Abstract
ANGUSTIFOLIA (AN), a plant homolog of C-terminal binding protein, controls the polar elongation of leaf cells and the trichome-branching pattern in Arabidopsis thaliana. In the present study, degenerate PCR was used to isolate an ortholog of AN, referred to as LgAN, from larch (Larix gmelinii). The LgAN cDNA is predicted to encode a protein of 646 amino acids that shows striking sequence similarity to AN proteins from other plants. The predicted amino acid sequence has a conserved NAD-dependent 2-hydroxy acid dehydrogenase (D2-HDH) motif and a plant AN-specific LxCxE/D motif at its N-terminus, as well as a plant-specific long C-terminal region. The LgAN gene is a single-copy gene that is expressed in all larch tissues. Expression of the LgAN cDNA rescued the leaf width and trichome-branching pattern defects in the angustifolia-1 (an-1) mutant of Arabidopsis, showing that the LgAN gene has effects complementary to those of AN. These results suggest that the LgAN gene has the same function as the AN gene.
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Abbreviations
- AN :
-
ANGUSTIFOLIA
- CtBP:
-
Carboxy-terminal binding protein
- D2-HDH:
-
NAD-dependent 2-hydroxy acid dehydrogenase
- IAN :
-
Ipomoea nil ANGUSTIFOLIA
- LgAN :
-
Larix gmelinii ANGUSTIFOLIA
- MAN :
-
Marchantia polymorpha ANGUSTIFOLIA
- NLS:
-
Nuclear localization signal
- NTPII:
-
Neomycin phosphortransferase II gene
- PpAN :
-
Physcomitrella patens ANGUSTIFOLIA
- RACE:
-
Rapid amplification of cDNA ends
- RT-PCR:
-
Reverse transcription-polymerase chain reaction
References
Akama K, Shiraishi H, Ohta S, Nakamura K, Okada K, Shimura Y (1992) Efficient transformation of Arabidopsis thaliana: comparison of the efficiencies with various organs, plant ecotypes and Agrobacterium strains. Plant Cell Rep 12:7–11
Brannon M, Brown JD, Bates R, Kimelman D, Moon RT (1999) XCtBP is a XTcf-3 co-repressor with roles throughout Xenopus development. Development 126:3159–3170
Chang S, Puryear T, Caimey I (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Bio Rep 11:113–116
Cho K, Shindo T, Kim G, Nitasaka E, Tsukaya H (2005) Characterization of a member of the AN subfamily, IAN, from Ipomoea nil. Plant Cell Physiol 46:250–255
Folkers U, Kirik V, Schobinger U, Falk S, Krishnakumar S, Pollock MA, Oppenheimer DG, Day I, Reddy AS, Jurgens G, Hülskamp M, Reddy AR (2002) The cell morphogenesis gene ANGUSTIFOLIA encodes a CtBP/BARS-like protein and is involved in the control of the microtubule cytoskeleton. EMBO J 21:1280–1288
Furusawa T, Moribe H, Kondoh H, Higashi Y (1999) Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomaim factor deltaEF1. Mol Cell Biol 19:8581–8590
Hulskamp M, Misera E (1994) Genetic dissection of trichome cell development in Arabidopsis. Cell 76:555–566
Kim G, Tsukaya H, Saito Y, Uchimiya H (1999) Changes in the shapes of leaves and flowers upon overexpression of the novel cytochrome P450 in Arabidopsis thaliana. Proc Natl Acad Sci USA 96:9433–9437
Kim G, Shoda K, Tsuge T, Cho K, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H (2002) The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation. EMBO J 21:1267–1279
Lin XF, Zhang WB, Takano H, Takio S, Ono K (2004) Efficient plant regeneration and micropropagation from callus derived from mature zygotic embryo of Larix gmelinii. Plant Biotechnol 21:159–163
Lin XF, Zhang WB, Takechi K, Takio S, Ono K, Takano H (2005) Stable genetic transformation of Larix gmelinii L. by particle bombardment of zygotic embryos. Plant Cell Rep 24:418–425
Nibu Y, Zhang H, Bajor E, Barolo S, Small S, Levine M (1998a) dCtBP mediates transcriptional repression by Knirps, Kruppel and Snail in the Drosophila embryo. EMBO J 17:7009–7020
Nibu Y, Zhang H, Levine M (1998b) Interaction of short-range repressors with Drosophila dCtBP in the embryo. Science 280:101–104
Page RD (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Schaper U, Boyd JM, Verma S, Uhlmann E, Subbramanian T, Channadurai G (1995) Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation. Proc Natl Acad Sci USA 92:10467–10471
Stern MD, Aihara H, Cho KH, Kim GT, Horiguchi G, Roccaro GA, Guevara E, Sun HH, Neggri D, Tsukaya H, Nibu Y (2007) Structurally related Arabidopsis ANGUSTIFORLIA is functionally distinct from the transcriptional corepressor CtBP. Dev Genes Evol 217:759–769
Thompson J, Higgins D, Gibson T (1994) CLUSTAL-W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting position specific gap penalties and weight matrix choice. Nucleic Acid Res 22:4673–4680
Tsuge T, Tsukaya H, Uchimiya H (1996) Two independent and polarized processes of cell elongation regulate leaf blade expansion in Arabidopsis thaliana (L.) Heynh. Development 122:1589–1600
Tsukaya H (2006) Mechanism of leaf shape determination. Ann Rev Plant Biol 57:477–496
Tsukaya H, Tsuge T, Uchimiya H (1994) The cotyledon: a superior system for studies of leaf development. Planta 195:309–312
Acknowledgments
The authors thank Dr. Gorou Horiguchi of the University of Tokyo for helpful technical guidance. This study was supported by Grants-in-Aid for Scientific Research (a Creative Scientific Research grant and Grant-in-Aid for Scientific Research on Priority Areas to H. Tsukaya and H. Takano, and number 19570060 to H. Takano and H. Tsukaya) from the Ministry of Education, Science, Sports and Culture of Japan and from the Toray Science Foundation to H. Tsukaya.
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The nucleotide sequence reported in this paper has been submitted to the DNA databank of Japan (DDBJ) under accession number AB373990.
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Lin, X., Minamisawa, N., Takechi, K. et al. Isolation and characterization of the Larix gmelinii ANGUSTIFOLIA (LgAN) gene. Planta 228, 601–608 (2008). https://doi.org/10.1007/s00425-008-0762-9
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DOI: https://doi.org/10.1007/s00425-008-0762-9