Metagenomic Analysis Revealed Methylamine and Ureide Utilization of Soybean-Associated Methylobacterium
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- Minami Tomoyuki
- Graduate School of Life Sciences, Tohoku University
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- Anda Misue
- Graduate School of Life Sciences, Tohoku University
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- Mitsui Hisayuki
- Graduate School of Life Sciences, Tohoku University
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- Sugawara Masayuki
- Graduate School of Life Sciences, Tohoku University
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- Kaneko Takakazu
- Kazusa DNA Research Institute
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- Sato Shusei
- Graduate School of Life Sciences, Tohoku University Kazusa DNA Research Institute
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- Ikeda Seishi
- Graduate School of Life Sciences, Tohoku University
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- Okubo Takashi
- Graduate School of Life Sciences, Tohoku University
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- Tsurumaru Hirohito
- Graduate School of Life Sciences, Tohoku University
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- Minamisawa Kiwamu
- Graduate School of Life Sciences, Tohoku University
書誌事項
- タイトル別名
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- Metagenomic Analysis Revealed Methylamine and Ureide Utilization of Soybean-Associated <i>Methylobacterium</i>
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<p>Methylobacterium inhabits the phyllosphere of a large number of plants. We herein report the results of comparative metagenome analyses on methylobacterial communities of soybean plants grown in an experimental field in Tohoku University (Kashimadai, Miyagi, Japan). Methylobacterium was identified as the most dominant genus (33%) among bacteria inhabiting soybean stems. We classified plant-derived Methylobacterium species into Groups I, II, and III based on 16S rRNA gene sequences, and found that Group I members (phylogenetically close to M. extorquens) were dominant in soybean-associated Methylobacterium. By comparing 29 genomes, we found that all Group I members possessed a complete set of genes for the N-methylglutamate pathway for methylamine utilization, and genes for urea degradation (urea carboxylase, urea amidolyase, and conventional urease). Only Group I members and soybean methylobacterial isolates grew in a culture supplemented with methylamine as the sole carbon source. They utilized urea or allantoin (a urea-related compound in legumes) as the sole nitrogen source; however, group III also utilized these compounds. The utilization of allantoin may be crucial in soybean-bacterial interactions because allantoin is a transported form of fixed nitrogen in legume plants. Soybean-derived Group I strain AMS5 colonized the model legume Lotus japonicus well. A comparison among the 29 genomes of plant-derived and other strains suggested that several candidate genes are involved in plant colonization such as csgG (curli fimbriae). Genes for the N-methylglutamate pathway and curli fimbriae were more abundant in soybean microbiomes than in rice microbiomes in the field. Based on these results, we discuss the lifestyle of Methylobacterium in the legume phyllosphere.</p>
収録刊行物
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- Microbes and environments
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Microbes and environments 31 (3), 268-278, 2016
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報
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- CRID
- 1390001204345167360
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- NII論文ID
- 40020943102
- 130005265032
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 027619121
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- PubMed
- 27431374
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可