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Isolation and characterization of the Larix gmelinii ANGUSTIFOLIA (LgAN) gene

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Abstract

ANGUSTIFOLIA (AN), a plant homolog of C-terminal binding protein, controls the polar elongation of leaf cells and the trichome-branching pattern in Arabidopsis thaliana. In the present study, degenerate PCR was used to isolate an ortholog of AN, referred to as LgAN, from larch (Larix gmelinii). The LgAN cDNA is predicted to encode a protein of 646 amino acids that shows striking sequence similarity to AN proteins from other plants. The predicted amino acid sequence has a conserved NAD-dependent 2-hydroxy acid dehydrogenase (D2-HDH) motif and a plant AN-specific LxCxE/D motif at its N-terminus, as well as a plant-specific long C-terminal region. The LgAN gene is a single-copy gene that is expressed in all larch tissues. Expression of the LgAN cDNA rescued the leaf width and trichome-branching pattern defects in the angustifolia-1 (an-1) mutant of Arabidopsis, showing that the LgAN gene has effects complementary to those of AN. These results suggest that the LgAN gene has the same function as the AN gene.

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Abbreviations

AN :

ANGUSTIFOLIA

CtBP:

Carboxy-terminal binding protein

D2-HDH:

NAD-dependent 2-hydroxy acid dehydrogenase

IAN :

Ipomoea nil ANGUSTIFOLIA

LgAN :

Larix gmelinii ANGUSTIFOLIA

MAN :

Marchantia polymorpha ANGUSTIFOLIA

NLS:

Nuclear localization signal

NTPII:

Neomycin phosphortransferase II gene

PpAN :

Physcomitrella patens ANGUSTIFOLIA

RACE:

Rapid amplification of cDNA ends

RT-PCR:

Reverse transcription-polymerase chain reaction

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Acknowledgments

The authors thank Dr. Gorou Horiguchi of the University of Tokyo for helpful technical guidance. This study was supported by Grants-in-Aid for Scientific Research (a Creative Scientific Research grant and Grant-in-Aid for Scientific Research on Priority Areas to H. Tsukaya and H. Takano, and number 19570060 to H. Takano and H. Tsukaya) from the Ministry of Education, Science, Sports and Culture of Japan and from the Toray Science Foundation to H. Tsukaya.

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Correspondence to Xiaofei Lin.

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The nucleotide sequence reported in this paper has been submitted to the DNA databank of Japan (DDBJ) under accession number AB373990.

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Lin, X., Minamisawa, N., Takechi, K. et al. Isolation and characterization of the Larix gmelinii ANGUSTIFOLIA (LgAN) gene. Planta 228, 601–608 (2008). https://doi.org/10.1007/s00425-008-0762-9

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  • DOI: https://doi.org/10.1007/s00425-008-0762-9

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