Metagenomic analysis of bacterial community associated with taproot of sugar beet
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- Tsurumaru Hirohito
- Graduate School of Life Science, Tohoku University
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- Okubo Takashi
- Graduate School of Life Science, Tohoku University
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- Okazaki Kazuyuki
- Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization
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- Hashimoto Megumi
- Graduate School of Life Science, Tohoku University
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- Kakizaki Kaori
- Graduate School of Life Science, Tohoku University
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- Hanzawa Eiko
- Graduate School of Life Science, Tohoku University
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- Takahashi Hiroyuki
- Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization
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- Asanome Noriyuki
- Yamagata Integrated Agricultural Research Center, Yamagata
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- Tanaka Fukuyo
- Agricultural Research Center, National Agriculture and Food Research Organization
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- Sekiyama Yasuyo
- National Food Research Institute, National Agriculture and Food Research Organization
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- Ikeda Seishi
- Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization
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- Minamisawa Kiwamu
- Graduate School of Life Science, Tohoku University
書誌事項
- タイトル別名
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- Metagenomic Analysis of the Bacterial Community Associated with the Taproot of Sugar Beet
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抄録
We analyzed a metagenome of the bacterial community associated with the taproot of sugar beet (Beta vulgaris L.) in order to investigate the genes involved in plant growth-promoting traits (PGPTs), namely 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, indole acetic acid (IAA), N2 fixation, phosphate solubilization, pyrroloquinoline quinone, siderophores, and plant disease suppression as well as methanol, sucrose, and betaine utilization. The most frequently detected gene among the PGPT categories encoded β-1,3-glucanase (18 per 105 reads), which plays a role in the suppression of plant diseases. Genes involved in phosphate solubilization (e.g., for quinoprotein glucose dehydrogenase), methanol utilization (e.g., for methanol dehydrogenase), siderophore production (e.g. isochorismate pyruvate lyase), and ACC deaminase were also abundant. These results suggested that such PGPTs are crucially involved in supporting the growth of sugar beet. In contrast, genes for IAA production (iaaM and ipdC) were less abundant (~1 per 105 reads). N2 fixation genes (nifHDK) were not detected; bacterial N2 -fixing activity was not observed in the 15N2 -feeding experiment. An analysis of nitrogen metabolism suggested that the sugar beet microbiome mainly utilized ammonium and nitroalkane as nitrogen sources. Thus, N2 fixation and IAA production did not appear to contribute to sugar beet growth. Taxonomic assignment of this metagenome revealed the high abundance of Mesorhizobium, Bradyrhizobium, and Streptomyces, suggesting that these genera have ecologically important roles in the taproot of sugar beet. Bradyrhizobium-assigned reads in particular were found in almost all categories of dominant PGPTs with high abundance. The present study revealed the characteristic functional genes in the taproot-associated microbiome of sugar beet, and suggest the opportunity to select sugar beet growth-promoting bacteria.
収録刊行物
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- Microbes and environments
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Microbes and environments 30 (1), 63-69, 2015
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報
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- CRID
- 1390282679322908160
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- NII論文ID
- 130005061718
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 026292516
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- PubMed
- 25740621
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可