A loss-of-function mutation in the DWARF4/PETANKO5 gene enhances the late-flowering and semi-dwarf phenotypes of the Arabidopsis clock mutant lhy-12;cca1-101 under continuous light without affecting FLC expression
-
- Suzuki Syunji
- Gene Research Center, University of Tsukuba
-
- Miyata Kana
- Gene Research Center, University of Tsukuba Department of Natural Sciences, International Christian University
-
- Hara Miyuki
- Gene Research Center, University of Tsukuba Department of Natural Sciences, International Christian University
-
- Niinuma Kanae
- Gene Research Center, University of Tsukuba
-
- Tsukaya Hirokazu
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo Bio-Next Project, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences
-
- Takase Masahide
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo
-
- Hayama Ryosuke
- Department of Natural Sciences, International Christian University
-
- Mizoguchi Tsuyoshi
- Gene Research Center, University of Tsukuba Department of Natural Sciences, International Christian University
書誌事項
- タイトル別名
-
- A loss-of-function mutation in the <i>DWARF4</i>/<i>PETANKO5</i> gene enhances the late-flowering and semi-dwarf phenotypes of the <i>Arabidopsis</i> clock mutant <i>lhy-12;cca1-101</i> under continuous light without affecting <i>FLC</i> expression
この論文をさがす
抄録
The circadian clock plays important roles in the control of photoperiodic flowering in Arabidopsis. Mutations in the LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) genes (lhy;cca1) accelerate flowering under short days, whereas lhy;cca1 delays flowering under continuous light (LL). The lhy;cca1 mutant also exhibits short hypocotyls and petioles under LL. However, the molecular mechanisms underlying the regulation of both flowering time and organ lengths in the LHY/CCA1-dependent pathway are not fully understood. To address these questions, we performed EMS mutagenesis of the lhy-12;cca1-101 line and screened for mutations that enhance the lhy;cca1 phenotypes under LL. In this screen, we identified a novel allele of dwarf4 (dwf4) and named it petanko 5 (pta5). A similar level of enhancement of the delay in flowering was observed in these two dwf4 mutants when combined with the lhy;cca1 mutations. The lhy;cca1 and dwf4 mutations did not significantly affect the expression level of the floral repressor gene FLC under LL. Our results suggest that a defect in brassinosteroid (BR) signaling delayed flowering independent of the FLC expression level, at least in plants with the lhy;cca1 mutation grown under LL. The dwf4/pta5 mutation did not enhance the late-flowering phenotype of plants overexpressing SVP under LL, suggesting that SVP and BR function in a common pathway that controls flowering time. Our results suggest that the lhy;cca1 mutant exhibits delayed flowering due to both the BR signaling-dependent and -independent pathways under LL.
収録刊行物
-
- Plant Biotechnology
-
Plant Biotechnology 33 (4), 315-321, 2016
日本植物バイオテクノロジー学会
- Tweet
キーワード
詳細情報
-
- CRID
- 1390001204329504000
-
- NII論文ID
- 130005281084
-
- NII書誌ID
- AA11250821
-
- ISSN
- 13476114
- 13424580
-
- NDL書誌ID
- 027729161
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可