Relationship Between Soil Type and N₂O Reductase Genotype (nosZ) of Indigenous Soybean Bradyrhizobia : nosZ-minus Populations are Dominant in Andosols
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- Shiina Yoko
- Graduate School of Life Sciences, Tohoku University
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- Itakura Manabu
- Graduate School of Life Sciences, Tohoku University
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- Choi Hyunseok
- Graduate School of Life Sciences, Tohoku University
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- Saeki Yuichi
- Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University
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- Hayatsu Masahito
- National Institute for Agro-Environmental Sciences
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- Minamisawa Kiwamu
- Graduate School of Life Sciences, Tohoku University
書誌事項
- タイトル別名
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- Relationship Between Soil Type and N<sub>2</sub>O Reductase Genotype (<i>nosZ</i>) of Indigenous Soybean Bradyrhizobia: <i>nosZ</i>-minus Populations are Dominant in Andosols
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抄録
Bradyrhizobium japonicum strains that have the nosZ gene, which encodes N2O reductase, are able to mitigate N2O emissions from soils (15). To examine the distribution of nosZ genotypes among Japanese indigenous soybean bradyrhizobia, we isolated bradyrhizobia from the root nodules of soybean plants inoculated with 32 different soils and analyzed their nosZ and nodC genotypes. The 1556 resultant isolates were classified into the nosZ+/nodC+ genotype (855 isolates) and nosZ−/nodC+ genotype (701 isolates). The 11 soil samples in which nosZ− isolates significantly dominated (P < 0.05; the χ2 test) were all Andosols (a volcanic ash soil prevalent in agricultural fields in Japan), whereas the 17 soil samples in which nosZ+ isolates significantly dominated were mainly alluvial soils (non-volcanic ash soils). This result was supported by a principal component analysis of environmental factors: the dominance of the nosZ− genotype was positively correlated with total N, total C, and the phosphate absorption coefficient in the soils, which are soil properties typical of Andosols. Internal transcribed spacer sequencing of representative isolates showed that the nosZ+ and nosZ− isolates of B. japonicum fell mainly into the USDA110 (BJ1) and USDA6 (BJ2) groups, respectively. These results demonstrated that the group lacking nosZ was dominant in Andosols, which can be a target soil type for an N2O mitigation strategy in soybean fields. We herein discussed how the nosZ genotypes of soybean bradyrhizobia depended on soil types in terms of N2O respiration selection and genomic determinants for soil adaptation.
収録刊行物
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- Microbes and environments
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Microbes and environments 29 (4), 420-426, 2014
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報
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- CRID
- 1390001204346395392
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- NII論文ID
- 40020304074
- 130004703875
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 025987370
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- PubMed
- 25476067
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可