Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Notes
RNA Aptamers for Targeting Mitochondria Using a Mitochondria-Based SELEX Method
Yuri TawarayaMamoru HyodoMst Naznin AraYuma YamadaHideyoshi Harashima
Author information
JOURNAL FREE ACCESS FULL-TEXT HTML

2014 Volume 37 Issue 8 Pages 1411-1415

Details
Abstract

The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2′-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.

Content from these authors
© 2014 The Pharmaceutical Society of Japan
Previous article Next article
feedback
Top