakata; Akira Minematsu; Naohiko Kanemura; Hideki Moriyama, et al. Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm. Am. J. Biomed. Life Sci. , 2(6), 141-145. doi: 10.11648/j.ajbls.20140206.11

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    AMA Style

    Hidetaka Imagita, Susumu Sakata, Akira Minematsu, Naohiko Kanemura, Hideki Moriyama, et al. Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm. Am J Biomed Life Sci. ;2(6):141-145. doi: 10.11648/j.ajbls.20140206.11

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  • @article{10.11648/j.ajbls.20140206.11,
      author = {Hidetaka Imagita and Susumu Sakata and Akira Minematsu and Naohiko Kanemura and Hideki Moriyama and Hidenori Takemoto and Tadayuki Mita},
      title = {Effects of Exercise on Fiber Properties in the Denervated Rodent Diaphragm},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {2},
      number = {6},
      pages = {141-145},
      doi = {10.11648/j.ajbls.20140206.11},
      url = {https://doi.org/10.11648/j.ajbls.20140206.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbls.20140206.11},
      abstract = {In a unilaterally denervated diaphragm, atrophy of fast-twitch glycolytic (FG) fibers, but not of fast-twitch oxidative glycolytic (FOG) or slow-twitch oxidative (SO) fibers, occurs. The inhibition of atrophy in FOG and SO fibers may be due to the effects of stretch stimuli caused by respiratory contraction of the contralateral diaphragm. We examined the effects of exercise (treadmill running) on the histochemical and biochemical properties of muscle fibers of the costal diaphragm in three groups. Eighteen rats were distributed in (i) a denervation + treadmill running (Dn ex) group, (ii) a denervation (Dn) group, and (iii) a control (Ct) group. After 4 weeks of running, we measured the expression of myosin heavy chain (MHC) isoforms and cross-sectional area of the diaphragm muscle. The expression of MHC 1 and MHC 2a increased 4 weeks after denervation. In contrast, the expression of MHC 2d decreased significantly. After 4 weeks of running, the cross-sectional area of FG fibers increased significantly (85.5%; P < 0.05) compared with that in the Dn group. We suggest that exercise enhances the morphological properties and myosin heavy chain isoforms of the denervated diaphragm. This exercise-mediated adaptation may be associated with the improvement of FG fibers in the denervated diaphragm.},
     year = {}
    }
    

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    AU  - Hidetaka Imagita
    AU  - Susumu Sakata
    AU  - Akira Minematsu
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    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
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    PB  - Science Publishing Group
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    AB  - In a unilaterally denervated diaphragm, atrophy of fast-twitch glycolytic (FG) fibers, but not of fast-twitch oxidative glycolytic (FOG) or slow-twitch oxidative (SO) fibers, occurs. The inhibition of atrophy in FOG and SO fibers may be due to the effects of stretch stimuli caused by respiratory contraction of the contralateral diaphragm. We examined the effects of exercise (treadmill running) on the histochemical and biochemical properties of muscle fibers of the costal diaphragm in three groups. Eighteen rats were distributed in (i) a denervation + treadmill running (Dn ex) group, (ii) a denervation (Dn) group, and (iii) a control (Ct) group. After 4 weeks of running, we measured the expression of myosin heavy chain (MHC) isoforms and cross-sectional area of the diaphragm muscle. The expression of MHC 1 and MHC 2a increased 4 weeks after denervation. In contrast, the expression of MHC 2d decreased significantly. After 4 weeks of running, the cross-sectional area of FG fibers increased significantly (85.5%; P < 0.05) compared with that in the Dn group. We suggest that exercise enhances the morphological properties and myosin heavy chain isoforms of the denervated diaphragm. This exercise-mediated adaptation may be associated with the improvement of FG fibers in the denervated diaphragm.
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