Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3
抄録
The intranuclear disposition of plasmid DNA is extremely important for transgene expression. Exogenous histones have been used as carriers of plasmid DNA in histone-mediated gene delivery. In this study, the effects of exogenous histone H3 complexed with plasmid DNA on transgene expression efficiency were examined. The plasmid-histone complexes in various ratios were transfected into HeLa cells by osmotic pressure. Histone H3 suppressed transgene expression in the nucleus in a dose-dependent manner. Our results suggest that the histone-mediated gene delivery is unlikely to be useful, from the viewpoint of the intranuclear disposition.
収録刊行物
-
- International Journal of Pharmaceutics
-
International Journal of Pharmaceutics 392 (1-2), 249-253, 2010-06-15
Elsevier B.V.
- Tweet
詳細情報
-
- CRID
- 1050001339005869056
-
- NII論文ID
- 120002208933
-
- HANDLE
- 2115/43144
-
- ISSN
- 03785173
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- IRDB
- CiNii Articles