書誌事項
- タイトル別名
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- Development of New Methods for Synthesis of Artificial Nucleic Acids having Various Functional Groups
- キノウセイ ジンコウ カクサン ノ ソウセイ オ メザシタ シンキ カクサン ゴウセイホウ ノ カイハツ
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抄録
Development of effective methods for the synthesis of modified oligonucleotides having various functional groups is highly desirable today. Recently, we developed O-selective phosphorylation named the proton-block method and the activated phosphite method to synthesize modified DNA oligonucleotide having alkaline-labile functional groups. In RNA synthesis without base protection, it was found that O-selectivity of the phosphorylation with P-N bond cleavage using 6-nitro-HOBt was more than 99%. Additionally, development of new protecting groups, which can be removed under mild conditions, such as AzMB, PSC and amidine-type groups was carried out. Furthermore, we developed the effective pyrophosphorylation using O-N phosphoryl rearrangement of phosphoramidite compound mediated by HOBt derivative. This new pyrophosphorylation could be applied to the synthesis of adenylpyrophosphoryl capped and 5’-terminal m32,2,7Gppp capped oligonucleotides.
収録刊行物
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- 有機合成化学協会誌
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有機合成化学協会誌 72 (8), 899-909, 2014
公益社団法人 有機合成化学協会
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詳細情報
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- CRID
- 1390282680316150016
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- NII論文ID
- 130004691490
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- NII書誌ID
- AN0024521X
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- ISSN
- 18836526
- 00379980
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- NDL書誌ID
- 025750134
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可