RNA Aptamers for Targeting Mitochondria Using a Mitochondria-Based SELEX Method
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- Tawaraya Yuri
- Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University
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- Hyodo Mamoru
- Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University
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- Ara Mst Naznin
- Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University
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- Yamada Yuma
- Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University
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- Harashima Hideyoshi
- Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University
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The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2′-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 37 (8), 1411-1415, 2014
公益社団法人 日本薬学会
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詳細情報
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- CRID
- 1390282679608918016
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- NII論文ID
- 130004677537
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC2cbos1KhsQ%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 025610303
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- PubMed
- 25087963
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可