Global Gene Expression in Bradyrhizobium japonicum Cultured with Vanillin, Vanillate, 4-Hydroxybenzoate and Protocatechuate
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- Ito Naofumi
- Graduate School of Life Sciences, Tohoku University
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- Itakura Manabu
- Graduate School of Life Sciences, Tohoku University
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- Eda Shima
- Graduate School of Life Sciences, Tohoku University
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- Saeki Kazuhiko
- Department of Biological Science, Faculty of Science, Nara Women's University
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- Oomori Hirofumi
- Department of Biology Graduate School of Science, Osaka University
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- Yokoyama Tadashi
- Tokyo University of Agriculture and Technology
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- Kaneko Takakazu
- Kazusa DNA Research Institute
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- Tabata Satoshi
- Kazusa DNA Research Institute
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- Ohwada Takuji
- Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine
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- Tajima Shigeyuki
- Department of Life Science, Kagawa University
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- Uchiumi Toshiki
- Department of Chemistry and BioScience, Faculty of Science, Kagoshima University
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- Masai Eiji
- Department of Bioengineering, Nagaoka University of Technology
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- Tsuda Masataka
- Graduate School of Life Sciences, Tohoku University
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- Mitsui Hisayuki
- Graduate School of Life Sciences, Tohoku University
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- Minamisawa Kiwamu
- Graduate School of Life Sciences, Tohoku University
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Pathways for aerobic degradation of naturally occurring aromatics were estimated from the entire genome sequence of Bradyrhizobium japonicum strain USDA110, a symbiotic nitrogen-fixing bacterium in soil. Many homologs for the genes encoding various oxygenases and enzymes for the β-ketoadipate pathway in the degradation of vanillin, vanillate, protocatechuate, and 4-hydroxybenzoate were scattered over nine loci of the genome. Using a macroarray developed for B. japonicum strain USDA110, we compared gene expression profiles in cells grown in each of these aromatic compounds as a sole carbon source with those of succinate-fed cells. One set of oxygenase genes homologous to pcaGH, pobA, and vanAB and structurally accompanied by transcriptional regulator homologs was markedly upregulated in their expression by one or more of the four aromatics, whereas no marked change was observed in the expression levels of pcaBCDIJF genes for the β-ketoadipate pathway. In addition, cells fed vanillin and vanillate showed high levels of expression of genes for a glutathione-dependent pathway of formaldehyde oxidation, suggesting that the formaldehyde generated from vanillate's demethylation is oxidized via C1 metabolism in B. japonicum. The expression of the above genes was confirmed by quantitative reverse transcription PCR. The implications of these results are discussed in terms of degradation pathways, gene regulation, and the soil environment.<br>
収録刊行物
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- Microbes and environments
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Microbes and environments 21 (4), 240-250, 2006
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報
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- CRID
- 1390001204344840320
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- NII論文ID
- 110006151689
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 8587172
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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