The Preparation of a Lipidic Endotoxin Affects Its Biological Activities
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- Okemoto Kazuo
- Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases
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- Hanada Kentaro
- Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases
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- Nishijima Masahiro
- Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases The National Institute of Health Sciences
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- Kawasaki Kiyoshi
- Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases Faculty of Pharmaceutical Sciences, Doshisha Women's College
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抄録
Bacterial membrane constituents, such as Ornithine-containing lipid (OL) and the lipid A portion of lipopolysaccharide, trigger various immune responses through recognition by Toll-like receptor (TLR) 4. Usually, these lipids are dissolved in a small amount of aqueous or organic solvent before being added to the culture medium for examination of their biological activities. Macrophages stimulated with OL or lipid A sonically dissolved in saline released both interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In contrast, macrophages stimulated with OL or lipid A sonically dissolved in ethanol or dimethyl sulfoxide (DMSO) secreted much TNF-α, but very little IL-1β. These results, taken together, indicate that how an endotoxin is prepared affects its biological activities. In addition, electromicroscopic analysis revealed that sonication of air-dried OL or lipid A in DMSO produced larger particles than those produced in saline, suggesting that the process of preparing lipidic TLR4-ligands affects their physical state including particle size, and that the physical state might be an important determinant of biological activity.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 31 (10), 1952-1954, 2008
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詳細情報
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- CRID
- 1390282679601047168
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- NII論文ID
- 110006936340
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 9655688
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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